Prevalence and serovar diversity of Salmonella spp. in primary horticultural fruit production environments

Gomba, Annancietar; Chidamba, Lizyben; Korsten, Lisé

doi:10.1016/j.foodcont.2016.04.026

Cited by 21 publications

(10 citation statements)

References 45 publications

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“…Micro‐organisms from all samples ( n = 18) were isolated as previously described by Gomba et al . (), with slight modifications. A 25‐g sample from each vine was placed in a beaker with sterile peptone‐buffered water (PBW) (Biolab, Merck, Johnnesburg, South Africa) supplemented with 0.025% Tween80 (Associated Chemical Enterprises, Johannesburg), sonicated for 5 min in an ultrasonic water bath (Labotec, Johannebsurg) to dislodge microbes from plant surfaces.…”

Section: Methodsmentioning

confidence: 99%

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

et al. 2017

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Section: Methodsmentioning

confidence: 99%

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

et al. 2017

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“…Supernatant from macerated fresh produce samples in 0.1% PBW was used to determine presence of total coliforms, E. coli, Enterococci, and Pseudomonas with Colilert-18, Enterolert- For Salmonella detection, macerated baby spinach, lettuce, and cabbage samples were incubated at 37 8C for 24 hrs. Thereafter, 0.1 ml of the pre-enriched culture was inoculated in 10 ml Rappaport-Vassiliadis (Merck) and incubated at 42 8C for 18-24 hrs before plating (0.1 ml) in Xylose Lysine Deoxycholate agar (Merck) (Gomba et al, 2016). Pre-enriched macerated vegetable samples (0.1 ml) were also enriched to detect the presence of L. monocytogenes using 10 ml Listeria enrichment broth, incubated at 37 8C for 18-24 hrs and then plated on Listeria agar (Merck).…”

Section: Microbiological and Pathogen Analyses 221 | Leafy Green mentioning

confidence: 99%

Assessment of irrigation water quality and microbiological safety of leafy greens in different production systems

Jongman

Korsten

2016

Journal of Food Safety

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Foodborne disease outbreaks associated with fresh produce irrigated with contaminated water are a constant threat to consumer health. In this study, the impact of irrigation water on product safety from different food production systems (commercial to small‐scale faming and homestead gardens) was assessed. Hygiene indicators (total coliforms, Escherichia coli), and selected foodborne pathogens (Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7) of water and leafy green vegetables were analyzed. Microbiological parameters of all irrigation water (except borehole) exceeded maximum limits set by the Department of Water Affairs for safe irrigation water. Microbial parameters for leafy greens ranged from 2.94 to 4.31 log CFU/g (aerobic plate counts) and 1 to 5.27 log MPN/100g (total coliforms and E. coli). Salmonella and E. coli O157:H7 were not detected in all samples tested but L. monocytogenes was present in irrigation water (commercial and small‐scale farm, and homestead gardens). This study highlights the potential riskiness of using polluted water for crop production in different agricultural settings. Practical applications These results show that the microbial quality of surface water is deteriorating in different agricultural settings. This may be a possible preharvest source of contamination of leafy green vegetables, which may then constitute a health risk to consumers. The presence of Listeria monocytogenes along the food supply chain is a potential health risk if the pathogen proliferates leading to high quantities suitable for onset of listeriosis. The absence of Salmonella and Escherichia coli O157:H7 in the samples tested is an encouraging result. Food safety protocols should be extended to the informal sectors and homestead gardens.

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“…For instance, this procedure can be applied to identify Salmonella spp. (104,107) and for the detection of plasmid insertion in E. coli. (108) It has also been used to discriminate wild-type isolates from ampicillin-resistant E. coli.…”

Section: Matric Assisted Laser Desorption Ionization Time Of Flight (mentioning

confidence: 99%

Irrigation water quality and microbial safety of leafy greens in different vegetable production systems: A review

Jongman

Korsten

2017

Food Reviews International

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Access to large sources of quality water for irrigation is fundamental to the hygienic cultivation of fresh produce. However, due to factors such as contamination of water bodies, access to clean uncontaminated water is fast becoming an ever increasing global challenge. The unavailability of quality source water increases the risk of contamination of fresh produce with human pathogenic microorganisms, which may compromise public health. Over the past few years, there has been a decline in the microbiological quality of surface water and other sources used for irrigation. This is mainly due to upstream faecal contamination. Therefore, the assessment and subsequent suitability of alternative water sources for irrigation such as roof harvested rainwater should be considered. Contrasting views regarding the quality of roof harvested rainwater (RHRW) have been published. Pathogens such as Salmonella and Campylobacter species and Listeria monocytogenes have been reported in RHRW. Leafy green vegetables such as cabbage, spinach and lettuce are produced across a wide range of farming systems from 2 regulated formal (commercial farms) to informal (small-scale and homestead gardens) setups. This review will discuss global water challenges associated with irrigation water, microbial quality of source water for irrigation, crop contamination, and pathogen detection and characterization methodologies.

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Prevalence and serovar diversity of Salmonella spp. in primary horticultural fruit production environments

Cited by 21 publications

References 45 publications

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

Assessment of irrigation water quality and microbiological safety of leafy greens in different production systems

Irrigation water quality and microbial safety of leafy greens in different vegetable production systems: A review

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