Prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL) and plasmidic AmpC β-lactamase (pAmpC) producing &lt;i&gt;Escherichia coli&lt;/i&gt; in dogs

Aslantaş, Özkan; Yılmaz, Ebru Şebnem

doi:10.1292/jvms.16-0432

Cited by 34 publications

(25 citation statements)

References 48 publications

(64 reference statements)

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“…Notwithstanding, the O128 serogroup identified in BProcessing isolates from hides have been linked to serious human infections [97] and may serve as a risk of product contamination during processing. The majority of the O25:ST131 clones from human clinical isolates were CTX-M 15 producers, similar to findings of Aslantaş and Yilmaz [100], who characterized ESBL-EC from Canadian dogs. The specific differences between serogroups along the continuum aligns with MLST and phylogenetic differences in this study and further suggests little to no transmission between cattle and humans.…”

Section: Insights From E Coli Phylogenetics Mlst and Serogroupssupporting

confidence: 85%

Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing Escherichia coli along Segments of the One Health Continuum

Adator

Walker²,

Narváez‐Bravo

et al. 2020

Microorganisms

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Antimicrobial resistance (AMR) has important implications for the continued use of antibiotics to control infectious diseases in both beef cattle and humans. AMR along the One Health continuum of the beef production system is largely unknown. Here, whole genomes of presumptive extended-spectrum β-lactamase E. coli (ESBL-EC) from cattle feces (n = 40), feedlot catch basins (n = 42), surrounding streams (n = 21), a beef processing plant (n = 4), municipal sewage (n = 30), and clinical patients (n = 25) are described. ESBL-EC were isolated from ceftriaxone selective plates and subcultured on ampicillin selective plates. Agreement of genotype-phenotype prediction of AMR ranged from 93.2% for ampicillin to 100% for neomycin, trimethoprim/sulfamethoxazole, and enrofloxacin resistance. Overall, β-lactam (100%; blaEC, blaTEM-1, blaSHV, blaOXA, blaCTX-M-), tetracycline (90.1%; tet(A), tet(B)) and folate synthesis (sul2) antimicrobial resistance genes (ARGs) were most prevalent. The ARGs tet(C), tet(M), tet(32), blaCTX-M-1, blaCTX-M-14, blaOXA-1, dfrA18, dfrA19, catB3, and catB4 were exclusive to human sources, while blaTEM-150, blaSHV-11–12, dfrA12, cmlA1, and cmlA5 were exclusive to beef cattle sources. Frequently encountered virulence factors across all sources included adhesion and type II and III secretion systems, while IncFIB(AP001918) and IncFII plasmids were also common. Specificity and prevalence of ARGs between cattle-sourced and human-sourced presumptive ESBL-EC likely reflect differences in antimicrobial use in cattle and humans. Comparative genomics revealed phylogenetically distinct clusters for isolates from human vs. cattle sources, implying that human infections caused by ESBL-EC in this region might not originate from beef production sources.

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Section: Insights From E Coli Phylogenetics Mlst and Serogroupssupporting

confidence: 85%

Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing Escherichia coli along Segments of the One Health Continuum

Adator

Walker²,

Narváez‐Bravo

et al. 2020

Microorganisms

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“…In this report, ST131/B2 E. coli positive for CTX-M-15 were detected. This clone is a human pandemic one that can possibly be transmitted to humans via direct or indirect contact with companion animals (Aslantas and Yilmaz, 2017).…”

Section: Distribution Of Multi-drug Resistant Organisms In Animalsmentioning

confidence: 99%

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

et al. 2019

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In the last decade, extended-spectrum cephalosporin and carbapenem resistant Gram-negative bacilli (GNB) have been extensively reported in the literature as being disseminated in humans but also in animals and the environment. These resistant organisms often cause treatment challenges due to their wide spectrum of antibiotic resistance. With the emergence of colistin resistance in animals and its subsequent detection in humans, the situation has worsened. Several studies reported the transmission of resistant organisms from animals to humans. Studies from the middle east highlight the spread of resistant organisms in hospitals and to a lesser extent in livestock and the environment. In view of the recent socio-economical conflicts that these countries are facing in addition to the constant population mobilization; we attempt in this review to highlight the gaps of the prevalence of resistance, antibiotic consumption reports, infection control measures and other risk factors contributing in particular to the spread of resistance in these countries. In hospitals, carbapenemases producers appear to be dominant. In contrast, extended spectrum beta lactamases (ESBL) and colistin resistance are becoming a serious problem in animals. This is mainly due to the continuous use of colistin in veterinary medicine even though it is now abandoned in the human sphere. In the environment, despite the small number of reports, ESBL and carbapenemases producers were both detected. This highlights the importance of the latter as a bridge between humans and animals in the transmission chain. In this review, we note that in the majority of the Middle Eastern area, little is known about the level of antibiotic consumption especially in the community and animal farms. Furthermore, some countries are currently facing issues with immigrants, poverty and poor living conditions which has been imposed by the civil war crisis. This all greatly facilitates the dissemination of resistance in all environments. In the one health concept, this work re-emphasizes the need to have global intervention measures to avoid dissemination of antibiotic resistance in humans, animals and the environment in Middle Eastern countries.

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“…While an assessment of the frequency of carbapenem usage in companion animals has not been reported, pharmacokinetic studies have been performed, and companion animal dosage recommendations for imipenem and meropenem are available in veterinary medical textbooks (6)(7)(8). Moreover, extended-spectrum ␤-lactamase (ESBL) and AmpC ␤-lactamase-mediated resistance among Enterobacteriaceae isolates obtained from dogs and cats is common, indicating a potential justification for carbapenem administration (9)(10)(11)(12)(13)(14).…”

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confidence: 99%

Enterobacter cloacae Complex Sequence Type 171 Isolates Expressing KPC-4 Carbapenemase Recovered from Canine Patients in Ohio

Daniels

Chen

Grooters

et al. 2018

Antimicrob Agents Chemother

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Companion animals are likely relevant in the transmission of antimicrobial-resistant bacteria. Enterobacter xiangfangensis sequence type 171 (ST171), a clone that has been implicated in clusters of infections in humans, was isolated from two dogs with clinical disease in Ohio. The canine isolates contained IncHI2 plasmids encoding bla . Whole-genome sequencing was used to put the canine isolates in phylogenetic context with available human ST171 sequences, as well as to characterize their bla plasmids. Citation Daniels JB, Chen L, Grooters SV, Mollenkopf DF, Mathys DA, Pancholi P, Kreiswirth BN, Wittum TE. 2018. Enterobacter cloacae complex sequence type 171 isolates expressing KPC-4 carbapenemase recovered from canine patients in Ohio. Antimicrob Agents Chemother 62:e01161-18. https://doi .

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Prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL) and plasmidic AmpC β-lactamase (pAmpC) producing <i>Escherichia coli</i> in dogs

Cited by 34 publications

References 48 publications

Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing Escherichia coli along Segments of the One Health Continuum

Whole Genome Sequencing Differentiates Presumptive Extended Spectrum Beta-Lactamase Producing Escherichia coli along Segments of the One Health Continuum

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

Enterobacter cloacae Complex Sequence Type 171 Isolates Expressing KPC-4 Carbapenemase Recovered from Canine Patients in Ohio

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