Prevalence and Genotype Characterization of Blastocystis hominis Among the Baghmalek People in Southwestern Iran in 2013 - 2014

Khoshnood, Saleh; Rafiei, Abdollah; Saki, Jasem; Alizadeh, Kobra

doi:10.5812/jjm.23930

Cited by 37 publications

(28 citation statements)

References 36 publications

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“…15,28,37 On the contrary, no difference in the infection rates was documented between the genders by other studies. 19,30,38 Previous publications reported high prevalence rates among individuals aged 5-59, 30 in adults aged ≥ 18 years, 15 and those aged 18-30 years 31 . Li and others demonstrated that individuals aged 60 years and above had the highest rate of Blastocystis spp.…”

Section: Discussionmentioning

confidence: 99%

“…9 However, other studies found no significant relation between the infection prevalence and age. 38,39 These contradictory results can be explained by the local determinants such as the environmental conditions that affect the fecal-oral route of transmission among individuals of different ages and genders. 15 On the other hand, number of the participants aged 7-19 years was low (3.9%), which might also have affected the results of statistical analyses in the current study.…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology and Prevalence of Blastocystis spp. in North Cyprus

Seyer¹,

Karasartova²,

Ruh³

et al. 2017

Am J Trop Med Hyg

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Abstract. This study was conducted to investigate the prevalence of Blastocystis spp. and its subtypes (STs) in North Cyprus; and to evaluate the presence of this parasite and its STs with respect to demographic, socioeconomic, and epidemiological factors, as well as gastrointestinal symptoms. Stool samples were collected from 230 volunteers. Each participant also filled out a questionnaire. The samples were examined microscopically by native-Lugol and trichrome methods and further tested by polymerase chain reaction (PCR) and sequencing. Prevalence of Blastocystis spp. infection was found to be 10.5%, 10.5%, and 27.8%, by direct microscopy, trichrome method, and PCR, respectively. No other parasites were detected in the specimens except Giardia spp. (n = 2; 0.8%) and Entamoeba coli (n = 1; 0.4%). The most common Blastocystis STs were ST3 (20; 31.2%), ST2 (18; 28.2%), ST1 (8; 12.5%), and ST4 (7; 11%); whereas other STs were identified as ST6 (3; 4.7%), ST7 (2; 3.2%), and non-ST (6; 9.4%). Presence of Blastocystis spp. and its STs was not significantly related to any of the demographic, socioeconomic, and epidemiological factors. Furthermore, no significant association of Blastocystis spp. and its STs with gastrointestinal symptoms was found. This study is the first investigation of the epidemiology of Blastocystis spp. in North Cyprus. Distribution of Blastocystis spp. and its STs among demographic, socioeconomic, and epidemiological factors showed complete homogeneity. Presence of the parasite and its STs was not significantly related with the gastrointestinal symptoms among symptomatic and asymptomatic individuals. These findings suggest that Blastocystis spp. may be part of the intestinal flora in humans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epidemiology and Prevalence of Blastocystis spp. in North Cyprus

Seyer¹,

Karasartova²,

Ruh³

et al. 2017

Am J Trop Med Hyg

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show abstract

“…The forward (Blast 505-532: 5′-GGAGGTAGTGACAATAAATC-3′) and reverse (Blast 998-1017: 5′-TGCTTTCGCACTTGTTCATC -3′) primers were used for amplification of the SSU-rRNA gene. 22 The PCR reaction was performed in a final volume of 25 μl. Each reaction contained 12.5 μl of the PCR master mix (2x Master Mix RED (Ampliqon, Denmark), (1.25 U Taq DNA polymerase, 200 μM of dNTPs and 1.5 mM of MgCl2), 0.5 μl of each primer (12.5 pmol), 5 μl of template DNA and 6.5 μl of double-distilled water.…”

Section: Dna Extraction and Pcrmentioning

confidence: 99%

“…16 The molecular studies conducted for the genotype identification of Blastocystis isolated from humans in Iran have reported the ST1, ST2, ST3, ST4, ST5, ST6 and ST7 genotypes. [19][20][21][22][23][24][25][26][27] The result of many studies https://doi.org/10.1016/j.cegh.2020.02.010 Received 11 September 2019; Received in revised form 2 January 2020; Accepted 14 February 2020 that were performed in Iran have showed that subtype 3 is the most common subtype of Blastocystis. 19,21,[23][24][25]27 Considering the fact that different subtypes of Blastocystis may be associated with geographical distribution, hosts and pathogenicity of parasite, 19,28 therefore, identification of the subtypes of Blastocystis is necessary for epidemiological studies.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and subtype identification of Blastocystis isolated from human in Shiraz city, southern Iran

Haghighi

Talebnia

Mikaeili

et al. 2020

Clinical Epidemiology and Global Health

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“…In Iran, information about Blastocystis subtypes is restricted to a few studies (14)(15)(16)(17) and only one study has investigated the relationship between Blastocystis sp. and IBS (18).…”

Section: Introductionmentioning

confidence: 99%

Comparative Prevalence of Blastocystis in Patients with the Irritable Bowel Syndrome and Healthy Individuals: A Case Control Study

Beiromvand

Hashemi

Arjmand

et al. 2017

Jundishapur J Microbiol

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Background: Blastocystis is one of the most common anaerobic protozoa found in the intestinal tract of humans and various animals, with a worldwide distribution. The parasite has been linked to the pathogenesis of the irritable bowel syndrome (IBS), previously. Objectives: The aim of this study was to evaluate the prevalence of Blastocystis in IBS patients compared to healthy individuals. Methods: The collected feces from 152 patients with Gastrointestinal (GI) symptoms, and 130 healthy volunteers from Ahvaz, southwest Iran, were examined using the direct saline smear, Lugol's iodine staining, and inoculated in a Jones' medium for Blastocystis detection. The DNA was extracted from all culture-positive samples, and then the polymerase chain reaction (PCR) was performed by the SSU-rDNA gene. Results: Blastocystis was identified in 18 (6.4%) samples, including two (1.3%) of the IBS patients and 16 (12.3%) of the control group by microscopy. Stool culture was positive in 15 with IBS, one without IBS, and 40 control samples. From these, the expected 600 bp fragments of the SSU-rDNA gene were identified in 15 (27.3%) cases and 40 (72.7%) controls. Subtypes (STs) 1, 2, and 3 were identified from the 54 successfully sequenced samples. Subtype 3 was the most common ST with the frequency of 46.3%, followed by ST2, 37% and ST1, 16.7% in the case and control groups. The highest frequency of Blastocystis STs (27.8%) was identified in the age group of 31-40 years and the lowest was found in the age groups of under 10 years and over 81 years. Conclusions:The findings of the current study showed that Blastocystis was more common in the control group compared to the IBS patients. Therefore, our findings highlight the contrast between Blastocystis infection and GI disorders. Furthermore, these results support the hypothesis that Blastocystis could be a GI health marker.

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Prevalence and Genotype Characterization of Blastocystis hominis Among the Baghmalek People in Southwestern Iran in 2013 - 2014

Cited by 37 publications

References 36 publications

Epidemiology and Prevalence of Blastocystis spp. in North Cyprus

Epidemiology and Prevalence of Blastocystis spp. in North Cyprus

Prevalence and subtype identification of Blastocystis isolated from human in Shiraz city, southern Iran

Comparative Prevalence of Blastocystis in Patients with the Irritable Bowel Syndrome and Healthy Individuals: A Case Control Study

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