Prevalence and Molecular Epidemiology of Human Papillomavirus Infection in Italian Women with Cervical Cytological Abnormalities

Meloni, Angelo; Pilia, Roberta; Campagna, Marcello; Usai, Antonella; Masia, Giuseppina; Caredda, Valeria; Coppola, Rosa

doi:10.4081/jphr.2014.157

Cited by 22 publications

(30 citation statements)

References 44 publications

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“…HPV16 together with HPV18 are known to be responsible for approximately 70% of the cervical cancer cases worldwide [6, 8, 9]. The prevalence of HPV18 has been reported in other studies, ranging between 1.8% and 16.3% [20, 21, 23, 27]. In agreement with the aforementioned studies, the prevalence of HPV18 in the present study was 4.1%.…”

Section: Discussionsupporting

confidence: 91%

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

2017

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BackgroundHuman papillomavirus (HPV) is the most common sexually transmitted agent, and it can cause cervical lesions and cancer in females. Currently, information regarding the prevalence of HPV in Cyprus is lacking. The aim of this study was to evaluate the HPV type-specific prevalence in 596 women, aged 19–65 years, with cytological abnormalities. Additionally, in a subset of 348 women for whom cytology results of the Pap test were available, the association between HPV infection and cervical disease was investigated.MethodsHPV detection and typing was carried out using PCR and restriction fragment length polymorphism analysis, respectively.ResultsOverall, the HPV prevalence was 72.8%, and it was shown to be age dependent, with a decreasing prevalence until the age of 45 years (p = 0.0018, χ2). Two hundred and fifty-eight women (59.4%) were infected with high-risk HPV, 151 (34.8%) with low-risk HPV, and 25 (5.8%) with HPV types of unknown risk. The most common high-risk HPV type was HPV16 (17.7%), followed by HPV31 (12.9%), HPV58 (7.1%), HPV68 (4.6%), HPV18 (4.1%), and HPV56 (3.7%). Among the women for whom cytology results were available, 268 (77%) were HPV positive, with a sample distribution as follows: 188 (74%) had atypical squamous cells of undetermined significance (ASCUS), 61 (85.9%) had low-grade squamous intraepithelial lesion (L-SIL), and 19 (82.6%) had high-grade squamous intraepithelial lesion (H-SIL). HPV16 was the most common type among women affected by L-SIL (19.7%) and H-SIL (15.8%), with HPV31 being the most common type in women affected by ASCUS (16.5%).ConclusionsThe present study provides the first epidemiological data related to HPV prevalence and type distribution in Cypriot women with cytological abnormalities.

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Section: Discussionsupporting

confidence: 91%

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

2017

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“…Furthermore, participants with ≥1 HR-HPV type were more often diagnosed with HSIL than participants with LR-HPV. Similar observations concerning the association of poor cytological diagnosis with carcinogenic HPV types have been previously reported (36,38). In all grades of cytological diagnosis, HPV16 was the most common HPV type (ASC-US, 29.46%; LSIL, 29.58%; HSIL, 43.22%).…”

Section: Discussionsupporting

confidence: 70%

“…In the present study, an outdated HPV classification system with several probably HR-HPV types identified that the HR-HPV prevalence was 81.50% in participants <30 years of age. This is slightly lower than the prevalence previously described for other regions in Germany (88.40%) (36). However, using a classification system based on novel biological evidence, which revealed that more HPV types exhibit carcinogenicity than previously acknowledged (10,33), the prevalence of HR-HPV types identified by the present study increased to 91.53%.…”

Section: Discussioncontrasting

confidence: 51%

“…In addition, the present study observed that the prevalence of HPV16 and 18 in participants >30 years old was 33.81%, which is close to the prevalence of 34.50% identified in a previous study (36). In participants aged <30 years old, the highest incidence of HPV16 and 18 was 47.62%, which is higher than the prevalence of 37.40% identified in a previous study on young women <30 years of age in Germany (37).…”

Section: Discussioncontrasting

confidence: 50%

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Shift in prevalence of HPV types in cervical cytology specimens in the era of HPV vaccination

Fischer

Bettstetter²,

Becher

et al. 2016

Oncology Letters

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Abstract. The aim of the present population-based cohort study was to analyze the association between the prevalence of 32 types of human papilloma virus (HPV) in 615 female patients with abnormal cervical cytopathology findings. In total, 32 HPV types were screened by DNA array technology. HPV infection was detected in 470 women (76.42%), 419 of whom (89.15%) were infected with ≥1 high-risk (HR)-HPV type. HPV16, which is recognized as the main HR-HPV type responsible for the development of cervical cancer, was observed in 32.98% of HPV + participants, followed by HPV42 (18.09%), HPV31 (17.66%), HPV51 (13.83%), HPV56 (10.00%), HPV53 (8.72%) and HPV66 (8.72%). The prevalence of HR-HPV types, which may be suppressed directly (in the case of HPV16 and 18), or possibly via cross-protection (in the case of HPV31) following vaccination, was considerably lower in participants ≤22 years of age (HPV16, 28.57%; HPV18, 2.04%; HPV31, 6.12%), compared with participants 23-29 years of age (HPV16, 45.71%; HPV18, 7.86%; HPV31, 22.86%), who were less likely to be vaccinated. Consequently, the present study hypothesizes that there may be a continuous shift in the prevalence of HPV types as a result of vaccination. Furthermore, the percentage of non-vaccine HR-HPV types was higher than expected, considering that eight HPV types formerly classified as 'low-risk' or 'probably high-risk' are in fact HR-HPV types. Therefore, it may be important to monitor non-vaccine HPV types in future studies, and an investigation concerning several HR-HPV types as risk factors for the development of cervical cancer is required.

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“…Virtually all cases of cervical and anogenital cancer are caused by approximately 15 hrHPV genotypes [Muñoz et al, ]. HPV 16 is the most common type and is associated with about the 50% of all cases of cervical cancer [Lowy et al, ; Meloni et al, ].…”

Section: Introductionmentioning

confidence: 99%

Higher levels of oxidative DNA damage in cervical cells are correlated with the grade of dysplasia and HPV infection

Visalli

Riso

Facciolà

et al. 2015

Journal of Medical Virology

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The Human papillomavirus is responsible for the most common sexually transmitted infection and is also known to be an oncogenic virus that is associated with cervical, anogenital, and head-neck cancers. The present study aims to assess whether oxidative DNA damage is correlated with the grade of HPV-related lesions. Moreover, we evaluated clinical data and unhealthy lifestyles to verify their possible influence on the genesis of oxidative DNA damage in cervical cells. We quantified the amount of 8-Oxo-2'-deoxyguanosine in DNA as a biomarker of oxidative damage in women with and without HPV infection. We also correlated oxidative damage with different stages of cervical lesions and available clinical data (e.g., HPV genotypes). To identify HPV infections, in which proteins with a transforming potential are produced, we performed a qualitative detection of HPV E6/E7 mRNA. Our results showed greater oxidative damage in HPV-related dysplastic cervical lesions compared to samples with normal cytology, especially in women with high-grade squamous intraepithelial lesions. The latter showed a closed link with high-risk HPV genotypes. Reactive oxygen species can induce DNA double-strand breaks in both the host DNA and in the circular viral episome; this could facilitate the integration of the virus, promoting HPV carcinogenesis. Therefore, in HPV-infected women, it could be useful to reduce additional resources of reactive oxygen/nitrogen species (RONS) with a healthy lifestyle.

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Prevalence and Molecular Epidemiology of Human Papillomavirus Infection in Italian Women with Cervical Cytological Abnormalities

Cited by 22 publications

References 44 publications

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

HPV prevalence and type distribution in Cypriot women with cervical cytological abnormalities

Shift in prevalence of HPV types in cervical cytology specimens in the era of HPV vaccination

Higher levels of oxidative DNA damage in cervical cells are correlated with the grade of dysplasia and HPV infection

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