Prevalence and molecular characterizations of enterovirus D68 among children with acute respiratory infection in China between 2012 and 2014

Xiao, Qiuyan; Luo, Ren; Zheng, Shouyan; Wang, Lili; Xie, Xiaohong; Deng, Yu; Zhao, Yongkun; Zhao, Xiaodong; Luo, Zhengxiu; Fu, Zhou; Huang, Aiping; Liu, Enmei

doi:10.1038/srep16639

Cited by 34 publications

(22 citation statements)

References 33 publications

(64 reference statements)

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“…In particular, EV-D68 outbreaks in the United States in 2014 have raised concerns of a pandemic789. Similarly, between 2006 and 2014 EV-D68 infections in China were also detected in children and adults with respiratory infections410111213. In contrast to the symptoms caused by EV-D68 and the prevalence observed in the United States, none of the cases in China had nervous system dysfunction and no notable outbreak was reported.…”

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confidence: 99%

“…To understand the prevalence of EV-D68 in China, we analyzed 64 Chinese EV-D68 sequences including 54 sequences, which were previously identified410111213, two sequences submitted directly to Genbank, and eight sequences that were detected from children hospitalized in Beijing Children’s Hospital between 2007 and 2014. Our results revealed sequence differences in the 3′-UTR and puff region of VP2 as well as six coding polymorphisms that are associated with neurovirulence7.…”

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confidence: 99%

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Genetic divergence of enterovirus D68 in China and the United States

Zou

Xie

Liu

et al. 2016

Sci Rep

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The largest outbreak of human enterovirus 68 (EV-D68) infections associated with severe respiratory illness and neurological complications emerged from the United States in 2014. China reported the circulation of EV-D68 since 2006, but these cases were sporadic and did not display neurological symptoms. Yet viral determinants responsible for the difference in prevalence between China and the U.S. were not clear. We analyzed the genome of 64 reported Chinese EV-D68 strains and found that genogroup replacement has occurred in China since 2006. The six coding mutations (M291T, V341A, T860N, D927N, S1108G and R2005K) associated with neurovirulence reported in American strains were not found in Chinese strains. Moreover, 2014 Chinese strains had a unique R220A mutation in the puff region of VP2 while R220E mutation occurred in other strains. Like other enteroviruses, the loop sequences of the domain X and Y in the 3′-UTR of the Chinese strains are complementary. However, the X loop sequences of the 2014 American strains were not complementary but identical to Y loop sequences. These results indicate that different EV-D68 strains circulated in China and America and the mutations might be responsible for different prevalence. Our findings also provide new evidence for the sequence diversity of EV-D68.

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confidence: 99%

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confidence: 99%

Genetic divergence of enterovirus D68 in China and the United States

Zou

Xie

Liu

et al. 2016

Sci Rep

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“…Therefore, understanding the status of neutralizing antibodies (nAbs) against EV-D68 in population is important to assess the prevalence of previous infection and transmission potential of EV-D68. Unfortunately, only a few serological investigations have been conducted in a small part of China (including Beijing [21,22], Jiangsu [18], Chongqing [23] and Taiwan [24]), as well as in the United States [25], the United Kingdom [26] and the Netherlands [27], the pattern of immune responses have not been well studied for EV-D68. Xiamen is a major seaport and tourist city along the southeast coast of China with an increased oating population.…”

Section: Introductionmentioning

confidence: 99%

Serological Study of Neutralizing Antibodies Against Enterovirus D68 Among Healthy Population in Xiamen, China

Cheng¹,

Lin²,

Zhu³

et al. 2020

Preprint

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Abstract Background: Enterovirus D68 (EV-D68) infections poses a serious public health threat, which caused outbreaks of severe respiratory illness and has been closely associated with "polio-like" central nervous system disease. The patterns of immune responses to EV-D68 have not been fully understood. This study was performed to investigate the level of neutralizing antibody against EV-D68 among the healthy population in Xiamen City.Methods: A total of 515 serum samples from healthy people were selected by stratified random sampling in Xiamen City in 2016, and the titer of neutralizing antibody against EV-D68 was detected by an efficient micro-neutralization assay.Results: The overall seroprevalence of neutralizing antibody against EV-D68 was 81.9%, and the overall geometric mean titers (GMT) was 248.0. The positive rates were 42.2%, 49.3%, 71.9%, 94.2% and 98.5%, respectively, in groups of <1, 1-3, 4-6, 7-19 and 20-39 years old, and were up to 100% in the 40-59 and ≥ 60 age groups. The GMTs in these seven groups were 1:39.8, 1:80.6, 1:133.2, 1:283.7, 1:253.3, 1:308.1 and 1:405.0, respectively. The seroprevalence and GMT of EV-D68 showed an increased trend with age, while gender and geographic area didn’t show significant impact.Conclusions: EV-D68 infection was prevalent in Xiamen City. Children are the high-risk groups for EV-D68-related illnesses, especially those aged ≤ 3 years. It is necessary to establish preventive measures and strengthened the surveillance on EV-D68 to prevent disease outbreaks.

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“…Además, la infección se asoció con linfocitopenia y propensión al desarrollo de hipoxemia 10 . Debido al incremento de los casos de EV-D68 en todo el mundo 3,[11][12][13][14][15] , se le considera como un patógeno reemergente 1 .…”

Section: Introductionunclassified