Prevalence and Distribution of Rotavirus Genotypes Among Children With Acute Gastroenteritis in Areas Other Than Java Island, Indonesia, 2016–2018

Wahyuni, Rury Mega; Utsumi, Takako; Dinana, Zayyin; Yamani, Laura Navika; Juniastuti, Juniastuti; Wuwuti, Ishak Samuel; Fitriana, Elsa; Gunawan, Emily; Liang, Yujiao; Ramadhan, Fitratul; Soetjipto,; Lusida, Maria Inge; Shoji, Ikuo

doi:10.3389/fmicb.2021.672837

Cited by 11 publications

(6 citation statements)

References 38 publications

(55 reference statements)

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“…Several publications have reported the emergence of the G9 genotype in other countries during approximately the same period. A study performed in 2018 in the West Nusa Tenggara region of neighboring Indonesia detected the presence of G9P[8] strains; however, these strains were absent in South Sumatra and West Papua [ 32 ], indicating a localized emergence of this genotype. Furthermore, a study carried out in 2017–2018 in China showed G9P[8] to be the predominant strain [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Increase in rotavirus prevalence with the emergence of genotype G9P[8] in replacement of genotype G12P[6] in Sabah, Malaysia

et al. 2023

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Rotaviruses are major causative agents of acute diarrhea in children under 5 years of age in Malaysia. However, a rotavirus vaccine has not been included in the national vaccination program. To date, only two studies have been carried out in the state of Sabah, Malaysia, although children in this state are at risk of diarrheal diseases. Previous studies showed that 16%–17% of cases of diarrhea were caused by rotaviruses and that equine-like G3 rotavirus strains are predominant. Because the prevalence of rotaviruses and their genotype distribution vary over time, this study was conducted at four government healthcare facilities from September 2019 through February 2020. Our study revealed that the proportion of rotavirus diarrhea increased significantly to 37.2% (51/137) after the emergence of the G9P[8] genotype in replacement of the G12P[8] genotype. Although equine-like G3P[8] strains remain the predominant rotaviruses circulating among children, the Sabahan G9P[8] strain belonged to lineage VI and was phylogenetically related to strains from other countries. A comparison of the Sabahan G9 strains with the G9 vaccine strains used in the RotaSiil and Rotavac vaccines revealed several mismatches in neutralizing epitopes, indicating that these vaccines might not be effective in Sabahan children. However, a vaccine trial may be necessary to understand the precise effects of vaccination.

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Section: Discussionmentioning

confidence: 99%

Increase in rotavirus prevalence with the emergence of genotype G9P[8] in replacement of genotype G12P[6] in Sabah, Malaysia

et al. 2023

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“…This is different from previous reports, which identified Rotavirus as the most common pathogen in HIV-negative children with acute diarrhea [ 12 13 ]. The prevalence of pediatric diarrhea caused by Rotavirus infection remains high in Indonesia particularly in children <5 years old [ 14 ]. The absence of a national program for Rotavirus vaccine along with the presence of mostly HIV-negative subjects in this study (under 6 years old) explain the supposedly high prevalence of diarrhea associated with Rotavirus infection.…”

Section: Discussionmentioning

confidence: 99%

Detection of Enteropathogens in Human Immunodeficiency Virus and Non-Human Immunodeficiency Virus-Infected Children with Acute Diarrhea in an Indonesian Tertiary Hospital Using Multiplex Real-Time Polymerase Chain Reaction

Wulandari,

Febrian,

Dwipoerwantoro

et al. 2024

Pediatr Gastroenterol Hepatol Nutr

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Purpose Diarrhea is one of the leading causes of mortality in children living in developing countries. The etiology of acute diarrhea in each healthcare center varies depending on place, time, and population. This study aimed to identify pathogen patterns in human immunodeficiency virus (HIV)-infected and non-HIV children suffering from acute diarrhea, using multiplex real time reverse transcriptase polymerase chain reaction (RT-PCR), in an Indonesian tertiary hospital. Methods This cross-sectional study was conducted at Dr. Cipto Mangunkusumo National Hospital from March 2019 to April 2020. Results The study showed that multiplex RT-PCR results were positive in 58.9% of the specimens, with more positive results in HIV-infected children than in non-HIV-infected children (70% vs. 54.7%). Altogether 72 enteropathogens were detected from all specimens. Enteropathogens in non-HIV children with acute diarrhea consisted of bacteria (70.6%) and viruses (29.4%) with a predominance of enteroaggregative Escherichia coli (25.4%), followed by Campylobacter spp. (11.8%), enteropathogenic E. coli (9.8%), Norovirus GII (7.8%), and Clostridium difficile (7.8%). Enteropathogens in HIV-infected children consisted of viruses (57.1%), bacteria (28.6%), and parasites (14.3%) comprising Norovirus GII (24%), Cryptosporidium spp. (14.3%), Campylobacter spp. (14.3%), Norovirus GI (14.3%), and Astrovirus (14.3%). Cryptosporidium spp. was the only parasite found in this study and was found only in HIV-infected children. In non-HIV children with acute diarrhea, most pathogens were invasive bacteria, while in HIV-infected children, more viral and parasite infections occurred, primarily caused by opportunistic pathogens. Conclusion The pattern of enteropathogens can help clinicians determine further examinations and appropriate empirical antimicrobial therapy for the patient.

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“…The expressions of HBcAg recombinant protein in bacteria [18][19][20] and yeast [21][22][23] have been reported in various studies. In this study, we report the expression of HBcAg protein derived from HBV sub-genotype B3, one of the predominant sub-genotypes in Indonesia [24][25][26][27][28], in Lactococcus lactis.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of HBcAg Using Food Grade Vector pNZ8149 in Lactococcus Lactis for the Development of HBV Therapeutic Vaccine

Umami¹,

Anwar²,

Murtiyaningsih³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Hepatitis B virus (HBV) infection remains a major cause of chronic liver diseases which may develop into cirrhosis and hepatocellular carcinoma (HCC). Although HBV prophylactic vaccine is available, currently there is no complete cure for chronic hepatitis B (CHB). One of the alternatives for CHB treatment is therapeutic vaccine. The HBV core antigen (HBcAg) becomes a suitable candidate for HBV therapeutic vaccine since it is known as a potent inducer of B-cell and T-cell responses. In this study, we report the development of HBV therapeutic vaccine by expressing HBcAg derived from HBV subgenotype B3, which is one of the predominant sub-genotypes in Indonesia. A food grade expression vector pNZ8149 which contains nisin-controlled gene as an inducible promoter, was used to express HBcAg in Lactococcus lactis NZ3900. Moreover, signal peptide usp45 was added in the N-terminal of the gene of interest to increase protein secretion efficiency. The native HBV core gene was synthesized and successfully cloned into plasmid pNZ8149 and transformed into Lactococcus lactis NZ3900 using lactose as a selection marker. The expression of HBcAg was induced using 10 ng/mL nisin and confirmed using dot blot hybridization analysis. Finally, the target protein which is 21 kDa in size was obtained by partial purification using size exclusion chromatography. In conclusion, HBcAg was successfully cloned and expressed using a food grade expression vector pNZ8149 in Lactococcus lactis NZ3900. These findings will pave the development of therapeutic vaccine for CHB treatment in the future.

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Prevalence and Distribution of Rotavirus Genotypes Among Children With Acute Gastroenteritis in Areas Other Than Java Island, Indonesia, 2016–2018

Cited by 11 publications

References 38 publications

Increase in rotavirus prevalence with the emergence of genotype G9P[8] in replacement of genotype G12P[6] in Sabah, Malaysia

Increase in rotavirus prevalence with the emergence of genotype G9P[8] in replacement of genotype G12P[6] in Sabah, Malaysia

Detection of Enteropathogens in Human Immunodeficiency Virus and Non-Human Immunodeficiency Virus-Infected Children with Acute Diarrhea in an Indonesian Tertiary Hospital Using Multiplex Real-Time Polymerase Chain Reaction

Cloning and Expression of HBcAg Using Food Grade Vector pNZ8149 in Lactococcus Lactis for the Development of HBV Therapeutic Vaccine

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