Prevalence and Diversity of Rotavirus Strains in Children with Acute Diarrhea from Rural Communities in the Limpopo Province, South Africa, from 1998 to 2000

Potgieter, Natasha; Beer, M.; Taylor, Maureen B.; Steele, A. Duncan

doi:10.1086/653561

Cited by 25 publications

(22 citation statements)

References 60 publications

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“…The present study shows a high prevalence and genotyping diversity of RVA in children ≤5 years of age living in Chókwè District, Southern Mozambique, and corroborates findings from a previous study conducted in the country showing the importance of RVA in acute diarrhea disease [Rappelli et al, ]. In our study, RVA infections were detected more frequently in children between 7 and 12 months of age, similar to reports from other areas of Southern Africa [Potgieter et al, ; Waggie et al, ].…”

Section: Discussionsupporting

confidence: 92%

“…In this study, RVA genotype G12P[8] was the prevalent genotype, corroborating previous reports conducted in Asian, European, American, and African continents, demonstrating the epidemiological relevance of this genotype worldwide [Cunliffe et al, ; Page et al, ; Potgieter et al, ; Jere et al, ; Page et al, ; Seheri et al, ; Bucardo et al, ; Luchs et al, ]. The high proportion of genotype G12 in African populations is believed to be associated with the emergence of genotype P[6] [Steele and Ivanoff, ; Page et al, ; Seheri et al, ].…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Langa

Thompson²,

Arnaldo³

et al. 2016

Journal of Medical Virology

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Acute diarrhea disease caused by Rotaviruses A (RVA) is still the leading cause of morbidity and mortality in children ≤5 years old in developing countries. An exploratory cross-sectional study was conducted between February and September, 2011 to determine the proportion of acute diarrhea caused by RVA. A total of 254 stool specimens were collected from children ≤5 years old with acute diarrhea, including outpatients (222 children) and inpatients (32 children), in three local health centers in Chókwè District, Gaza Province, South of Mozambique. RVA antigens were detected using enzyme immunoassay (EIA); the RVA G (VP7) and P (VP4) genotypes were determined by RT-PCR or analysis sequencing. Sixty (24%) out of 254 fecal specimens were positive for RVA by EIA; being 58 (97%) from children ≤2 years of age. RVA prevalence peaks in June and July (coldest and drier months) and the G[P] binary combination observed were G12P[8] (57%); G1P[8] (9%); G12P[6] (6%); and 2% for each of the following genotypes: G1P[6], G2P[6] G4P[6], and G9P[8]. Non-Typeable (NT) G and/or P genotypes were observed as follows: G12P [NT] (6%); G1P [NT], G3P[NT] and GNTP[NT] (4%). Considering the different GP combinations, G12 represented 67% of the genotypes. This is the first data showing the diversity of RVA genotypes in Mozambique highlighting the epidemiological importance of these viruses in acute diarrhea cases in children ≤2 years old. In addition, these findings will provide a baseline data before the introduction of the RVA monovalent (Rotarix(®) ) vaccine in the National Immunization Program in September 2015. J. Med. Virol. 88:1751-1758, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Langa

Thompson²,

Arnaldo³

et al. 2016

Journal of Medical Virology

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“…The G2P[6] strain was previously reported at a prevalence of 2.7% in children with acute diarrhoea in the same rural region in SA (Potgieter et al . ). In addition, the globally emergent G12 strain was detected in association with P[6] and P[8] VP4 types.…”

Section: Discussionmentioning

confidence: 97%

Applicability of Bio‐wipes for the collection of human faecal specimens for detection and characterisation of enteric viruses

Mans

Zyl

Taylor

et al. 2013

Tropical Med Int Health

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Abstractobjective To determine whether gastroenteritis viruses and other enteric viruses could be detected in faecal specimens collected with Bio-wipes.methods Faecal specimens, self-collected with Bio-wipes, from 190 individuals (94 diarrhoeal, 93 nondiarrhoeal, 3 unknown) were screened for eight human enteric viruses (enterovirus, hepatitis A virus, adenovirus, astrovirus, norovirus GI and GII, sapovirus and rotavirus) by real-time (reverse transcription)-polymerase chain reaction. Rotaviruses and noroviruses from positive specimens were genotyped.results At least one enteric virus could be detected in 82.6% (157/190) of faecal specimens. Mixed infections of up to four different viruses could be detected in both diarrhoeal and non-diarrhoeal specimens. Enteroviruses were detected most frequently (63.7%), followed by adenoviruses (48.4%) and noroviruses (32.2%). Genotyping was successful for 78.6% of rotaviruses and 44.8% of noroviruses.conclusions Bio-wipes provide a user friendly, easier method for stool collection that facilitates enteric virus detection and genetic characterisation.keywords Bio-wipe, enteric virus, faecal specimen, virus detection

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“…Both P[6] and P[11] are more commonly found in developing countries than developed ones, 61, 66, 67, 68, 69, 70, 71 particularly for P[6], which causes ~30% of RV cases in Africa. 50, 51, 52, 66, 67, 68 One possible reason could be the close contact between children and domestic animals in rural areas of developing countries, since these animals may be infected with P[6] and P[11] RVs due to shared HBGA ligands between humans and animals.…”

Section: Introductionmentioning

confidence: 99%

“…50, 51, 52, 66, 67, 68 One possible reason could be the close contact between children and domestic animals in rural areas of developing countries, since these animals may be infected with P[6] and P[11] RVs due to shared HBGA ligands between humans and animals. 36, 38, 64 Another possible reason for the high prevalence of P[6] in Africa could be the higher rates of Le a−b− (Lewis-negative) phenotype in African populations, 45, 46, 47 which possibly leads to a longer age-window with high expression levels of the HBGA precursors, since a correlation between P[6] RV infection and the Lewis-negative phenotype in children has been observed.…”

Section: Introductionmentioning

confidence: 99%

Histo-blood group antigens as receptors for rotavirus, new understanding on rotavirus epidemiology and vaccine strategy

Jiang

Liu

Tan

2017

Emerging Microbes & Infections

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The success of the two rotavirus (RV) vaccines (Rotarix and RotaTeq) in many countries endorses a live attenuated vaccine approach against RVs. However, the lower efficacies of both vaccines in many low- and middle-income countries indicate a need to improve the current RV vaccines. The recent discovery that RVs recognize histo-blood group antigens (HBGAs) as potential receptors has significantly advanced our understanding of RV diversity, evolution and epidemiology, providing important new insights into the performances of current RV vaccines in different populations and emphasizing a P-type-based vaccine approach. New understanding of RV diversity and evolution also raises a fundamental question about the ‘Jennerian' approach, which needs to be addressed for future development of live attenuated RV vaccines. Alternative approaches to develop safer and more cost-effective subunit vaccines against RVs are also discussed.

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Prevalence and Diversity of Rotavirus Strains in Children with Acute Diarrhea from Rural Communities in the Limpopo Province, South Africa, from 1998 to 2000

Abstract: The unusual serotype and genotype combinations of rotavirus circulating in the rural communities of the Limpopo Province highlight the need for more studies to monitor the geographic distribution of rotavirus strains in rural African settings.

Cited by 25 publications

References 60 publications

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Applicability of Bio‐wipes for the collection of human faecal specimens for detection and characterisation of enteric viruses

Histo-blood group antigens as receptors for rotavirus, new understanding on rotavirus epidemiology and vaccine strategy

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