Prevalence and Molecular Mechanism of Macrolide and Lincosamide Resistance in Staphylococci Isolated from Subclinical Bovine Mastitis in Turkey

Abstract

ABSTRACT. Macrolide and lincosamide (ML) resistance and the related resistance genes of staphylococci were assessed from cases of bovine subclinical mastitis. Of the 104 Staphylococcus aureus and 62 coagulase negative staphylococcus (CoNS) isolates, 26 (25%) and 12 (19.4%) were resistant to ML, respectively. While constitutive ML resistance phenotype accounted for 15.4% (16/104) of S. aureus and 8.1% (5/62) of CoNS, inducible ML resistance phenotype accounted for 2.9% (3/104) of S. aureus and 3.2% (2/62) of CoNS. Among erythromycin-resistant isolates, single or various combination of different resistance genes were detected. The results of this study showed that ML resistance was prevalent among staphylococci from subclinical bovine mastitis cases in Hatay, Turkey. Therefore, a continuous surveillance is necessary to minimise the spread of antimicrobial-resistant staphylococci.KEY WORDS: bovine subclinical mastitis, macrolide-lincosamide resistance, staphylococcus.

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human

“…On the other hand, one isolate was found to be resistant to tilmicosin and the MIC 90 value of tilmicosin was 12 µg/ml. MIC values for the ML group antibiotics were found to be similar to those reported by Aslantaş et al[21] and Li et al[28]. No quinupristin/ dalfopristine resistant strains were detected in the study, and the MIC 90 value of quinupristin/dalfopristine was similar to the MIC 90 values obtained by Li et al[28].…”

“…There have been few studies investigating the iML phenotype in S. aureus isolates obtained from cattle. In these studies, the prevalence of iML resistance in the isolates was determined as very low (0.39-2.9%) [21,40]. However, some researchers indicated the iML phenotype in the isolates was present at a higher rate (52.8%-95%) [28,45].…”

“…Macrolide, lincosamide and streptogramin (MLS) group antibiotics are frequently used in the treatment of infections caused by β-lactam-resistant staphylococcal isolates [21]. High-level of β-lactam resistance has been found in staphylococcal strains in studies conducted in Türkiye [22][23][24].…”

“…However, there are a limited number of studies investigating the MIC values of penicillin, oxacillin (methicillin) and macrolidelincosamide-streptogramin group antimicrobial agents and resistance genes in S. aureus strains isolated from bovine mastitis. In this context, in a study, conducted in Türkiye, investigating both phenotypic and genotypic characterization of ML resistance, it was reported that macrolide-lincosamide (ML) resistance was detected in 25% of S. aureus strains isolated from milk samples of cows with subclinical mastitis [21]. In this study, it was aimed to investigate penicillin, oxacillin, and MLS resistance in S. aureus isolates isolated from bovine mastitis and human by phenotypic and genotypic methods.…”

Antibiotic Resistance in <i>Staphylococcus aureus</i> Strains Isolated from Cows with Mastitis in Eastern Poland and Analysis of Susceptibility of Resistant Strains to Alternative Nonantibiotic Agents: Lysostaphin, Nisin and Polymyxin B

“…Additionally, eighteen of these strains were found to be phenotypically resistant to methicillin [35]. However, Aslantaş et al [1], who analyzed a group of 104 strains isolated from subclinical bovine mastitis cases during 2006 to 2008 in Hatay Province, Turkey, found that 25% of them were resistant to macrolide and lincosamide (ML) antibiotics. Resistance to β-lactam antibiotics, ampicillin (59.5%) and penicillin (61.4%), was very common in a group of over 170 strains isolated in Estonia [14].…”