Prevalence and diversity of extended-spectrum ß-lactamases in faecal Escherichia coli isolates from healthy humans in Spain

Vinué, Laura; Sáenz, Yolanda; Martínez, S.; Somalo, Sergio; Moreno, Miguel A.; Torres, Carmén; Zarazaga, Myriam

doi:10.1111/j.1469-0691.2009.02803.x

Cited by 79 publications

(59 citation statements)

References 21 publications

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“…bla CTX-M genes were often found in plasmids of different sizes (from 78 kb to 138 kb), implying that a diversity of bla CTX-M genetic environments exist. The ISEcp1 element is known to play an important role in bla CTX-M expression and gene transfer and is frequently located upstream of bla CTX-M genes (18,36,37). Surprisingly, such an element was not detected upstream of the bla CTX-M gene in these conjugative plasmids.…”

Section: Discussionmentioning

confidence: 82%

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes

Xie

Lin

Chen

et al. 2016

Antimicrob Agents Chemother

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A total of 55 cefotaxime-resistant Escherichia coli isolates were obtained from retail meat products purchased in Shenzhen, China, during the period November 2012 to May 2013. Thirty-seven of these 55 isolates were found to harbor a bla CTX-M gene, with the bla CTX-M-1 group being the most common type. bla CMY-2 was detected in 16 isolates, alone or in combination with other extended-spectrum ␤-lactamase (ESBL) determinants. Importantly, the fosA3 gene, which encodes fosfomycin resistance, was detected in 12 isolates, with several being found to reside in the conjugative plasmid that harbored the bla CTX-M gene. The insertion sequence IS26 was observed upstream of some of the bla CTX-M-55 and fosA3 genes. Conjugation experiments showed that bla CTX-M genes from 15 isolates were transferrable, with Inc I1 and Inc FII being the most prevalent replicons. High clonal diversity was observed among the bla CTX-M producers, suggesting that horizontal transfer of the bla CTX-M genes among E. coli strains in retail meats is a common event and that such strains may constitute an important reservoir of bla CTX-M genes, which may be readily disseminated to other potential human pathogens.

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Section: Discussionmentioning

confidence: 82%

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes

Xie

Lin

Chen

et al. 2016

Antimicrob Agents Chemother

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“…Fecal colonization by ESBL-producing E. coli in healthy individuals has been previously detected in Spain, with rates from 3.7% to 7.7% (10,11,12). A higher prevalence was found among the household contacts of patients infected with ESBLPE (12,13).…”

mentioning

confidence: 80%

High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

Fernández‐Reyes

Vicente

Gomariz

et al. 2014

Antimicrob Agents Chemother

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The prevalence of extended-spectrum-␤-lactamase-producing Enterobacteriaceae (ESBLPE) was studied in stool samples from 125 8-to 16-month-old healthy children. Twenty-four percent of them and 10.7% of the 318 fecal samples studied yielded extended-spectrum-␤-lactamase-producing Escherichia coli, with the types being SHV-12, CTX-M-1, CTX-M-14, and TEM-52, the most common types of ␤-lactamases. This high prevalence of ESBLPE in healthy people, which is to our knowledge the highest currently reported in Europe, may represent a risk for increased infections by these organisms in the future.

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“…All these strains were classified as Escherichia coli 8 . The authors of another Spanish study published in 2007 focused on assessing the prevalence of GIT colonization by ESBL-positive Enterobacteriaceae in a group of healthy persons and found 7% prevalence, with bacteria producing CTX-M enzymes being most frequent 26 . Janvier et al compared the prevalence of ESBL-positive Enterobacteriaceae in asymptomatic young subjects in the community over a period of 10 years (1999-2009) to detect an increase from 0% to 2%.…”

Section: Discussionmentioning

confidence: 99%

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Husičková¹,

Čekanová²,

Chromá³

et al. 2012

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aim. The study aimed at analyzing ESBL-and AmpC-positive Enterobacteriaceae in the gastrointestinal tracts of university hospital inpatients and persons from the Olomouc Region community, and comparing the results with data from 2007. Methods. Bacteria were isolated from rectal swabs inoculated onto the ChromID TM ESBL selective medium (bioMérieux). Production of ESBL-type beta-lactamases was confirmed by the modified double-disk synergy test and AmpC enzyme production was detected by the AmpC disk test. ESBL-and AmpC-positive isolates were subjected to basic genetic analysis aimed at detecting the bla TEM , bla SHV , bla CTX-M and bla AmpC genes. Results. Over the study period (1 March 2010 -1 May 2010), a total of 1,279 rectal swabs (70.4% of community subjects) were analyzed on the above medium. The prevalence rates of ESBL-positive Enterobacteriaceae were 8.2% in hospitalized patients and 3.2% in community subjects. Production of the AmpC enzyme was detected in 1.1% of bacterial isolates from the community and in one (0.3%) hospital isolate. Among ESBL, the most frequent genes encoding enzymes were from the CTX-M-1-like genes. Detected AmpC beta-lactamases belonged to the CIT, DHA and EBC groups. Conclusion. When compared with the year 2007, the rates of carriers of ESBL-positive bacteria increased in both hospitalized patients (from 3% to 8%) and community subjects (from 1% to 3%) in 2010. Given the fact that production of extended-spectrum beta-lactamases is clinically significant, knowing the epidemiological situation is very important for selecting adequate antibiotic therapy.

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Prevalence and diversity of extended-spectrum ß-lactamases in faecal Escherichia coli isolates from healthy humans in Spain

Cited by 79 publications

References 21 publications

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes

High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

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Prevalence and diversity of extended-spectrum ß-lactamases in faecal Escherichia coli isolates from healthy humans in Spain

Cited by 79 publications

References 21 publications

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla CTX-M and fosA3 Genes

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla CTX-M and fosA3 Genes

High Rate of Fecal Carriage of Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Healthy Children in Gipuzkoa, Northern Spain

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

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Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor bla _CTX-M and fosA3 Genes