Prevalence, antibiotic and oil resistance pattern of some bacterial isolates from burns

Abstract

Infection is an important cause of mortality in patients with burns. Rapid emergence of hospital pathogens and antibiotic-resistant organisms necessitate periodic evaluation of bacterial colonization patterns and antibiogram sensitivity in burn wards. Sixty isolates from wounds of burns were collected from two hospitals in Cairo, Egypt along the period of 12 months in 2013. Antibiotic sensitivity of these isolates was assessed by single disk diffusion method. Multi drug resistance percentage and the most prevalent resistance phenotype among bacterial isolates were recorded. In addition, 19 essential oils were tested against the MDR isolates. The most potent oils were analyzed by GC-MS to determine their main chemical constituents. According to microbiological and biochemical identification method, Pseudomonas aeruginosa was the most dominant organism 23 (38%), followed by Staphylococcus aureus 16 (27%), Klebsiella spp. 11 (18%), Acinetobacter spp. 4 (7%). Three isolates of Escherichia coli (5%) and three isolates of Proteus spp. (5%). Piperacillin-tazobactam, imipenem and linezolid antibiotics were the most effective antibiotics against Pseudomonas aeruginosa, Enterobacteriaceae and S. aureus isolates respectively. Cinnamon and thyme essential oils were the most potent oils against the multi drug resistant burn wound isolate. Cinnamaldehyde (60.7%) and ρ-cymene (50%) were the major chemical constituents in cinnamon and thyme essential oils, respectively. It is clear that antibiotic resistance levels are high among the examined bacterial isolates of burn wounds. This study could be useful for physician to better choice of empiric therapy. Cinnamon and thyme may be used as a promising an alternative medicine for the treatment of burn wound infections.

In Vitro Activities of Polymyxins Group Combined With Imipenem or Meropenem Against Carbapenem-Resistant Pseudomonas Aeruginosa

“…In 2013, we have collected P. aeruginosa strains throughout Cairo, Egypt, and the resistance levels to different antibiotics were determined. Data obtained revealed that the most majority of antibiotics (including carbapenems) have little to moderate effects which could give us a warning of the occurrence of outbreaks caused by this pathogen (Mabrouk et al, 2016). Ongoing a more detailed investigation was urgently needed.…”

Evaluation of Polymyxcin B in Combination With Ciprofloxacin Antibiotics Against Pseudomonas Aeuruginosa

“…Reported effect of Ciprofloxacin against P. aeruginosa appeared to be damage to the peptidoglycan layer of the cell envelope. Various concentrations of Ciprofloxacin had already been shown to enhance bacterial uptake of antibacterial and to enhance antibacterial activity Polymyxin is known to damage cell envelope structures of both stationary and dividing cells (Newton 1954), and would be expected to have a similar [18], [19].…”