Prevalence and Genetic Characteristics of Methicillin-Resistant<i>Staphylococcus aureus</i>and Coagulase-Negative Staphylococci Isolated from Oral Cavity of Healthy Children in Japan

Hirose, Mina; Aung, Meiji Soe; Fukuda, Atsushi; Murata, Yukie; Saitoh, Masato; Kobayashi, Nobumichi

doi:10.1089/mdr.2018.0333

Cited by 13 publications

(15 citation statements)

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“…This study demonstrated a relatively high prevalence of diverse staphylococcal species in the oral cavity. Similar to previous studies, we identified S. aureus as the most frequent oral isolate [ 17 , 23 ]. Aside from S. aureus , we isolated also twelve different species of CoNS.…”

Section: Discussionsupporting

confidence: 88%

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Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Garbacz

Wierzbowska

Kwapisz

et al. 2021

Journal of Oral Microbiology

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Background: The use of antibiotics in dentistry is associated with the emergence and spread of antibiotic-resistant microorganisms, including commensal staphylococci. Methods: A total of 367 oral samples were collected, from which staphylococci were isolated and identified by using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF). The antibiotic susceptibility of the isolates was determined and molecular characteristics for methicillin-resistant staphylococci was performed. Results: A total of 103 coagulase-negative staphylococci (CoNS), among them S. warneri, S. haemolyticus, S. saprophyticus, S. pasteuri, S. epidermidis, S. hominis, S. xylosus, S. equorum, S. kloosii, S. succinus, S. cohnii , and S. simulans , were confirmed by MALDI-TOF. Resistance to most tested antibiotics was statistically higher in CoNS than in S. aureus isolates ( P -value < 0.05). CoNS isolates showed high resistance to penicillin ( S. saprophyticus 88.9%), erythromycin ( S. haemolyticus 84.6%), fusidic acid ( S. saprophyticus 77.8%), co-trimoxazole ( S. epidermidis 71.4%), gentamicin ( S. warneri 63.8%), and tetracycline ( S. saprophyticus 55.6%). Multidrug resistance was largely observed, especially among S. haemolyticus and S. saprophyticus species. Methicillin-resistance in S. haemolyticus (38.5%), S. saprophyticus (22.2%) and S. aureus (13.5%) was associated with the presence of the mec A gene and SCC mec type IV or V. Conclusion: Coagulase-negative staphylococci, especially S. haemolyticus and S. saprophyticus , seem to be a reservoir of methicillin resistance and multidrug resistance in the oral cavity.

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Section: Discussionsupporting

confidence: 88%

“…Aside from S. aureus , we isolated also twelve different species of CoNS. The presence of various CoNS species in the oral cavity was also demonstrated in recent studies conducted in Poland, Japan, and Argentina [ 23–25 ]. In a study of healthy persons and patients subjected to kidney transplantation, Majchrzak et al .…”

Section: Discussionmentioning

confidence: 55%

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Garbacz

Wierzbowska

Kwapisz

et al. 2021

Journal of Oral Microbiology

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“…ST45 and ST59 were two major MRSA clones in China (24); however, in this study, a proportion of these two clones was MSSA. ST45-MSSA was reported in our previous publication and other publication (20,41), whereas ST59-MSSA was seldomly reported in other studies. In contrast, ST30 was a major MRSA clone in nine Latin American countries (21) but all were MSSA in this study.…”

Section: Discussionmentioning

confidence: 65%

Active Surveillance, Drug Resistance, and Genotypic Profiling of Staphylococcus aureus Among School-Age Children in China

Liang

Gao

et al. 2021

Front. Med.

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Methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization predisposes individuals for endogenous infections and is a major threat to children. Recently, oxacillin/cefoxitin-susceptible mecA-positive S. aureus (OS-MRSA) has been reported worldwide. Herein, a prospective, cross-sectional study was conducted across five schools, representing three educational stages, in Guangzhou, China. Nasal swabs from 2,375 students were cultured for S. aureus and all isolates were subjected to antibiotic susceptibility testing phenotypically and confirmed by femB and mecA genetic detection; all the isolates were classified as MSSA, MRSA, or OS-MRSA. All strains were also analyzed by multi-locus sequence typing. Among the 2,375 swabs, S. aureus was detected in 744 children (31.3%, 95% CI: 25.9–36.7%), of whom 72 had MRSA (3.0%, 95% CI: 0.6–5.4%) and 4 had OS-MRSA (0.2%, 95% CI: 0.1–0.3%), of which an oxacillin- and cefoxitin-susceptible MRSA strain was identified. The prevalence of S. aureus and MRSA was higher in younger children. The highest percentage of drug resistance of the S. aureus isolates (n = 744) was to penicillin (85.5%), followed by erythromycin (43.3%) and clidamycin (41.0%). The most prevalent sequence types (STs) were ST30, ST45, and ST188 in MSSA, accounting for 38.7% of the total isolates, whereas ST45, ST59, and ST338 accounted for 74.6% of the MRSA isolates and ST338 accounted for 50.0% of the OS-MRSA isolates. The MRSA and OS-MRSA isolates (n = 76) were grouped into three clades and one singleton, with clonal complex (CC) 45 as the most predominant linkage. The top nine multi-locus sequence typing-based CCs (CC30, CC45, CC5, CC1, CC15, CC944, CC398, CC59, CC7) represented 86.7% of all S. aureus isolates. All CC30 isolates were resistant to erythromycin and clidamycin, and almost all these isolates were also resistant to penicillin (99.2%). The CC45 and CC59 isolates exhibited high resistance rates to oxacillin at 31.5 and 59.0%, respectively. This study provides updated data valuable for designing effective control strategies to mitigate the burden of disease and to improve the adequacy of empirical antimicrobial treatments for potentially harmful infections.

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“…In our previous study, the prevalence of MRSA and MR-CoNS in the oral cavity of healthy children and their genetic traits were analyzed in Hokkaido, the northern main island of Japan [ 29 ]. As a result, 6.3 and 50% of colonizing S. aureus and S. epidermidis were identified as being MR, respectively, with MRSA belonging to five STs (ST1, ST5, ST8, ST89 and ST120).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan

et al. 2021

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The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of Staphylococcus isolates from the oral cavity and skin (hand) of systemically healthy subjects with dental disease and dental staff in northern Japan. Among a total of 133 subjects (91 patients and 42 staff), 87 coagulase-positive Staphylococcus (83 S. aureus/4 S. argenteus) and 162 coagulase-negative Staphylococcus (CoNS) isolates were recovered from 59 (44.4%) and 95 (71.4%) subjects, respectively. Three oral isolates were methicillin-resistant S. aureus (MRSA) (3.6%, 3/83) that were genotyped as ST8-SCCmec-IVl, ST4775(CC1)-SCCmec-IVa and ST6562(CC8)-SCCmec-IVa. Remarkably, the ST6562 isolate harbored PVL genes on ΦSa2usa and type I ACME (arginine catabolic mobile element). Four methicillin-susceptible isolates were identified as S. argenteus belonging to ST1223 and ST2250, which harbored enterotoxin genes egc-2 and sey, respectively. Among the fourteen CoNS species identified, methicillin-resistant (MR) isolates were detected in five species (11 isolates, 13.3% of CoNS), with S. saprophyticus and S. haemolyticus being the most common. ACME was prevalent in only S. epidermidis and S. capitis. These findings indicated the potential distribution of USA300 clone-like MRSA, toxigenic S. argenteus and MR-CoNS in the oral cavity of dental patients.

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Prevalence and Genetic Characteristics of Methicillin-ResistantStaphylococcus aureusand Coagulase-Negative Staphylococci Isolated from Oral Cavity of Healthy Children in Japan

Cited by 13 publications

References 53 publications

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity

Active Surveillance, Drug Resistance, and Genotypic Profiling of Staphylococcus aureus Among School-Age Children in China

Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan

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