Prevalence and molecular characterization of Staphylococcus aureus in commercially available meat over a one-year period in Iowa, USA

Thapaliya, Dipendra; Forshey, Brett M.; Kadariya, Jhalka; Quick, Megan K.; Farina, Sarah A.; Brien, Ashley O’; Nair, Rajeshwari; Nworie, Amos; Hanson, Blake; Kates, Ashley E.; Wardyn, Shylo E.; Smith, Tara C.

doi:10.1016/j.fm.2017.01.015

Cited by 58 publications

(40 citation statements)

References 57 publications

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“…However, MRSA has been also found associated with community setting (CA-MRSA) and with livestock (LA-MRSA) (Pantosti 2012). Recently, MRSA has been isolated in various types of food products, including raw milk, dairy products (Carfora et al 2015;Caruso et al 2016;Parisi et al 2016;Basanisi et al 2017;Giaciniti et al 2017;Titouche et al 2019;Papadopoulos et al 2019a) and meat (Tang et al 2017;Thapaliya et al 2017). Transmission of zoonotic MRSA to humans can occur via either animal contact or contaminated food (Oniciuc et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

Titouche

Houali

Ruiz-Ripa

et al. 2020

J. Appl. Microbiol.

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Aims The aim of this study was to characterize Staphylococcusaureus isolates of food origin (dairy and meat products, pastries and sandwiches) determining the carriage in enterotoxin genes and the antimicrobial resistance pheno/genotypes. Methods and results A total of 300 food samples were collected and analysed for the detection of S. aureus. The presence of enterotoxin genes was investigated by multiplex PCRs. Resistance of isolates to 11 antimicrobials was determined using disc diffusion method and molecular characterization of methicillin‐resistant S. aureus was carried out by spa typing and multilocus sequence typing. Overall, 51 out of 300 samples (17%) were contaminated with S. aureus, and 104 isolates were recovered. In all, 65 of these isolates (62·5%) harboured one or more genes encoding for staphylococcal enterotoxins, being seg and sei the most observed genes. The highest resistance profile was ascribed to penicillin G (95·19%). Five isolates were methicillin‐resistant (MRSA) harbouring the mecA gene. All MRSA isolates belonged to the sequence type ST5 and to two different spa types (t450 and t688); the MRSA‐t450 isolate carried the scn gene (specific marker of the immune evasion cluster system), but the four MRSA‐t688 isolates were scn negative. The MRSA isolates carried enterotoxin genes but were negative for the genes of the Panton Valentine leukocidine (lukF/S‐PV). Conclusion The presence of enterotoxigenic S. aureus isolates, including MRSA, in food samples can represent a risk for public health. Significance and Impact of this Study This work describes the molecular characteristics of MRSA strains isolated from foods in Algeria and it can contribute to an extended database concerning the S. aureus isolated from food origin.

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Section: Introductionmentioning

confidence: 99%

Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

Titouche

Houali

Ruiz-Ripa

et al. 2020

J. Appl. Microbiol.

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“…These combined effects result in reduced weight of intestinal tissue and turnover of mucosal cells (Visek 1978), leading to increased carcass body weight gain (Wang et al 2016;Attia et al 2011). However, antibiotics fed to chickens may cause resistance and cross resistance to antibiotics in pathogenic bacteria (Thapaliya et al 2017), and for E. coli at least, in one study with limited sample size, there was a strong indication that transmission of resistance from poultry to humans had occurred (van den Bogaard et al 2001). Despite this, there are differences of opinion about whether the emerging antibiotic resistance is largely due to overuse in the human community (Cervantes 2015).…”

Section: Introductionmentioning

confidence: 99%

A comparison of the effects of antibiotics, probiotics, synbiotics and prebiotics on the performance and carcass characteristics of broilers

et al. 2018

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Routine use of the antibiotic flavomycin in broiler production may lead to resistance, and alternative growth promoters are used to enhance performance. Two hundred day-old male Ross 308 broiler chicks were allocated to five dietary supplements included from d 1-42: flavomycin, three possible alternatives, a probiotic, prebiotic and a synbiotic, as well as a control treatment. There were four replicate cages of 10 birds each in each treatment. Compared with the control and antibiotics treatments, the probiotic, prebiotic and synbiotic treatments increased (p = 0.001) weight gain (64, 66, 73, 70 and 74 g/d, respectively). The synbiotic treatment reduced (p = 0.004) the feed conversion ratio, compared with the control and antibiotic treatments (1.70, 1.84, 1.83, respectively). Compared with the control and antibiotic treatments, the birds fed the synbiotic treatment had greater relative gizzard (+47%) and spleen weights (+115%), and lighter kidneys (-47%). The birds fed the symbiotic treatment also had thinner walls of the caudal gut segments. The prebiotic had the most beneficial effect on cecal microbiota, stimulating aerobic and lactic acid producing bacteria and reducing Escherichia coli bacteria. Enterococci were increased in the antibiotic treatment. We conclude that there were significant performance and health benefits of using prebiotics, probiotics and synbiotics for broilers, rather than antibiotics.

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“…With such a model, the mean prevalence at any sampled site (breast, carcass, thigh or wings) was estimated as 0.0135 (95% CI: 0.0015 -0.0270) for Salmonella, 0.0211 (95% CI: 0.0004 -0.0563) for L. monocytogenes and 0.0236 (95% CI: 0.0004 -0.0512) for S. aureus. Significantly higher prevalence levels were reported by the EFSA Zoonoses Report; for Salmonella (6.45%) and by Akbar and Anal (2013) and Thapaliya et al (2017) for multidrug resistant S. aureus strains in poultry meat. Differences in prevalence with other studies can be attributed to the number of samples collected in our study and deviations in the analytical methodologies used for pathogen detection in comparison with other works which can yield underestimated prevalence levels (Reiter et al, 2007).…”

Section: Adequacy Of Zero-inflated Binomial (Zib) Regression Modelsmentioning

confidence: 69%

Zero-inflated binomial regressions for modelling low prevalence of pathogens in chicken meat as affected by sampling site

Hernández

Rodrı́guez-Lázaro

Valero

et al. 2018

Microbial Risk Analysis

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Contamination of raw poultry meat with foodborne pathogens could occur because of improper handling at primary production and slaughterhouse levels. Low microbial prevalence data often consists of a high amount of non-detections (zero positives), so a flexible framework is required to characterise the underlying microbial distribution and conduct reliable inferential statistics. Thus, the objective of this work was to evaluate the performance of zero-inflated binomial (ZIB) regression models to describe the effects of sampling site (carcass, thigh, breast, wings) on the measured incidences of Salmonella, Listeria monocytogenes and Staphylococcus aureus on chicken meat. For each pathogen, four regression models based on the zero-inflated binomial ZIB (p, w 0) distribution were fitted to the presence/absence data with sampling site as covariate and random-effects due to sampling occasion either in the binomial probability (p) or in the extra-proportion of non-detections (w 0). For the three pathogens, the sampling site exerted a greater effect on w 0 than on p itself, with breast bearing the lowest prevalence estimates of Salmonella spp. (mean: 0.88%; 95% CI: 0.02-1.95%) and S. aureus (mean 1.48%; 95% CI: 0.01-4.00%). The fitting capacity of the models was further improved when random effects due to sampling occasion were placed in w 0 (deviances decreased from 146.7-156.7 to 140.2-140.6). This would imply that, theoretically, the variability in pathogens' occurrence from batch to batch mainly arises from the variability in non-contaminated zones. At any sampling site, the mean prevalence was estimated as 1.35 (95% CI: 0.15-2.70) for Salmonella, 2.11 (95% CI: 0.04-5.63) for L. monocytogenes and 2.36 (95% CI: 0.04-5.12) for S. aureus. Sampling performance analysis showed that wings were mostly suitable to detect Salmonella and S. aureus with higher probability (0.016 and 0.035 respectively), while for L. monocytogenes, sampling of thigh could be more effective (0.032).

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Prevalence and molecular characterization of Staphylococcus aureus in commercially available meat over a one-year period in Iowa, USA

Cited by 58 publications

References 57 publications

Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

Enterotoxin genes and antimicrobial resistance in Staphylococcus aureus isolated from food products in Algeria

A comparison of the effects of antibiotics, probiotics, synbiotics and prebiotics on the performance and carcass characteristics of broilers

Zero-inflated binomial regressions for modelling low prevalence of pathogens in chicken meat as affected by sampling site

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