Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia

Munkhjargal, Tserendorj; Sivakumar, Thillaiampalam; Battsetseg, Badgar; Nyamjargal, Tserendorj; AbouLaila, Mahmoud; Purevtseren, Byambaa; Bayarsaikhan, Dorj; Byambaa, Badarch; Terkawi, Mohamad Alaa; Yokoyama, Naoki; Igarashi, Ikuo

doi:10.1016/j.meegid.2013.02.005

Cited by 67 publications

(43 citation statements)

References 27 publications

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“…The 18S rRNA gene is widely used for phylogenetic analysis of equine piroplasms (BHOORA et al, 2009;HALL et al, 2013;MUNKHJARGAL et al, 2013). Herein, the genetic composition and the phylogenetic positioning of T. equi and B. caballi sequences amplified among equids from São Luís, Maranhão state, Brazil was assessed.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Braga

Costa

Gomes

et al. 2017

Rev. Bras. Parasitol. Vet.

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Equine piroplasmosisis, a tick-borne disease caused by the intra-erythrocytic protozoans Babesia caballi and Theileria equi, has economic importance due to the international trade and the increased movement of horses all over the world. The goal of this study was to evaluate the occurrence of phylogenetic diversity of T. equi and B. caballi genotypes among infected equids from São Luís Island, state of Maranhão, northeastern Brazil. Between December of 2011 and June of 2012, EDTA-blood and serum samples were collected from 139 equids (90 donkeys, 39 horses and 10 mules). From 139 serum samples submitted to ELISA assay, IgG antibodies to T. equi and B. caballi were detected in 19.4% (27/139) and 25.2% (35/139), respectively. Among sampled animals, 21.6% (30/139) and 55.4% (77/139) were positive for cPCR assays for T. equi and B. caballi, based on ema-1 and rap-1 genes, respectively. Overall, the T. equi sequences (n=7) submitted to Maximum Likelihood analysis (based on a 18S rRNA fragment of 1700 bp after alignment) grouped into three main groups, which were subdivided in eight clusters. The present work showed that different genotypes of T. equi and B. caballi circulate among equids in Brazil.Keywords: Babesia caballi, Theileria equi, phylogeny, 18S rRNA. ResumoA piroplasmose equina, uma doença transmitida por carrapatos e causada pelos protozoários intra-eritrocíticos Babesia caballi e Theileria equi, tem importância econômica devido ao comércio internacional e ao aumento do movimento de cavalos em todo o mundo. O objetivo do presente estudo foi mostrar a diversidade filogenética de T. equi e B. caballi infectando cavalos, burros e jumentos na Ilha de São Luís, Estado do Maranhão, Nordeste do Brasil. Entre dezembro de 2011 e junho de 2012, amostras de sangue com EDTA e soro de foram coletadas de 139 equídeos (90 jumentos, 39 cavalos e 10 burros). Dentre as 139 amostras de soro submetidas ao ensaio de ELISA, foram detectados anticorpos IgG contra T. equi e B. caballi em 19,4% (27/139) e 25,2% (35/139), respectivamente. Entre os animais amostrados, 21,6% (30/139) e 55,4% (77/139) foram positivos por meio dos ensaios de cPCR para T. equi e B. caballi, com base nos genes ema-1 e rap-1, respectivamente. No geral, as sequências T. equi (n = 7) submetidas à análise de Máxima Verossimilhança (baseada em um fragmento do 18S rRNA de 1700 pb, após o alinhamento) foram agrupadas em três grupos principais, os quais foram subdivididos em oito grupos. O presente trabalho mostrou que diferentes genótipos de T. equi e B. caballi circulam entre equídeos no Brasil.Palavras-chave: Babesia caballi, Theileria equi, filogenia, 18S rRNA.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Braga

Costa

Gomes

et al. 2017

Rev. Bras. Parasitol. Vet.

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“…Advanced management and disease control programs reduce the chance of infection in equids kept for recreational purposes by both molecular and serological methods (22.67 and 10.88 %, respectively), while open grazing practices in equids used for commercial purposes enhance the chance of T. equi infection (Moretti et al 2010). In international trading, cELISA is considered as official test because it shows both current and past exposures of the parasite; on other hand, molecular test targets only the DNA of parasite, so for epidemiological studies, combination of both should be used (Munkhjargal et al 2013) because PCR shows the presence of parasite and cELISA explores the history of animal regarding the disease. Moreover, the current study has depicted the possible absence of B. caballi in both conducive and non-conducive areas of Punjab.…”

Section: Discussionmentioning

confidence: 99%

“…Diagnosis by PCR has been found sensitive enough to detect parasite DNA from 2.5-μl blood sample with a parasitaemia of 0.000001 %. Thus, for cross-sectional study on epidemiological and carrier status of EP, a combination of serological and molecular diagnostic method tests (Machado et al 2012;Munkhjargal et al 2013) was adopted in the present study. The scarcity of enough literature on the epidemiology of these two causative agents of EP in Punjab state province (India) has prompted authors to undertake this pioneer investigation.…”

Section: Introductionmentioning

confidence: 99%

Theileria equi and Babesia caballi infection of equids in Punjab, India: a serological and molecular survey

Sumbria

Singla

Sharma

2015

Trop Anim Health Prod

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A cross-sectional study was conducted in Submountain undulating, Undulating plain, Western and Western plain agro-climatic zones of Punjab province, India, to determine the prevalence, agreement between diagnostic tests and associated related risk factors of Theileria equi and Babesia caballi infection in equids (horses, donkey, mules). An overall prevalence of 14.14 and 0.0% of T. equi and B. caballi was recorded by multiplex polymerase chain reaction targeting 18S ribosomal RNA (rRNA) for both the parasites and 75 and 1.11% by competitive enzyme-linked immunosorbent assay in a representative sample of 180 animals. Only two animals with positive antibody titre from B. caballi and none with PCR indicated T. equi as the predominant haemoprotozoan responsible for equine piroplasmosis in the study area. Among the PCR-positive animals, presence of tick vectors in farm vicinity was the most influential associated with T. equi infection (P = 0.002; odds ratio (OR) 9.30; 95% confidence interval (CI) = 3.32-27.10). For animals with higher anti-T. equi antibody titres, strong association of sero-prevalence for T. equi was recorded with age, sex, usage, tick infestation and deworming/vaccination status of host animals and farm management strategies. The study has demonstrated the possible absence of B. caballi in both conducive and non-conducive areas of Punjab and demonstrated T. equi as the potential agent of equine piroplasmosis in Punjab.

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“…During the last decade, several molecular studies illustrated lower host-specificity of piroplasmids (Fritz, 2010;Hamel et al, 2012;Qablan et al, 2012) and suggested possible transmissions between equines and dogs. So far, five T. equi and three B. caballi genotypes have been described, with more diversity reported within T. equi (Munkhjargal et al, 2013). The aims of this study were (i) to determine the prevalence and diversity of piroplasmids infecting feral and domestic equines in the Danube Delta area, (ii) to assess the intraspecific genetic diversity of detected piroplasmids, (iii) to assess the extent of cross transmissions between equines and domestic dogs.…”

Section: Introductionmentioning

confidence: 99%

Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir

Gallusová

Qablan

D’Amico

et al. 2014

Veterinary Parasitology

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Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia

Cited by 67 publications

References 27 publications

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Genetic diversity of piroplasmids species in equids from island of São Luís, northeastern Brazil

Theileria equi and Babesia caballi infection of equids in Punjab, India: a serological and molecular survey

Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir

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