Prevalence and genetic basis of first-line drug resistance of<i>Mycobacterium tuberculosis</i>in Ca Mau, Vietnam

Callum, Jack; Nguyen, Phuong Chi; Martínez, Elena; Nguyễn, Vân Hà; Garden, Frances L.; Nguyen, Nhung Viet; Nguyen, Thu-Anh; Nguyen, Hoa B.; Nguyen, Son; Luu, Khanh Boi; Ho, Jennifer; Linh, Nguyễn Nhật; Britton, Warwick J.; Sintchenko, Vitali; Fox, Greg J.; Marks, Guy B.

doi:10.1183/23120541.00122-2022

Cited by 3 publications

(4 citation statements)

References 38 publications

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“…Distribution of the samples according to the demographic characteristics in tabular form (3). Sample percentages increased most rapidly between the ages of 31 and 40, with those between the ages of 21 and 30 following closely behind at 42%.…”

Section: Resultsmentioning

confidence: 99%

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Frequency of Mycobacterium tuberculosis in Pneumonia Patients and Prevalence of Virulence Genes

2023

JPTCP

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Tuberculosis is among the top 10 leading causes of death worldwide (TB). Tuberculosis is caused by the bacteria Mycobacterium tuberculosis. Acute pulmonary TB manifests as acute tuberculous pneumonia (TP). The development of drug-resistant TB, however, continues to pose a problem for TB treatment and efficient disease management. Different mutations in M. tuberculosis can lead to isolates that are rifampicin-resistant. The genes katG, inhA, and inhA's promoter are all involved in cell wall production, and most INH-R strains have mutations in these genes. The purpose of this research was to determine how often M. tuberculosis infection in patients suffering from pneumonia and also find the prevalence of Rifampicin and Isoniazid resistance isolates, and also to correlate the infection with the lipid profile of patients. This study included 200 subjects who are suffering from severe pneumonia. DNA have been extracted from all samples and then M. tuberculosis detection have been done by qRT-PCR. Lipid profile have also been estimated for all the included samples. Then detection of the antibiotic resistance have also done by qRT-PCR. The distribution of samples showed a significant difference among the different infection periods (chi-square= 44.19, P-Value= <0.001). The results showed that (7%) of the positive samples are resistance to Rifampicin. while resistance to Isoniazid are shown in 6.5% of the samples. The results of lipid profile showed a significant higher level in positive samples than negative samples regarding the serum level of total cholesterol (196.90 VS. 172.86, respectively), triglyceride (195.61 VS 152.33, respectively), and low density lipid (121.03 VS. 98.14, respectively).

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Section: Resultsmentioning

confidence: 99%

“…Resistance to rifampicin was observed in 3.5% (1.1 to 5.7%) and resistance to isoniazid in 19.8% (95% CI 14.7 to 24.9%). (Callum et al 2022). There is a comparable proportion of isoniazid resistance (16.6%) and rifampicin resistance (2.0%) in southern Vietnam, according to earlier studies (Huong et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Frequency of Mycobacterium tuberculosis in Pneumonia Patients and Prevalence of Virulence Genes

2023

JPTCP

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“…Multidrug-resistant TB (MDR-TB) results from M. tuberculosis strains that resist treatment with isoniazid and rifampicin, two primary anti-TB medications [ 3 ]. TB infection is treated with first-line drugs isoniazid and rifampicin.…”

Section: Introductionmentioning

confidence: 99%

“…Failure to comply with treatment or take adequate medication contributes to the development and spread of drug resistance in M. tuberculosis against all relevant antibiotics [ 5 ]. Therefore, MDR-TB is a TB caused by M. tuberculosis that is resistant to treatment with at least two of the first-line anti-TB drugs, including isoniazid and rifampicin [ 3 ]. Furthermore, extensively drug-resistant TB (XDR-TB) is defined as MDR-TB plus resistance to one of the fluoroquinolones and to one of the second-line injectable anti-TB drugs: amikacin, kanamycin, or capreomycin [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of multidrug-resistant Mycobacterium tuberculosis in upper Southern Thailand

Karaipoom,

Saengsawang,

Bromnavej

et al. 2024

Vet World

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Background and Aim: Mycobacterium tuberculosis causes global concern with tuberculosis (TB). Multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) pose additional challenges, as they resist multiple first-line drugs. This study investigated the occurrence of TB, antibiotic resistance due to inhA and katG gene mutations, and multidrug resistance in M. tuberculosis during fiscal years 2020–2022. Materials and Methods: Samples were gathered from hospitals in seven provinces of upper Southern Thailand. The study investigated the correlation between inhA and katG gene mutations in M. tuberculosis and the development of antimicrobial resistance and isoniazid resistance. Results: A total of 19,186 samples were sent to the Office of Disease Prevention and Control Region 11st, Nakhon Si Thammarat, Thailand. The results showed that 51% of the samples were obtained from patients located in Nakhon Si Thammarat, followed by Surat Thani provinces. Regarding the spatial distribution of TB-infected cases, the incidence of TB was high in the province, which has a moderate to high population density. The highest average occurrence of TB in this study was found in Phuket province (9.75/100,000 risk person-year). The detected isoniazid resistance was 394, 255, and 179 cases in 2020, 2021, and 2022, respectively. A total of 99 isolates were MDR, whereas four isolates were XDR. The antimicrobial resistance associated with the inhA mutation was 192, 142, and 105 isolates, respectively, whereas the resistance associated with the katG mutation was 249, 182, and 120 cases in 2020, 2021, and 2022, respectively. Conclusion: These findings contribute to the understanding of the occurrence of antibiotic-resistant TB that could lead to use as data for preventing MDR-TB. Keywords: inhA and katG genes, isoniazid, multi-drug resistance, Mycobacterium tuberculosis, upper Southern of Thailand.

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Population-wide active case finding as a strategy to end TB

Nguyen,

Jing Teo,

Zhao

et al. 2024

The Lancet Regional Health - Western Pacific

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Prevalence and genetic basis of first-line drug resistance ofMycobacterium tuberculosisin Ca Mau, Vietnam

Cited by 3 publications

References 38 publications

Frequency of Mycobacterium tuberculosis in Pneumonia Patients and Prevalence of Virulence Genes

Frequency of Mycobacterium tuberculosis in Pneumonia Patients and Prevalence of Virulence Genes

Occurrence of multidrug-resistant Mycobacterium tuberculosis in upper Southern Thailand

Population-wide active case finding as a strategy to end TB

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