Prevalence and diversity of carbapenemases among imipenem-nonsusceptible Acinetobacter isolates in Korea: emergence of a novel OXA-182

Kim, Changki; Lee, Yangsoon; Lee, Hyukmin; Woo, Gun-Jo; Song, Wonkeun; Kim, Mina; Lee, Wee-Gyo; Jeong, Seok Hoon; Lee, Kyungwon; Chong, Yunsop

doi:10.1016/j.diagmicrobio.2010.07.014

Cited by 44 publications

(25 citation statements)

References 21 publications

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“…1A]). OXA-182 was detected in South Korea using bla OXA-143-like primers (26). Although OXA-253 and OXA-255 share similar amino acid identity with OXA-182 and OXA-143, they differ in their numbers of identical and positive (including conservative substitutions) amino acids.…”

Section: Resultsmentioning

confidence: 99%

Characterization of bla _OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii

Zander

Bonnin

Seifert

et al. 2014

Antimicrob Agents Chemother

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The acquired carbapenem-hydrolyzing oxacillinase (OXA) OXA-143 has thus far been detected only in Acinetobacter baumannii isolates from Brazil. The aim of this study was to characterize three OXA-143 variants: OXA-231 and OXA-253 from carbapenemresistant A. baumannii isolates and OXA-255 in a carbapenem-susceptible Acinetobacter pittii isolate originating from Brazil, Honduras, and the United States, respectively. The 5= rapid amplification of cDNA ends (RACE) technique identified the same transcription initiation site for all bla OXA-143-like genes and revealed differences in the putative promoter regions. However, all cloned OXA-143 variants conferred carbapenem resistance on A. baumannii ATCC 17978 and OXA-255 conferred carbapenem resistance on A. pittii SH024, which was correlated with bla OXA-255 gene expression. This is the first description of OXA-143-like outside A. baumannii. Detection of OXA-143-like in the United States and Honduras indicates its dissemination through the American continent.

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Section: Resultsmentioning

confidence: 99%

Characterization of bla _OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii

Zander

Bonnin

Seifert

et al. 2014

Antimicrob Agents Chemother

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“…While bla OXA-23 is the main mechanism of imipenem resistance in A. baumannii, especially in Korea (Park et al, 2009d;Kim et al, 2010), A. baumannii isolates possessing bla IMP and bla VIM are also important contributors to imipenem resistance, but such isolates were not included in this study. Our study did not analyse the colistin and tigecycline resistance mechanisms for the A. baumannii isolates tested.…”

Section: K R Peck and Othersmentioning

confidence: 99%

In vitro time-kill studies of antimicrobial agents against blood isolates of imipenem-resistant Acinetobacter baumannii, including colistin- or tigecycline-resistant isolates

Peck

Kim

Choi

et al. 2012

Journal of Medical Microbiology

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The emergence of colistin or tigecycline resistance as well as imipenem resistance in Acinetobacter baumannii poses a great therapeutic challenge. The bactericidal and synergistic effects of several combinations of antimicrobial agents against imipenem-, colistin-or tigecyclineresistant A. baumannii isolates were investigated by in vitro time-kill experiments. Six imipenemresistant A. baumannii blood isolates were examined in this study, including colistin-and tigecycline-susceptible, colistin-resistant but tigecycline-susceptible, and colistin-susceptible but tigecycline-resistant isolates. Time-kill studies were performed using five antimicrobial agents singly or in combinations (imipenem plus colistin, imipenem plus ampicillin-sulbactam, colistin plus rifampicin, colistin plus tigecycline, and tigecycline plus rifampicin) at concentrations of 0.5¾ and 1¾ their MICs. Only imipenem was consistently effective as a single agent against all six A. baumannii isolates. Although the effectiveness of combinations of 0.5¾ MIC antimicrobial agents was inconsistent, combination regimens using 1¾ MIC of the antimicrobial agents displayed excellent bactericidal activities against all six A. baumannii isolates. Among the combinations of 0.5¾ MIC antimicrobial agents, the combination of colistin and tigecycline showed synergistic or bactericidal effects against four of the isolates. This in vitro time-kill analysis suggests that antimicrobial combinations are effective for killing imipenem-resistant A. baumannii isolates, even if they are simultaneously resistant to either colistin or tigecycline. However, the finding that the combinations of 0.5¾ MIC antimicrobial agents were effective on only some isolates may warrant further investigation of the doses of combination agents needed to kill resistant A. baumannii.

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“…Although OXA-23-like is the main subclass of carbapenemases identified in A. baumannii, IMP-4 is occasionally identified in A. pittii in locations such as Hong Kong and Singapore (18,28). Other variants of bla IMP , such as bla IMP-1 , bla IMP-8 , bla , and bla IMP-19 , have been described in A. pittii in Southeast Asia (16,29,30). A. pittii has also recently been reported to produce NDM (31,32).…”

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confidence: 99%

A Case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-Producing Acinetobacterpittii Sequence Type 119 in Australia

et al. 2015

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An IMP-4-producing Acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. Identification to the species level by rpoB and gyrB sequencing and multiplex-PCR-based analysis revealed that the isolate was A. pittii. Whole-genome sequencing of this A. pittii isolate determined the presence of bla OXA-96 , bla CARB-2 , and a novel bla OXA-421 gene. The position of this novel bla OXA-421 gene was similar to that of bla OXA-51 in A. baumannii, downstream of the phosphinothricin N-acetyltransferase gene and upstream of fxsA in the chromosome. This A. pittii isolate was found to belong to sequence type 119 (ST119). Here, we report the first isolation of IMP-4-producing A. pittii ST119 with a novel bla OXA-421 gene from a patient in Australia and characterize its draft genome. CASE REPORTA 67-year-old diabetic woman suffered a fall leading to a displaced distal spiral tibial plateau fracture. In the weeks prior to the fall, she had received multiple antimicrobials (clindamycin, lincomycin, cephalexin, ciprofloxacin, and ceftazidime) for an infected hematoma of the breast and a series of lower respiratory tract infections. The patient underwent definitive repair of the fracture but postoperatively developed osteomyelitis. Debridement of the leg wound was performed. Acinetobacter species and vancomycin-resistant Enterococcus strains were isolated from the tissue removed. This Acinetobacter species (CR12-42) was carbapenem resistant. Despite ongoing antibiotic treatment, the patient's leg required amputation in March 2013, after continuous inflammation, infections for more than 5 months, and an episode of severe Clostridium difficile infection resulting in colectomy. The leg infection was resolved by the amputation.The initial identification of this Acinetobacter species was done by Vitek 2. Antimicrobial susceptibility testing by Vitek 2 (bioMérieux) showed resistance to carbapenems, ceftazidime, ceftriaxone, cefepime, gentamicin, tobramycin, trimethoprimsulfamethoxazole, ticarcillin-clavulanic acid, and ciprofloxacin according to the EUCAST standard (1). The isolate was referred to our laboratory at the University of Queensland Centre for Clinical Research. The Acinetobacter isolate was identified to the species level by a gyrB multiplex PCR, which revealed that CR12-42 was Acinetobacter pittii (2). Partial rpoB sequencing (3) confirmed that CR12-42 was A. pittii.Phenotypic characterization to determine the class of carbapenemase was performed as previously described (4-6). The A. pittii isolate showed a metallo-␤-lactamase phenotype by producing a larger inhibition zone around carbapenem disks with EDTA than around carbapenem disks alone (Ͼ5-mm breakpoint increase in the size of the inhibition zone). The isolate also produced a positive result in the modified Hodge and Carba NP tests for carbapenemase production. MICs were determined with Etest (bioMérieux). The isolate was resistant to all of the carbapenems tested, i.e., ceftazidime, cefotaxime, cefepime, cefo...

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Prevalence and diversity of carbapenemases among imipenem-nonsusceptible Acinetobacter isolates in Korea: emergence of a novel OXA-182

Cited by 44 publications

References 21 publications

Characterization of bla _OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii

Characterization of bla _OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii

In vitro time-kill studies of antimicrobial agents against blood isolates of imipenem-resistant Acinetobacter baumannii, including colistin- or tigecycline-resistant isolates

A Case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-Producing Acinetobacterpittii Sequence Type 119 in Australia

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Prevalence and diversity of carbapenemases among imipenem-nonsusceptible Acinetobacter isolates in Korea: emergence of a novel OXA-182

Cited by 44 publications

References 21 publications

Characterization of bla OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii

Characterization of bla OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii

In vitro time-kill studies of antimicrobial agents against blood isolates of imipenem-resistant Acinetobacter baumannii, including colistin- or tigecycline-resistant isolates

A Case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-Producing Acinetobacterpittii Sequence Type 119 in Australia

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Characterization of bla _OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii

Characterization of bla _OXA-143 Variants in Acinetobacter baumannii and Acinetobacter pittii