Prevalence and molecular characterization of Cryptosporidium and Giardia in pre-weaned native calves in the Republic of Korea

Ryu, Jae Hyoung; Shin, Seung-Uk; Choi, Kwang Shik

doi:10.1007/s00436-019-06482-9

Cited by 23 publications

(31 citation statements)

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“…parvum was detected only in calves aged ≤20 days, and the infection rate was the highest in calves aged 11−20 days. This observation is consistent with a previous study performed by our group [ 18 ]. According to our findings, C .…”

Section: Discussionsupporting

confidence: 94%

“…To date, three C . parvum subtypes have been detected in calves in the KOR [ 18 , 24 ], whereas one subtype (IIaA16G3R1) was not found in this study. In addition to the two subtypes (IIaA15G2R1 and IIaA18G3R1) described above, nine other subtypes (IIaA14G1R1, IIaA14G3R1, IIaA15G1R1, IIaA16G4R1, IIaA17G3R1, IIaA17G4R1, IIaA19G1R1, IIaA19G3R1, and IIaA19G4R1) that have not previously been detected in the KOR were identified for the first time, showing the presence of high genetic diversity.…”

Section: Discussioncontrasting

confidence: 76%

“…IIaA15G2R1 has been known as the most prevalent C . parvum subtype infecting humans and cattle in many countries [ 7 , 34 , 55 – 59 ] and has also been detected in calves without diarrhea [ 18 , 33 , 60 ]. There seems to be no relationship between the subtype and diarrhea.…”

Section: Discussionmentioning

confidence: 99%

“…parvum based on sequence analysis of the 60-kDa glycoprotein ( gp 60) gene, Ⅱa and Ⅱd subtypes have been detected in both humans and calves and can cause zoonotic cryptosporidiosis [ 17 ]. The Ⅱa subtype is mostly identified in calves, and IIaA15G2R1 is the predominant subtype [ 7 ] globally, including the Republic of Korea (KOR) [ 18 ]. The IId subtype is usually found in lambs and goat kids [ 4 , 19 ] and has been described in calves in some countries such as Sweden, Turkey, Egypt, and China [ 20 – 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and distribution pattern of Cryptosporidium spp. among pre-weaned diarrheic calves in the Republic of Korea

et al. 2021

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Cryptosporidium spp. are protozoan parasites that belong to subphylum apicomplexa and cause diarrhea in humans and animals worldwide. Data on the prevalence of Cryptosporidium spp. and its subtypes among calves in the Republic of Korea (KOR) are sparse. Hence, our study aimed to investigate the prevalence and association between the age of calf and the identified Cryptosporidium spp. and to determine the genotypes/subtypes of Cryptosporidium spp. in pre-weaned calves with diarrhea in the KOR. A total of 460 diarrheic fecal samples were collected from calves aged 1−60 days and screened for Cryptosporidium spp. by the 18S rRNA gene. Species identification was determined using the sequencing analysis of the 18S rRNA gene, and C. parvum-positive samples were subtyped via the sequence analysis of the 60-kDa glycoprotein (gp60) gene. Sequence analysis based on the 18S rRNA gene revealed the presence of three Cryptosporidium spp., namely, C. parvum (n = 72), C. ryanae (n = 12), and C. bovis (n = 2). Co-infection by these species was not observed. The infection rate was the highest in calves aged 11−20 days (26.1%, 95% CI 17.1−35.1), whereas the lowest rate was observed in calves aged 21−30 days (7.7%, 95% CI 0.0−16.1). The prevalence of C. parvum was detected exclusively in calves aged ≤20 days, and the highest infection rate of C. ryanae was seen in calves ≥31 days of age. The occurrence of C. parvum (χ2 = 25.300, P = 0.000) and C. ryanae (χ2 = 18.020, P = 0.001) was significantly associated with the age of the calves. Eleven different subtypes of the IIa family that belonging to C. parvum were recognized via the sequence analyses of the gp60 gene. Except for two (IIaA18G3R1 and IIaA15G2R1) subtypes, nine subtypes were first identified in calves with diarrhea in the KOR. IIaA18G3R1 was the most frequently detected subtype (72.2% of calves), followed by IIaA17G3R1 (5.6%), IIaA15G2R1 (4.2%), IIaA19G4R1 (4.2%), IIaA16G4R1 (2.8%), IIaA17G4R1 (2.8%), IIaA19G3R (2.8%), IIaA14G1R1 (1.4%), IIaA14G3R1 (1.4%), IIaA15G1R1 (1.4%), and IIaA19G1R1 (1.4%) These results suggest that the prevalence of Cryptosporidium spp. is significantly associated with calf age. Furthermore, the findings demonstrate the high genetic diversity of C. parvum and the widespread occurrence of zoonotic C. parvum in pre-weaned calves. Hence, calves are a potential source of zoonotic transmission with considerable public health implications.

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Section: Discussionsupporting

confidence: 94%

Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and distribution pattern of Cryptosporidium spp. among pre-weaned diarrheic calves in the Republic of Korea

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“…According to the subtyping of C. parvum based on sequence analysis of the 60-kDa glycoprotein (gp60) gene, Ⅱa and Ⅱd subtypes have been detected in both humans and calves and can cause zoonotic cryptosporidiosis [17]. The Ⅱa subtype is mostly identified in calves, and IIaA15G2R1 is the predominant subtype [7] globally, including the Republic of Korea (KOR) [18]. The IId subtype is usually found in lambs and goat kids [4,19] and has been described in calves in some countries such as Sweden, Turkey, Egypt, and China [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Molecular prevalence and genotyping of Giardia duodenalis in cattle in Central Anatolia Region of Turkey

et al. 2020

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Prevalence and molecular characterization of Cryptosporidium and Giardia in pre-weaned native calves in the Republic of Korea

Cited by 23 publications

References 50 publications

Prevalence and distribution pattern of Cryptosporidium spp. among pre-weaned diarrheic calves in the Republic of Korea

Prevalence and distribution pattern of Cryptosporidium spp. among pre-weaned diarrheic calves in the Republic of Korea

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Molecular prevalence and genotyping of Giardia duodenalis in cattle in Central Anatolia Region of Turkey

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