Prevalence and distribution of human herpesvirus 7 in normal brain

Chan, Paul K.S.; Ng, Ho Keung; Cheung, Jo L.K.; Ng, King Cheung; Cheng, A. F. B.

doi:10.1002/1096-9071(200011)62:3<345::aid-jmv6>3.0.co;2-#

Cited by 33 publications

(27 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oligonucleotide primers have been described previously. 18,19 All of the DNA samples were tested for suitability for amplification using primers specific for a fragment of the human b-hemoglobin gene (primers PC03/KM38). Only specimens from which cellular b-globin gene sequences were amplified were then examined by PCR amplification using primers KS-1, 5 0 -AGC CGA AAG GAT TCC ACC AT-3 0 and KS-2, 5 0 -TTC GTG TTG TCT ACG TCC AG-3 0 targeting a 233-bp fragment of the open reading frame 26.…”

Section: Polymerase Chain Reaction Amplification Methodsmentioning

confidence: 99%

“…Only specimens from which cellular b-globin gene sequences were amplified were then examined by PCR amplification using primers KS-1, 5 0 -AGC CGA AAG GAT TCC ACC AT-3 0 and KS-2, 5 0 -TTC GTG TTG TCT ACG TCC AG-3 0 targeting a 233-bp fragment of the open reading frame 26. 18 Primers were obtained from Integrated DNA Technologies, Inc. (Coralville, IA, USA). The positive control was a plasmid DNA containing cloned viral sequences of the HHV-8 capsid antigen gene.…”

Section: Polymerase Chain Reaction Amplification Methodsmentioning

confidence: 99%

“…High-stringency annealing temperatures specific for each primer set have been described elsewhere. 18,19 PCR amplification products were analyzed by agarose gel electrophoresis following standard protocols.…”

Section: Polymerase Chain Reaction Amplification Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Plasmablastic lymphomas and plasmablastic plasma cell myelomas have nearly identical immunophenotypic profiles

Vega

Chang

Medeiros³

et al. 2005

Modern Pathology

318

299

View full text Add to dashboard Cite

Plasmablastic lymphoma is an aggressive neoplasm that shares many cytomorphologic and immunophenotypic features with plasmablastic plasma cell myeloma. However, plasmablastic lymphoma is listed in the World Health Organization (WHO) classification as a variant of diffuse large B-cell lymphoma. To characterize the relationship between plasmablastic lymphoma and plasmablastic plasma cell myeloma, we performed immunohistochemistry using a large panel of B-cell and plasma cell markers on nine cases of plasmablastic lymphoma and seven cases of plasmablastic plasma cell myeloma with and without HIV/AIDS. The expression profiles of the tumor suppressor genes p53, p16, and p27, and the presence of Epstein-Barr virus (EBV) and human herpes virus type 8 (HHV-8) were also analyzed. All cases of plasmablastic lymphoma and plasmablastic plasma cell myeloma were positive for MUM1/IRF4, CD138, and CD38, and negative for CD20, corresponding to a plasma cell immunophenotype. PAX-5 and BCL-6 were weakly positive in 2/9 and 1/5 plasmablastic lymphomas, and negative in all plasmablastic plasma cell myelomas. Three markers that are often aberrantly expressed in cases of plasma cell myelomas, CD56, CD4 and CD10, were positive in 5/9, 2/5, and 6/9 plasmablastic lymphomas, and in 3/7, 1/5, and 2/7 plasmablastic plasma cell myelomas. A high Ki-67 proliferation index, overexpression of p53, and loss of expression of p16 and p27 were present in both tumors. No evidence of HHV-8 infection was detected in either neoplasm. The only significant difference between plasmablastic lymphoma and plasma cell myeloma was the presence of EBV-encoded RNA, which was positive in all plasmablastic lymphoma cases tested and negative in all plasma cell myelomas. In conclusion, most cases of AIDS-related plasmablastic lymphoma have an immunophenotype and tumor suppressor gene expression profile virtually identical to plasmablastic plasma cell myeloma, and unlike diffuse large B-cell lymphoma. These results do not support the suggestion in the WHO classification that plasmablastic lymphoma is a variant of diffuse large B-cell lymphoma.

show abstract

Section: Polymerase Chain Reaction Amplification Methodsmentioning

confidence: 99%

Section: Polymerase Chain Reaction Amplification Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Plasmablastic lymphomas and plasmablastic plasma cell myelomas have nearly identical immunophenotypic profiles

Vega

Chang

Medeiros³

et al. 2005

Modern Pathology

318

299

View full text Add to dashboard Cite

show abstract

“…Whereas Campylobacter jejuni is the commonest microorganism causing this condition, herpesviruses other than HHV-7 (namely CMV and EBV) have also been reported. 18 The interpretation of a positive PCR for HHV-7 DNA in CSF is complicated because the virus has been detected in normal brain tissue of 5% of adults who died of non-neurologic conditions 19 and HHV-7 establishes lifelong latency within lymphocytes (cells that are present in normal CSF albeit in small numbers). Lifelong latency carries with it the possibility of reactivation, but in our cohort, we found no serologic evidence for this (ie, in subjects who had paired sera there was no rising antibody titer of high avidity; data not (3) Enterovirus (1) c Enterovirus RNA detected in CSF by PCR M. tuberculosis (1)…”

Section: Discussionmentioning

confidence: 99%

Delayed Primary HHV-7 Infection and Neurologic Disease

Schwartz

Richardson²,

Ward

et al. 2014

Pediatrics

View full text Add to dashboard Cite

BACKGROUND: Primary human herpesvirus 7 (HHV-7) infection occurs almost universally during the first 5 years of life and is rarely accompanied by central nervous system (CNS) symptoms such as febrile seizures. The present retrospective study investigated the role of primary HHV-7 infection in CNS disease in children, including adolescents. METHODS: The study included all children who had neurologic disease aged younger than 18 years seen at the Hospital for Sick Children, Toronto, Canada, between April 1, 1998 and December 31, 2011, whose cerebrospinal fluid (CSF) was found by polymerase chain reaction to contain HHV-7 DNA. Where sera were available, HHV-7 IgG antibody titers and avidity were measured to differentiate primary from past infection. RESULTS: HHV-7 DNA was detected in the CSF of 57 (1.9%) of the 2972 children tested. In 3 adolescents primary HHV-7 infection (low avidity IgG) was confirmed as the cause of neurologic disease, 2 who had encephalitis and 1 who had Guillain-Barré syndrome. Eighteen children had possible HHV-7 disease (no alternative cause identified and indeterminate antibody result or serum not available), 7 encephalitis, 8 meningitis, and 3 demyelinating disorders. HHV-7 disease was excluded in 36 children on the basis of past infection (high IgG avidity) and/or an alternative cause. CONCLUSIONS: Primary HHV-7 infection delayed into adolescence can cause serious neurologic disease. HHV-7 DNA in CSF alone is insufficient to prove an etiologic association. Combining CSF polymerase chain reaction with serology is essential to prove primary infection when investigating HHV-7 CNS disease.

show abstract

“…However, we believe that this is unlikely as we did not detect HHV-7 DNA in the patient's serum or peripheral blood leukocytes. On the other hand, HHV-7 has been detected in normal brain tissue 16,17 and it is therefore probable that HHV-7 DNA in the patient's CSF had reactivated from latency within the CNS. Indeed, the patient was already infected with HHV-7 prior to transplant as indicated by specific IgG antibodies of high avidity.…”

Section: Discussionmentioning

confidence: 99%

Human herpesvirus-7 infection of the CNS with acute myelitis in an adult bone marrow recipient

Ward

White

Mackinnon

et al. 2002

Bone Marrow Transplant

View full text Add to dashboard Cite

Summary:The ␤-herpesviruses, human herpesviruses-6 and -7 (HHV-6 and HHV-7), are closely related and have very similar biological behaviour. While HHV-6 is associated with encephalitis in immunosuppressed adults, HHV-7 is not recognised as a cause of neurological disease in such patients. This report describes the identification of a reactivated HHV-7 infection in the cerebrospinal fluid of an adult who presented with an acute myelitis 11 months after unrelated donor bone marrow transplant. Bone Marrow Transplantation (2002) 30, 983-985.

show abstract

Prevalence and distribution of human herpesvirus 7 in normal brain

Cited by 33 publications

References 31 publications

Plasmablastic lymphomas and plasmablastic plasma cell myelomas have nearly identical immunophenotypic profiles

Plasmablastic lymphomas and plasmablastic plasma cell myelomas have nearly identical immunophenotypic profiles

Delayed Primary HHV-7 Infection and Neurologic Disease

Human herpesvirus-7 infection of the CNS with acute myelitis in an adult bone marrow recipient

Contact Info

Product

Resources

About