Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran

Abstract

Background: Staphylococcus aureus is one of the major causes of community-and hospital-acquired infections, with methicillinresistant strains showing the highest rates of morbidity and mortality. In our previous experiment, isolates, which were also used in the present study, were assessed using multilocus sequence typing (MLST). The sequence types (STs) were determined and documented in the corresponding database. Objectives: In the current study, the isolates were subjected to genotyping with coagulase, SCCmec, and agr typing methods. Methods: A total of 54 isolates were evaluated by polymerase chain reaction (PCR) assay for mecA gene, Sccmec typing, and finally PCR-restriction fragment length polymorphism (RFLP) for coagulase (coa) gene using Alul enzyme. MLST of the isolates showed that the majority of methicillin-resistant S. aureus (MRSA) isolates belonged to ST239. Results: Phenotypic and genotypic tests revealed that 21% of the isolates were MRSA. PCR-RFLP test for coa gene showed similar patterns of MRSA isolates. The majority of the isolates were community-acquired and belonged to the Sccmec type IV, whereas the remaining were hospital-acquired and classified as type I (22.2%) and type III (2.2%). Conclusions: Most of the isolates belonged to agr type I, followed by type II and type III. Agar dilution method showed higher sensitivity and specificity, compared to the disk diffusion method. The majority of the isolates were community-acquired and belonged to Sccmec type IV and agr type I, whereas the remaining were hospital-acquired and classified as types I (22.2%), type III (2.2%), and agr type I.

Molecular analysis of methicillin-resistant Staphylococcus aureus isolates from four teaching hospitals in Iran: The emergence of novel MRSA clones

“…In a study conducted by Goudarzi et al [24] in the city of Tehran, ST22 has been documented as the third most commonly detected MRSA clone though the mentioned strains belonged to ST22-SCCmec IV in comparison to our MRSA ST22 which was ST22-SCCmec V. These ndings highlight the importance of using several typing methods simultaneously to achieve better analysis. In previous studies of MRSA and MSSA strains in the cities of Isfahan and Tehran the majority of MRSA isolates belonged to ST239 [25].…”

“…In the present study, in contrast SCCmec type V was the most prevalent SCCmec type identi ed among our clinical isolates of MRSA. However recent studies conducted in Armenia and Iran, SCCmec types V and IV were the most types that identi ed among MRSA isolates from hospitals respectively [13,25] Also in a study by Hallin et al [26] in Belgium, most clinical MRSA strains belonged to SCCmec types IV. These results could con rm the rotation of clones between community and hospital.…”

Molecular analysis of methicillin-resistant Staphylococcus aureus isolates from four teaching hospitals in Iran: The emergence of Novel MRSA ST- SCCmec clones

“…In the present study, in contrast SCCmec type V was the most prevalent SCCmec type identified among our clinical isolates of MRSA. However recent studies conducted in Armenia and Iran, SCCmec types V and IV were the most types that identified among MRSA isolates from hospitals respectively [8,25] Also in a study by Hallin et al [26] in Belgium, most clinical MRSA strains were belonged to SCCmec types IV. These results could confirm the rotation of clones between community and hospital.…”

“…These findings highlight the importance of using several typing methods simultaneously to achieve better analysis. In previous studies of MRSA and MSSA strains in the cities of Isfahan and Tehran the majority of MRSA isolates belonged to ST239 [25]. The high prevalence of ST239 has been documented from many Asian countries especially India, Pakistan, Vietnam, Thailand, Taiwan, China, Sri Lanka, and Singapore [27].…”

Frequency of methicillin-resistant Staphylococcus aureus (MRSA) in nose and cellular phone of medical and non-medical personnel of emergency departments of Ghaem hospital in Mashhad city

“…MRSA is one of the most prevalent pathogens in the world that is responsible for a wide range of nosocomial infections. 5,6 Studies have shown that contaminated medical staff (including doctors, nurses, and even service personnel in departments) are common sources of antibiotic-resistant Staphylococcus aureus epidemics in the hospital. MRSA which are separated from hospitals are called Healthcare-associated MRSA (HA-MRSA), and those which are separated from the community are named Community-Acquired MRSA (CA-MRSA).…”