Prevalence and distribution of HHV-8 in different subpopulations, with and without HIV infection, in Spain

Gambús, Gemma; Bourboulia, Dimitra; Esteve, Anna; Lahoz, Rebeca; Rodriguez, C.; Bolao, Ferrán; Sirera, Guillem; Muga, Roberto; Romero, Jorge del; Boshoff, Chris; Whitby, Denise; Casabona, Jordi

doi:10.1097/00002030-200106150-00012

Cited by 57 publications

(37 citation statements)

References 41 publications

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“…A bias in our estimates could take place if the underlying HHV-8 prevalence of the control population could affect hospitalisation rates due to HHV-8-related diseases. Available data from the Spanish general population, however, indicate that the HHV-8 prevalence ranges between 6 and 8% (Gambus et al, 2001;de Sanjose et al, 2002), which is similar to the 5.3% observed in our control subjects, and also conforms to the moderate prevalence observed in Mediterranean countries. Owing to small numbers in some lymphoma categories, we cannot exclude that some of the results are due to chance.…”

Section: Discussionsupporting

confidence: 90%

Risk of malignant lymphoma associated with human herpesvirus-8: a case–control study in Spain

et al. 2004

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Section: Discussionsupporting

confidence: 90%

Risk of malignant lymphoma associated with human herpesvirus-8: a case–control study in Spain

et al. 2004

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“…Slides were then washed in blocking buffer, and fluorescein isothiocyanateconjugated rabbit anti-human immunoglobulin G (IgG) antibody (P0214; DAKO A/S, Denmark), diluted at 1:60 in the blocking solution, was added. Following incubation at 22°C for 22 min, slides were washed in PBS and examined under a fluorescence microscope for nuclear punctate staining (15).…”

Section: Methodsmentioning

confidence: 99%

“…In the absence of a culture system derived from KS tumorassociated cell lines to allow direct demonstration of KSHV (2,18), the diagnosis of KSHV infection has relied on molecular techniques and the use of serological assays to detect antibodies to KSHV antigens produced during latent or lytic phases of the viral life cycle (13,15,26). The immunofluorescence assay to detect anti-LANA antibodies (IFA-LANA) has long been considered a reference serological test to detect KSHV infection (23).…”

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confidence: 99%

Comparative Study of Kaposi's Sarcoma-Associated Herpesvirus Serological Assays Using Clinically and Serologically Defined Reference Standards and Latent Class Analysis

et al. 2007

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Accurate determination of infection with Kaposi's sarcoma-associated herpesvirus (KSHV) has been hindered by the lack of a "gold standard" for comparison of serological assays used to estimate KSHV prevalence in serosurveys conducted in different settings. We have evaluated the performance of five in-house (developed at University College London [UCL], United Kingdom, and at the virology laboratory of the Instituto de Medicine Tropical [IMT] in Sao Paulo, Brazil) and two commercial (ABI and DIAVIR) serological assays to detect antibodies to latency-associated nuclear antigen (LANA) and to lytic KSHV antigens. We used a variety of serum samples assembled to represent populations likely to be at high, intermediate, and low risk of KSHV infection in Brazil. Composite reference standard panels were prepared based on clinical and serological parameters, against which assay performances were assessed using conventional Bayesian statistics and latent class analysis (LCA). Against the clinical reference standard, in-house immunofluorescence assays to detect anti-LANA antibodies (IFA-LANA) produced at UCL and IMT had similar performances, with sensitivities of 61% (95% confidence interval [CI], 48% to 74%) and 72% (95% CI, 58% to 83%) and specificities of 99% (95% CI, 94% to 100%) and 100% (95% CI, 96% to 100%), respectively, and only the IMT IFA-LANA was included in LCA, together with the IMT IFA-lytic and four enzyme-linked immunosorbent assays (ELISAs). The LCA indicated that the IMT whole-virus ELISA performed best (sensitivity, 87% [95% CI, 81% to 91%]; and specificity, 100% [95% CI, 98% to 100%]), confirming the results obtained with the conventional statistical approach. Commercially available ELISA-based tests yielded the lowest specificities using a spectrum of serum samples. The evaluation of KSHV serological assays is warranted before planning serosurveys in various settings.

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“…However, hot spots also occur in low-incidence regions: in the Po Valley in northern (mainland) Italy, the incidence is 2.5/100,000 men and 0.7/100,000 women, with one rural zone having double the male rate and four times the female rate (24). Elsewhere in Mediterranean Europe, a group of blood donors in Spain had a low KSHV seropositivity similar to that of northern Italians (171). The high prevalence of KSHV in the Mediterranean contrasts with non-Mediterranean European countries such as Latvia, where a recent screening of 150 healthy blood donors by PCR detected human cytomegalovirus, HHV-6, and HHV-7 but failed to detect KSHV (269).…”

Section: Classic Ksmentioning

confidence: 99%

Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis

Dourmishev

Palmeri

et al. 2003

Microbiol Mol Biol Rev

309

367

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Kaposi's sarcoma had been recognized as unique human cancer for a century before it manifested as an AIDS-defining illness with a suspected infectious etiology. The discovery of Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus-8, in 1994 by using representational difference analysis, a subtractive method previously employed for cloning differences in human genomic DNA, was a fitting harbinger for the powerful bioinformatic approaches since employed to understand its pathogenesis in KS. Indeed, the discovery of KSHV was rapidly followed by publication of its complete sequence, which revealed that the virus had coopted a wide armamentarium of human genes; in the short time since then, the functions of many of these viral gene variants in cell growth control, signaling apoptosis, angiogenesis, and immunomodulation have been characterized. This critical literature review explores the pathogenic potential of these genes within the framework of current knowledge of the basic herpesvirology of KSHV, including the relationships between viral genotypic variation and the four clinicoepidemiologic forms of Kaposi's sarcoma, current viral detection methods and their utility, primary infection by KSHV, tissue culture and animal models of latent- and lytic-cycle gene expression and pathogenesis, and viral reactivation from latency. Recent advances in models of de novo endothelial infection, microarray analyses of the host response to infection, receptor identification, and cloning of full-length, infectious KSHV genomic DNA promise to reveal key molecular mechanisms of the candidate pathogeneic genes when expressed in the context of viral infection

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Prevalence and distribution of HHV-8 in different subpopulations, with and without HIV infection, in Spain

Cited by 57 publications

References 41 publications

Risk of malignant lymphoma associated with human herpesvirus-8: a case–control study in Spain

Risk of malignant lymphoma associated with human herpesvirus-8: a case–control study in Spain

Comparative Study of Kaposi's Sarcoma-Associated Herpesvirus Serological Assays Using Clinically and Serologically Defined Reference Standards and Latent Class Analysis

Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis

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