Prevalence and genotypic (G and P) determination of porcine group A rotaviruses from different regions of India

Abstract

Group A rotaviruses can infect both humans and animals and have been recognized as an important cause of diarrhea in porcine. In this study, we report the prevalence and molecular epidemiology of rotaviruses detected in piglets in different regions of India. A total 275 fecal samples (180 diarrheal and 95 non-diarrheal) from piglets were collected from the western (135), southern (60), northern (20), and North-Eastern Hill (NEH) (60) regions of India and tested for rotaviruses. All the samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reverse transcription-polymerase chain reaction (RT-PCR). Rotaviruses were detected in 10.18 % of samples by SDS-PAGE and/or RT-PCR with a maximum of 30 % from the NEH region followed by 7.4 % from the western region. Samples from the southern and northern regions were found to be negative. Only 10 isolates were subjected to genotypic characterization using amplification of VP7 and VP4 genes followed by two separate multiplex PCR assays for G genotyping and another two for P genotyping using genotype-specific primers. Of these, three isolates could be typed as G4 specificity, one with G9, and three as P[6] leading to identification of an uncommon strain, G4P[6]. One isolate was further confirmed by nucleotide sequencing. The data demonstrate genetic diversity of porcine rotavirus strains and suggest that pig farms may serve as potential reservoirs for human infections.

Phylogenetic Analyses of the VP4 and VP7 Genes of Porcine Group A Rotaviruses in São Paulo State, Brazil: First Identification of G5P[23] in Piglets

“…The G9 genotype has been classified into an unusual category that includes human types (18). In addition, the interspecies transmission of group A rotaviruses between humans and pigs may occur frequently, as noted in other reports (7,16,(19)(20)(21)(22)(23)(24)(25)(26).…”

“…The P [6] type has been identified in piglets (16,19,27). This genotype, detected in the present study, demonstrated the highest level of identity with a human strain (89.8% to the sequence with accession number L33895), which is in agreement with other reports.…”

Salmonella daarle and Salmonella hiduddify associated with acute gastroenteritis in piglets in India

“…Dubal et al (2013) reported the presence of Rotavirus in piglet diarrhoea from the North Eastern Hilly Region of India from 10.18% by SDS-PAGE to 30.00% by RT-PCR, which is indicative of the role of Rotavirus in piglet diarrhoea in this region. In another study from our laboratory, Picobirnavirus was detected in 4.59% diarrheic and 5.60% healthy pigs from NER India (unpublished data).…”

Comparison of agarose gel electrophoresis and RNA-PAGE for rapid detection of rotavirus from faecal samples

“…RNA-PAGE and AGE. In majority of the earlier studies, long pattern seems to be dominant (Brown et al 1988;Broor et al 1993;Dubal et al 2013). The RNA patterns of the 38 animal strains were analysed (Pongsuwanne et al 1989) using PAGE and showed long RNA profiles, except for two porcine and two bovine specimens.…”