Prevalence and molecular characteristics of sequence type 131 clone among clinical uropathogenic Escherichia coli isolates in Riyadh, Saudi Arabia

Alqasim, Abdulaziz; Jaffal, Ahmad Abu; Alyousef, Abdullah A.

doi:10.1016/j.sjbs.2019.09.020

Cited by 23 publications

(30 citation statements)

References 38 publications

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“…Moreover, multidrug resistance (MDR) was demonstrated in 9 out of 17 E. coli ST131 isolates (52.9%). Several studies were in matching line with the present study [ 13 , 19 , 27 , 28 , 37 ]. This may be due to the fact that E. coli pathogens, particularly, E. coli ST131 clone, have developed resistance to every class of antibiotics introduced to treat human and animal infections [ 41 ], and these infections are particularly challenging to treat [ 17 ].…”

Section: Discussionsupporting

confidence: 91%

“…Thus, rapid initiation of appropriate empirical treatment requires a good knowledge of epidemiological data concerning the sensitivity of uropathogens to antibacterial agents [ 34 ]. In this investigation, the highest rate of resistance in E. coli ST131 isolates was against β -lactams, tetracycline, trimethoprim-sulfamethoxazole, nalidixic acid, and ciprofloxacin which was comparable with the results of several studies around the globe [ 3 , 17 , 19 , 27 , 32 , 35 , 36 ], and this may be due to several reasons including inexpensive antimicrobials, indiscriminate antibiotic usage without medical prescription, and use of antibiotics for a nonoptimal duration. In addition, plasmids harboring resistance determinants can be transferred between bacteria, even between species, leading to the acquisition of resistance to new antibiotics via the emergence of mutant strains [ 4 ].…”

Section: Discussionsupporting

confidence: 85%

“…This high dissemination may be due to several reasons, the most important one is the epidemic potential, and virulence and multidrug resistance ability, as well as its predominance in the human gut, make them rapidly disseminated worldwide, with a high capability of causing extraintestinal infections, particularly UTIs. So that, the dominance of this clone was higher among EXPEC [ 2 , 25 – 27 ]. Furthermore, diverse modes of transmission of resistance, probably with a major role of plasmids, encourages the worldwide spread of this clone [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

Al-Guranie

Al-Mayahie

2020

International Journal of Microbiology

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The emergence of Escherichia coli sequence type 131 (E. coli ST131) clone represents a major challenge to public health globally, since this clone is reported as highly virulent and multidrug-resistant, thus making it successfully disseminated worldwide. In Iraq, there is no previous study dealing with this important clone, so this project was suggested to investigate its presence within uropathogenic E. coli (UPEC) from Iraqi patients in Wasit Province. A total of 112 UPEC isolates from cases of acute urinary tract infection (UTI) were analysed for phylogenetic groups by quadruplex PCR; then, these isolates were investigated for E. coli ST131 clone by both conventional and real-time PCR procedures. The antibiotic susceptibility test was performed by the disk diffusion method. The results revealed that, out of 112 UPEC isolates, 38 (33.9%) belonged to phylogroup B2. For conventional PCR, 92.1% (35/38) of B2 E. coli isolates were positive for E. coli ST131, of which 34 were O25b-ST131 strain and 1 was O16-ST131 strain. However, serogroups O25b and O16 represented 17.1% and 2.8%, respectively. By RT-PCR assay, 15.1% (17/112) and 44.7% (17/38) of total and B2 E. coli isolates were confirmed as being E. coli ST131, respectively. The highest resistance rates of E. coli ST131 isolates were against the β-lactams, while low resistance rates were against amikacin, nitrofurantoin, and gentamicin. Fortunately, all isolates were susceptible to carbapenems. Moreover, 52.9% (9 out of 17) of E. coli ST131 isolates were MDR. In conclusion, the presence of E. coli ST131 among UPEC isolates from Iraqi patients is confirmed with high resistance to most antimicrobials included in this study.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

Al-Guranie

Al-Mayahie

2020

International Journal of Microbiology

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“…Also, all tested resistance genes (bla −CTX-M-15 , bla −oxa-2 , and bla −oxa-10 ) were found to be more prevalent among E. coli isolates obtained from urine and blood. 70 OXA-type βlactamases have high hydrolytic activity against oxacillin and cloxacillin but they are poorly inhibited by clavulanic acid. OXA-2 and OXA-10 have recently reported to have extended hydrolytic spectrum to include oxyimino cephalosporins.…”

Section: Discussionmentioning

confidence: 99%

<p>Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic <em>E. coli</em> Isolated from Egyptian Patients with Different Clinical Infections</p>

El-Baky¹,

Ibrahim²,

Mohamed³

et al. 2020

IDR

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Introduction: Escherichia (E.) coli can cause intestinal and extra-intestinal infections which ranged from mild to life-threatening infections. The severity of infection is a product of many factors including virulence properties and antimicrobial resistance. Objectives: To determine the antibiotic resistance pattern, the distribution of virulence factors and their association with one another and with some selected resistance genes. Methods: Virulence properties were analyzed phenotypically while antimicrobial susceptibility was tested by Kirby-Bauer agar disc diffusion method. In addition, 64 E. coli isolates were tested for 6 colicin genes, fimH, hlyA, traT, csgA, crl virulence genes and bla −CTX-M-15 , bla −oxa-2 , and bla −oxa-10 resistance genes by polymerase chain reaction (PCR). Results: Extra-intestinal pathogenic E. coli isolated from urine and blood samples represented a battery of virulence factors and resistance genes with a great ability to produce biofilm. Also, a significant association (P<0.05) among most of the tested colicin, virulence and resistance genes was observed. The observed associations indicate the importance and contribution of the tested factors in the establishment and the progress of infection especially with Extra-intestinal E. coli (ExPEC) which is considered a great challenging health problem. Conclusion: There is a need for studying how to control these factors to decrease the rate and the severity of infections. The relationship between virulence factors and resistance genes is complex and needs more studies that should be specific for each area.

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“…Burning sensation while urinating and severe pain are the main symptoms of a lower urinary tract infections. Additionally, felling of needing to urinate while the bladder being empty and recurrent urination are indications ( Alqasim et al, 2019 ). These symptoms could be mild to severe.…”

Section: Introductionmentioning

confidence: 99%

Vitamin D deficiency as a risk factor for urinary tract infection in women at reproductive age

Ali

Perdawood

Abdulrahman

et al. 2020

Saudi Journal of Biological Sciences

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Vitamin D deficiency is a pandemic problem and an ever-increasing issue in human nutrition and health. Vitamin D (serum 25-hydroxyvitamin D) deficiency causes many health problems such as autoimmune diseases, Crohn’s disease, diabetes, inflammation, asthma, hypertension, and cancer. Vitamin D3 (cholecalciferol) deficiency has been documented as a persistent problem among adults, children, and elderly persons in most of the countries. Our main objective of this study was to determine the hypothesis that the vitamin D deficiency among women can lead to them developing frequent urinary tract infections. Vitamin D has a potential role in immune regulation and it prevents infections especially urinary tract infections (UTI). Therefore it has positive regulatory role in both acute and recurrent infections especially in women of reproductive ages. As women at this age group have specific differences in their urinary tract and the reproductive organ anatomy, make them more prone for micro-organisms' invasion, The present study was carried out to ascertain certain relation between serum 25-hydroxyvitamin D levels and UTI in women while contemplating the significance of knowing the risk factors associated with UTI and also finding ways to avoid serious complications. 75 women with (case group) UTI were differentiated with 35 healthy with no UTI (control group) and 40 women with UTI and their serum 25-hydroxyvitamin D levels were checked in a case control study. The women were between at 17–52 years of age. Using ELISA, Serum 25-hydroxyvitamin D levels were measured. Analysis and comparison of the results were done among the two groups. Vitamin D mean levels in the case group was considerably lower when in comparison with the control group (11.09 ± 7.571 ng/mL vs. 24.08 ± 11.95 ng/mL, P < 0.001).

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Prevalence and molecular characteristics of sequence type 131 clone among clinical uropathogenic Escherichia coli isolates in Riyadh, Saudi Arabia

Cited by 23 publications

References 38 publications

Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

Prevalence of E. coli ST131 among Uropathogenic E. coli Isolates from Iraqi Patients in Wasit Province, Iraq

<p>Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic <em>E. coli</em> Isolated from Egyptian Patients with Different Clinical Infections</p>

Vitamin D deficiency as a risk factor for urinary tract infection in women at reproductive age

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