Prevalence and diversity of integrons and associated resistance genes in faecal Escherichia coli isolates of healthy humans in Spain

Vinué, Laura; Sáenz, Yolanda; Somalo, Sergio; Escudero, Esther; Moreno, Miguel A.; Ruiz-Larrea, Fernanda; Torres, Carmén

doi:10.1093/jac/dkn331

Cited by 82 publications

(77 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The association of both classes of integrons with aadA genes has been demonstrated in numerous studies, for instance, as a part of the Tn7-like class 2 integron gene array dfrA1-sat1-aadA1 (30,51,52). The aadB marker, encoding resistance to gentamicin, kanamycin, and tobramycin, was identified in a single isolate as part of a 2.7-kb variable region within a class 1 integron along with aadA1 (1%) in a previously reported gene arrangement, aadB-aadA1 (30,52).…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Irish Cattle Farms

Karczmarczyk

Walsh

Slowey³

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

This study describes the genotypic characteristics of a collection of 100 multidrug-resistant (MDR) Escherichia coli strains recovered from cattle and the farm environment in Ireland in 2007. The most prevalent antimicrobial resistance identified was to streptomycin (100%), followed by tetracycline (99%), sulfonamides (98%), ampicillin (82%), and neomycin (62%). Resistance was mediated predominantly by strA-strB (92%), tetA (67%), sul2 (90%), bla TEM (79%), and aphA1 (63%) gene markers, respectively. Twenty-seven isolates harbored a class 1 integrase (intI1), while qacE⌬1 and sul1 markers were identified in 25 and 26 isolates, respectively. The variable regions of these integrons contained aminoglycoside, trimethoprim, and ␤-lactam resistance determinants (aadA12, aadB-aadA1, bla OXA-30 -aadA1, dfrA1-aadA1, dfrA7). Class 2 integrons were identified less frequently (4%) and contained the gene cassette array dfrA1-sat1-aadA1. Resistance to ampicillin, neomycin, streptomycin, sulfonamide, and tetracycline was associated with transferable high-molecular-weight plasmids, as demonstrated by conjugation assays. A panel of virulence markers was screened for by PCR, and genes identified included vt1, K5 in 2 isolates, papC in 10 isolates, and PAI IV 536 in 37 isolates. MDR commensal E. coli isolates from Irish cattle displayed considerable diversity with respect to the genes identified. Our findings highlight the importance of the commensal microflora of food-producing animals as a reservoir of transferable MDR.

show abstract

Section: Discussionmentioning

confidence: 95%

“…Their extensive dissemination could be attributed to their association with integrons and plasmids (13,31,30,51).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Irish Cattle Farms

Karczmarczyk

Walsh

Slowey³

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…dfrA17-aadA5 was the only gene cassette isolated from healthy farm workers in China (Li et al, 2010). Studies of commensal species isolated from healthy humans in Switzerland and Spain detected this same allele, although they reported the dfrA1-aadA1 arrangement to have a higher frequency (Cocchi et al, 2007;Vinué et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Globally dispersed mobile drug-resistance genes in Gram-negative bacterial isolates from patients with bloodstream infections in a US urban general hospital

Adams‐Sapper

Sergeevna-Selezneva

Tartof

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

Mobile drug-resistance genes with identical nucleic acid sequences carried by multidrug-resistant Escherichia coli strains that cause community-acquired infections are becomingly increasingly dispersed worldwide. Over a 2-year period, we analysed Gram-negative bacterial (GNB) pathogens from the blood of inpatients at an urban public hospital to determine what proportion of these isolates carried such globally dispersed drug-resistance genes. Of 376 GNB isolates, 167 (44 %) were Escherichia coli, 50 (13 %) were Klebsiella pneumoniae, 25 (7 %) were Pseudomonas aeruginosa, 25 (7 %) were Proteus mirabilis and 20 (5 %) were Enterobacter cloacae; the remainder (24 %) comprised 26 different GNB species. Among E. coli isolates, class 1 integrons were detected in 64 (38 %). The most common integron gene cassette configuration was dfrA17-aadA5, found in 30 (25 %) of 119 drug-resistant E. coli isolates and in one isolate of Moraxella morganii. Extended-spectrum b-lactamase (ESBL) genes were found in 16 E. coli isolates (10 %). These genes with identical sequences were found in nearly 40 % of bloodstream E. coli isolates in the study hospital, as well as in a variety of bacterial species from clinical and non-clinical sources worldwide. Thus, a substantial proportion of bloodstream infections among hospitalized patients were caused by E. coli strains carrying drug-resistance genes that are dispersed globally in a wide variety of bacterial species.

show abstract

“…In Spain, 3 out of 100 (3.0%) E.coli strains revealed the presence of class 2 integron (Vinue et al, 2008). Su et al reported 4 out of 111 (3.6%) E. coli strains harboring class 2 integron with a classic cassette array dfrA1-sat1-aadA1.…”

Section: Class 2 Integronmentioning

confidence: 99%

Role of integrons in antimicrobial resistance: A review

Gao¹,

Li²,

Liu³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Antibiotic resistance in bacteria still remains one of the leading concerns in global public health. Horizontal transfer of resistance genes is considered to be a major cause to facilitate the rapid spread of antibiotic resistance in microbes. In recent years, the role of integrons as mobile genetic elements playing a central role in horizontal transfer of antibiotic resistance have been well studied and documented. Nevertheless, up to date, most of the available investigations and studies on integron had focused on class 1 integrons of Gram-negative microorganisms. In the last decade, class 4 integrons (also known as super integrons) has been considered a major concern in antimicrobial resistance, as well as, a significant factor in bacterial genome evolution; however, most reports had been limited to Vibrio. Thus, this review is aimed at summarizing the occurrence and prevalence of integrons in bacterial isolates, with the focus on class 1 integron within Gram-positive bacteria, class 2 and 3 integrons.

show abstract

Prevalence and diversity of integrons and associated resistance genes in faecal Escherichia coli isolates of healthy humans in Spain

Abstract: A high prevalence of integrons was detected in faecal E. coli of healthy humans. Individuals in the community could be a reservoir of integron-containing E. coli isolates.

Cited by 82 publications

References 8 publications

Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Irish Cattle Farms

Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Irish Cattle Farms

Globally dispersed mobile drug-resistance genes in Gram-negative bacterial isolates from patients with bloodstream infections in a US urban general hospital

Role of integrons in antimicrobial resistance: A review

Contact Info

Product

Resources

About