Prevalence and Toxin Characteristics of Microorganism on Hand Towels Using for Children in Child Care Center

Kim, Jung-Beom; Kim, Nan-Yong; Kang, Suk-Ho; Do, Young-Sook; Eom, Mi-Na; Yoon, Mi‐Hye; Lee, Jong-Bok

doi:10.13103/jfhs.2013.28.2.138

Cited by 3 publications

(1 citation statement)

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“…B. cereus toxin gene PCR was performed with each toxin gene primer (hblA, hblC, hblD, entFM, nheA, nheB, nheC, and cytK ) (Kim et al, 2013). PCR reaction conditions were as follows: pre-denaturation at 94℃ for 2 min, denaturation at 94℃ for 60 s, annealing (hblA 56℃, hblC 58℃, hblD 58℃, entFM 72℃, nheA 56℃, nheB 54℃, nheC 54℃, cytK 48℃), 60 s, and final extension at 72℃ for 5 min.…”

Section: Pcr Reactionmentioning

confidence: 99%

Toxin gene and antibiotic resistance of Staphylococcus aureus and Bacillus cereus isolated from kitchen cleaning tools in child care centers

Kim¹,

Im²,

Kim³

et al. 2022

Korean J. Food Preserv.

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This study analyzed the toxin genes and antibiotic resistance of foodpoisoning bacteria isolated from dishcloths and scourers in child care centers. Two S. aureus and five B. cereus strains isolated from dishcloths and scourers, respectively, were used in this study. The toxin genes of S. aureus and B. cereus were detected using polymerase chain reaction (PCR) methods. Antibiotic resistance was determined using the disc diffusion method according to the Clinical and Laboratory Standard Institute criteria. Toxin gene analysis revealed the presence of sei and seg toxin genes in S. aureus isolated from dishcloths and scourers. All five B. cereus strains possessed the nheA, nheB, nheC, hblA, hblC, and hblD enterotoxin genes that cause diarrhea. The entFM enterotoxin gene was detected in three of the five isolates, but the cytK enterotoxin gene and ces vomiting toxin gene were not detected. S. aureus and B. cereus isolated from kitchen cleaning tools were found to be multidrug-resistant strains that were resistant to five to eight antibiotics. Therefore, thorough sterilization and disinfection of dishcloths and scourers is required to prevent S. aureus and B. cereus from contaminating cooking utensils.Keywords kitchen cleaning tools, child care center, food-borne pathogens, toxin characteristics, antibiotic resistance

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Section: Pcr Reactionmentioning

confidence: 99%