Prevalence and molecular characterisation of Cryptosporidium and Giardia in lambs and goat kids in Belgium

Geurden, Thomas; Thomas, Pieter; Casaert, Stijn; Vercruysse, Jozef; Claerebout, Edwin

doi:10.1016/j.vetpar.2008.05.002

Cited by 148 publications

(156 citation statements)

References 14 publications

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“…A c c e p t e d M a n u s c r i p t 11 weeks in Belgium (Geurden et al 2008). It has been suggested that the cervid genotype could possibly emerge as an important human pathogen because current evidence suggests this genotype has a wide host range and zoonotic potential (Santin and Fayer, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, in Spain, C. parvum was the only species detected from 137 microscopypositive diarrheic lambs (aged up to 21 days) from 5 farms (Quilez et al, 2008). Conversely, in Belgium, the cervid genotype was the predominant (n = 10) genotype identified in 18 positive lambs (aged 1 day -10 weeks), from 10 farms (Geurden et al, 2008). In Maryland, USA, the cervid genotype and C. bovis were the predominant species identified in both ewes and lambs (Santin et al, 2007), and were also the predominant species infecting post-weaned sheep in Western Australia (Ryan et al 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Three genotypes of G. duodenalis have been identified in sheep, genotype E, and the two zoonotic genotypes, genotype A and B (Giangaspero et al, 2005, Ryan et al, 2005, Aloisio et al, 2006, van der Giessen et al, 2006Castro-Hermida et al, 2007;Santin et al, 2007;Geurden et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

Yang

Jacobson

Gordon

et al. 2009

Veterinary Parasitology

100

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t 2 AbstractA total of 477 fecal samples from pre-weaned sheep from 5 different farms in the south west of Western Australia were screened for the presence of Cryptosporidium and Giardia using PCR. There were substantial differences in prevalence between the farms and overall prevalence was 24.5% and 11.1% respectively for Cryptosporidium and Giardia. At the 18S locus, 66 Cryptosporidium positives were identified, the majority of which were C. bovis (n = 52), followed by the cervid genotype (n= 10) and C. parvum (n = 2). At a second diagnostic locus, using C. parvum and C. hominis specific qPCR primers, 63 C. parvum positives were identified, some of which were co-infections with C. bovis. The C. parvum/C. hominis qPCR was more sensitive than the nested 18S PCR at detecting C. parvum. This may be due to the low numbers of oocysts present, as quantitation data indicated that all the C. parvum detected was present in low numbers (1-10 oocysts). It may also be that using C. parvum-specific primers is necessary to determine the true prevalence of C. parvum. Amongst Giardia positive isolates, G. duodenalis genotype E (livestock) was the most prevalent (36/53), with G.duodenalis genotype A detected in 5 positive isolates. There were also 11 mixed A and E infections detected. The findings of the present study indicate that pre-weaned lambs are not an important source of zoonotic Giardia genotypes in Australia but may be an important source of zoonotic Cryptosporidium.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

Yang

Jacobson

Gordon

et al. 2009

Veterinary Parasitology

100

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“…Because sheep can harbour C. parvum, they should be considered a potential source of infection of Cryptosporidium either by direct transmission or by contamination of the environment. As with cattle, there appears to be both geographical and age-related differences in the prevalence of zoonotic and non-zoonotic genotypes in sheep based on recent molecular characterization studies worldwide Geurden et al 2008;Quilez et al 2008;Mueller-Doblies et al 2008;Paoletti et al 2009;Diaz et al 2010;Robertson et al 2010;Wang et al 2010;Fiuza et al 2011b;Shen et al 2011). A recent longitudinal study of Cryptosporidium in meat lamb farms in southern Western Australia reported that Cryptosporidium prevalences at individual samplings ranged between 18·5 and 42·6% in lambs and were <10% in the ewes.…”

Section: Sheepmentioning

confidence: 99%

Cryptosporidiumspecies in Australian wildlife and domestic animals

Ryan

Power

2012

Parasitology

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SUMMARYCryptosporidiumis an important enteric parasite that is transmitted via the fecal-oral route, water and food. Humans, wildlife and domestic livestock all potentially contributeCryptosporidiumto surface waters. Most species ofCryptosporidiumare morphologically indistinguishable and can only be identified using molecular tools. Over 24 species have been identified and of these, 7Cryptosporidiumspecies/genotypes are responsible for most human cryptosporidiosis cases. In Australia, relatively few genotyping studies have been conducted. SixCryptosporidiumspecies (C. hominis,C. parvum,C. meleagridis,C. fayeri,C. andersoniandC. bovis)have been identified in humans in Australia. However, little is known about the contribution of animal hosts to human pathogenic strains ofCryptosporidiumin drinking water catchments. In this review, we focus on the available genotyping data for native, feral and domestic animals inhabiting drinking water catchments in Australia to provide an improved understanding of the public health implications and to identify key research gaps.

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“…and Giardia spp. are gastro-intestinal protozoa that affect a wide range of mammals (Geurden et al, 2008;Feng and Xiao, 2011), including sheep. The prevalence of Cryptosporidium and Giardia in sheep varies between studies conducted worldwide but for Cryptosporidium generally ranges from 15%-27% in lambs (Santín et al, 2007;Robertson et al, 2010;Ye et al, 2013;Yang et al, 2014a) and for Giardia generally ranges from 1.5-55.6% (Feng and Xiao, 2011).…”

Section: Introductionmentioning

confidence: 99%

Greater intensity and frequency of Cryptosporidium and Giardia oocyst shedding beyond the neonatal period is associated with reductions in growth, carcase weight and dressing efficiency in sheep

Jacobson

Williams

Yang

et al. 2016

Veterinary Parasitology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Haemonchus status (positive or negative) with lamb production were assessed using general linear models (HSCW and dressing %) and linear mixed effects models (live weight). HighCryptosporidium parvum shedding was associated with lower live weight, ranging 2.31-4.52kg over the 3 sampling occasions. Cryptosporidium parvum shedding was associated 3 with less HSCW in high (3.22kg less) and low (3.22kg less) shedding lambs post-weaning, and high (2.21kg less) and low (2.60kg less) shedding lambs pre-slaughter as well as lower dressing % (2.7% lower in high shedding lambs post-weaning). Cryptosporidium (all species) shedding pre-slaughter was associated with reduced dressing % in both high (1.25% lower) and low (1.21% lower) shedding lambs. Giardia shedding pre-slaughter was associated with 0.59kg less HSCW in high shedding lambs. Increased frequency of C.parvum and Giardia shedding in a specific animal (repeated detection) were associated with Abbreviations:HSCW: hot standard carcase weight WEC: Faecal worm egg count

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Prevalence and molecular characterisation of Cryptosporidium and Giardia in lambs and goat kids in Belgium

Cited by 148 publications

References 14 publications

Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

Prevalence and molecular characterisation of Cryptosporidium and Giardia species in pre-weaned sheep in Australia

Cryptosporidiumspecies in Australian wildlife and domestic animals

Greater intensity and frequency of Cryptosporidium and Giardia oocyst shedding beyond the neonatal period is associated with reductions in growth, carcase weight and dressing efficiency in sheep

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