Prevalence and molecular mechanism of macrolide resistance in β-haemolytic streptococci in The Netherlands

Leer-Buter, Coretta van; Mouton, Johan W.; Klaassen, Corné H. W.; Handgraaf, C.M.A.; Sunnen, S.; Melchers, Willem J. G.; Sturm, Patrick

doi:10.1016/j.ijantimicag.2010.02.010

Cited by 9 publications

(2 citation statements)

References 14 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, the distribution of erm genes depends on the bacterial species. For example, ermA is more characteristic of S. aureus, whereas ermB is more characteristic of beta-haemolytic streptococci ( Roberts 2004 , Buter et al 2010 , Meehan et al 2014 , Vitali et al 2014 ). Moreover, among CoNS, the type of erm gene also depends on the geographical region of their isolation.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

Juda

Chudzik-Rząd

Malm

2016

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Coagulase-negative staphylococci, particularly Staphylococcus epidermidis, can be regarded as potential reservoirs of resistance genes for pathogenic strains, e.g., Staphylococcus aureus. The aim of this study was to assess the prevalence of different resistance phenotypes to macrolide, lincosamide, and streptogramins B (MLSB) antibiotics among erythromycin-resistant S. epidermidis, together with the evaluation of genes promoting the following different types of MLSB resistance:ermA, ermB, ermC,msrA, mphC, and linA/A’. Susceptibility to spiramycin was also examined. Among 75 erythromycin-resistantS. epidermidis isolates, the most frequent phenotypes were macrolides and streptogramins B (MSB) and constitutive MLSB (cMLSB). Moreover, all strains with the cMLSB phenotype and the majority of inducible MLSB (iMLSB) isolates were resistant to spiramycin, whereas strains with the MSB phenotype were sensitive to this antibiotic. The D-shape zone of inhibition around the clindamycin disc near the spiramycin disc was found for some spiramycin-resistant strains with the iMLSB phenotype, suggesting an induction of resistance to clindamycin by this 16-membered macrolide. The most frequently isolated gene was ermC, irrespective of the MLSB resistance phenotype, whereas the most often noted gene combination wasermC, mphC, linA/A’. The results obtained showed that the genes responsible for different mechanisms of MLSB resistance in S. epidermidis generally coexist, often without the phenotypic expression of each of them.

show abstract

Section: Discussionmentioning

confidence: 99%

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

Juda

Chudzik-Rząd

Malm

2016

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…In Europe, the major clones encoding mef (A) were emm4-ST39 and emm75-ST49 and, after the 2000s, mostly emm12-ST36. The erm(B) was associated with emm28-ST52 at first and later it was found in emm11-ST403 lineages, and erm(TR) raised recently together with emm77-ST63 [3,16,17,[34][35][36][39][40][41][42][43][44][45][46][47][49][50][51]. S1 are depicted as yellow (0-25%), orange (25-50%), blue (50-75%) and green (75-100%) zones, and grey zones mean countries with no data.…”

Section: Macrolide Resistance In β-Haemolitic Streptococcusmentioning

confidence: 97%

An Overview of Macrolide Resistance in Streptococci: Prevalence, Mobile Elements and Dynamics

et al. 2022

View full text Add to dashboard Cite

Streptococcal infections are usually treated with beta-lactam antibiotics, but, in case of allergic patients or reduced antibiotic susceptibility, macrolides and fluoroquinolones are the main alternatives. This work focuses on studying macrolide resistance rates, genetic associated determinants and antibiotic consumption data in Spain, Europe and also on a global scale. Macrolide resistance (MR) determinants, such as ribosomal methylases (erm(B), erm(TR), erm(T)) or active antibiotic efflux pumps and ribosomal protectors (mef(A/E)-mrs(D)), are differently distributed worldwide and associated with different clonal lineages and mobile genetic elements. MR rates vary together depending on clonal dynamics and on antibiotic consumption applying selective pressure. Among Streptococcus, higher MR rates are found in the viridans group, Streptococcus pneumoniae and Streptococcus agalactiae, and lower MR rates are described in Streptococcus pyogenes. When considering different geographic areas, higher resistance rates are usually found in East-Asian countries and milder or lower in the US and Europe. Unfortunately, the availability of data varies also between countries; it is scarce in low- and middle- income countries from Africa and South America. Thus, surveillance studies of macrolide resistance rates and the resistance determinants involved should be promoted to complete global knowledge among macrolide resistance dynamics.

show abstract

Are clindamycin and ciprofloxacin appropriate for the empirical treatment of diabetic foot infections?

Vries

Ekkelenkamp

Peters

2013

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

The objective of this study was to determine if specific species of microorganisms are associated with severe infection and amputation in patients with a diabetic foot infection and to evaluate the effectiveness of clindamycin and ciprofloxacin in this population. A retrospective cohort study was performed at the University Medical Center Utrecht, The Netherlands, for the period January 1st 2005 to January 1st 2010. Patients with ICD-9 codes 'diabetic foot' were selected from the hospital database. We evaluated the association of Staphylococcus aureus and Gram-negative species (GNS) with severity of infection, number of amputations, and healing rates. No significant association was found between the different microorganisms and infection severity. Coinfections of GNS and S. aureus were significantly associated with amputation compared to infections with only S. aureus [p = 0.016, odds ratio (OR) 4.9, confidence interval (CI) 1.4-16.9]. The empiric antibiotic regimen of clindamycin and ciprofloxacin covered only 85 % of S. aureus and 78 % of GNS diabetic foot infections. In mild diabetic foot infection in the studied population, where methicillin-sensitive S. aureus and streptococci are the most likely pathogens, a beta-lactam antibiotic such as flucloxacillin would likely be more effective than clindamycin. In moderate and severe infections, where GNS as a causative organism cannot be safely excluded, broad-spectrum antibiotic therapy may be more adequate.

show abstract

Prevalence and molecular mechanism of macrolide resistance in β-haemolytic streptococci in The Netherlands

Cited by 9 publications

References 14 publications

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

The prevalence of genotypes that determine resistance to macrolides, lincosamides, and streptogramins B compared with spiramycin susceptibility among erythromycin-resistant Staphylococcus epidermidis

An Overview of Macrolide Resistance in Streptococci: Prevalence, Mobile Elements and Dynamics

Are clindamycin and ciprofloxacin appropriate for the empirical treatment of diabetic foot infections?

Contact Info

Product

Resources

About