Prevalence and drug resistance characteristics of carbapenem-resistant Enterobacteriaceae in Hangzhou, China

Abstract

With the abuse of antimicrobial agents in developing countries, increasing number of carbapenem-resistant Enterobacteriaceae (CRE) attracted considerable public concern. A retrospective study was conducted based on 242 CRE strains from a tertiary hospital in Hangzhou, China to investigate prevalence and drug resistance characteristics of CRE in southeast China. Bacterial species were identified. Antimicrobial susceptibility was examined by broth microdilution method or epsilometer test. Resistant β-lactamase genes were identified by polymerase chain reaction and sequencing. Genotypes were investigated by phylogenetic analysis. Klebsiella pneumoniae and Escherichia coli were the most prevalent types of species, with occurrence in 71.9% and 21.9% of the strains, respectively. All strains exhibited high resistance (> 70%) against β-lactam antibiotics, ciprofloxacin, trimethoprim-sulfamethoxazole, and nitrofurantoin but exhibited low resistance against tigecycline (0.8%) and minocycline (8.3%). A total of 123 strains harbored more than two kinds of β-lactamase genes. bla, bla, bla, and bla were the predominant genotypes, with detection rates of 60.3%, 61.6%, 43.4%, and 16.5%, respectively, and were highly identical with reference sequences in different countries, indicating potential horizontal dissemination. IMP-4 was the most frequent class B metallo-lactamases in this study. In conclusion, continuous surveillance and effective prevention should be emphasized to reduce spread of CRE.

Molecular epidemiology and drug resistant mechanism in carbapenem-resistant Klebsiella pneumoniae isolated from pediatric patients in Shanghai, China

“…Moreover, the dissemination of bla IMP-4 -producing K . pneumoniae has been increasing[ 23 , 24 ]. NDM-1and IMP-4 were more frequent than KPC-2 and showed a multiclonal background.…”

Characterization of antimicrobial resistance genes inHaemophilus parasuisisolated from pigs in China

“…Cephalosporinases, which are naturally present in some enterobacterial species, can be mobilized by transposons and migrate via plasmids into other species. Moreover, the abuse of antimicrobial agents increases the number of carbapenem-resistant strains generating a public health concern ( Yang et al, 2017 ). In the Enterobacteriaceae, the bla TEM−1 β-lactamase is the predominant genotype ( Yang et al, 2017 ).…”

“…Moreover, the abuse of antimicrobial agents increases the number of carbapenem-resistant strains generating a public health concern ( Yang et al, 2017 ). In the Enterobacteriaceae, the bla TEM−1 β-lactamase is the predominant genotype ( Yang et al, 2017 ). In our study, we identified both bla TEM−1 and bla ROB−1 β-lactamase genes which are widespread among H. parasuis and Pasteurella spp ( Guo et al, 2012 ; San Millan et al, 2007 ).…”

Distribution of β-lactamase Gene and Genetic Context of blaKPC-2 in Clinical Carbapenemase-producing Klebsiella Pneumoniae Isolates

“…Isolates resistant to either or both carbapenems (imipenem and meropenem) tested were classi ed as CRKP [13] . The carbapenemase phenotype was further con rmed by the Carba NP test [14] . MDR, extensively drug-resistant (XDR) and pandrug-resistant (PDR) phenotypes were de ned according to a previous report [15] .…”