Prevalence and Distribution of BK virus Subtypes in Renal Transplant Recipients Referred to Golestan Hospital in Ahvaz, Iran

Kaydani, Gholam Abbas; Makvandi, Manoochehr; Samarbafzadeh, Alireza; Shahbazian, Heshmatollah; Hamidi-Fard, Mojtaba

doi:10.5812/jjm.16738

Cited by 21 publications

(19 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BKV Ib-2 is the only detectable genotype in our study cohort from Iran. The predominant BKV subtype Ib-2 in this study is in accordance with previous surveys from Iranian, European and American populations [18,19].…”

Section: Resultssupporting

confidence: 93%

See 1 more Smart Citation

Frequency and subtype of BK virus infection in Iranian patients infected with HIV

Akhgari

Mohraz

Azadmanesh

et al. 2015

Med Microbiol Immunol

View full text Add to dashboard Cite

Human polyomavirus BK virus (BKV) is a double-stranded DNA virus that infects approximately 90 % of the general population as a subclinical or mild infection. In immunosuppressed patients, such as HIV cases, BKV may be reactivated resulting hemorrhagic cystitis and tubulointerstitial nephritis. However, there are limited studies on prevalence and molecular epidemiology of BKV in Iran. We therefore aimed to evaluate the prevalence and subtypes of BKV in Iranian HIV patients. A total of 99 patients with HIV infection were enrolled in the study. Presence of BKV DNA in plasma was evaluated by nested PCR. PCR products were sequenced directly, and phylogenetic analysis was performed. BKV DNA was detected in 8.08 % of HIV patients. BKV viremia presented in 4 out of 25 patients (16 %) not receiving antiretroviral therapy in comparison with 4 out 74 of HAART-treated patients (5.4 %) (P = 0.023). In patients with CD4 counts ≥200 cells/mm(3), viremia was found more commonly (7/80 = 8.8 %) than in those with lower counts (1/19 = 5.2 %) (not significant). All sequenced BKV isolates belonged to subtype Ib-2. Our findings indicated that the prevalence of BKV viremia is relatively prevalent in patients with HIV infection and significantly higher in naïve than HAART-treated cases. Therefore, HAART can eliminate BKV infection from plasma and reduce viremia although the actual implication of BKV viremia in HIV patients is not clear.

show abstract

Section: Resultssupporting

confidence: 93%

“…Few studies carried out on BKV prevalence in dialysis patients and kidney transplant recipients in Iran [19,20]. However, there are insufficient data on the prevalence and molecular epidemiology of BKV viremia in HIV patients, and also the role of antiretroviral treatment in this setting has not been well clarified.…”

Section: Introductionmentioning

confidence: 98%

Frequency and subtype of BK virus infection in Iranian patients infected with HIV

Akhgari

Mohraz

Azadmanesh

et al. 2015

Med Microbiol Immunol

View full text Add to dashboard Cite

show abstract

“…A recent study from Iran reported 41.8% viruria. A study from Kuwait reported 45% viruria and 26% viraemia in a group of patients with allograft dysfunction [5,6]. In our study the rate of viruria was somewhat higher but the rate of viraemia was comparable.…”

Section: Discussioncontrasting

confidence: 46%

Prevalence of BK virus among renal transplant recipients in a tertiary care hospital in Sri Lanka

Premathilake¹,

Jayamaha²,

Lanerolle³

2018

Ceylon Med. J.

View full text Add to dashboard Cite

Introduction BK virus nephropathy (BKVN) is an important cause of graft failure in post renal transplant patients. Detection of BK virus replication early enables prevention of BK virus nephropathy. Understanding BK virus epidemiology in post renal transplant patients will be useful in implementing a routine screening programme. Objectives Objectives were to determine the prevalence of BK virus viruria and viraemia among post renal transplant patients within the first two years of transplantation. Methodology A hospital-based, descriptive crosssectional study was conducted on 136 clinic and inward patients. Plasma and urine were tested for BK virus DNA using real time PCR. Serum creatinine done within two weeks of data collection was recorded. Results The prevalence of BK virus viruria was 53.67% and viraemia was 11%. Viraemia >1000 copies/ml was associated with abnormal serum creatinine and higher median serum creatinine. No similar association was observed with viruria. Among patients with normal serum creatinine, virus was not detected in urine in 48.9% and plasma in 92.7%. Conclusions The prevalence of BK virus is high in this study population. Significant viraemia was associated with elevated serum creatinine. Viruria or viraemia was not detected among a large number of patients with normal serum creatinine.

show abstract

“…The virus can be found in people who are immunocompromised, such as people who have received organ transplants (especially kidney transplants), as well as pregnant women or people with AIDS, and will again be activated and causes illness. The virus has been isolated from urine of 10%-60% of people who have received kidney transplants and can develop nephropathy in some of these people (3,5,(7)(8)(9)(10)(11). The virus can cause hemorrhagic cystitis or urinary tract obstruction in people who have a bone marrow transplant (7,(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

“…The subtypes 1 have spread around the world, while subtype 4 can be seen only in East Asia. Moreover, subtypes 2 and 3 have been rarely reported in some areas (9,11,(24)(25)(26)(27)(28)(29). Usually multiple mutations occur in the VP1 gene, but sometimes gene mutations also occur in the TAg.…”

Section: Introductionmentioning

confidence: 99%

The complete genome sequence BK polyomavirus study in kidney transplanted patients

et al. 2018

Self Cite

View full text Add to dashboard Cite

Background: BK polyomavirus is a member of the Polyomaviridae. This virus has spread worldwide and up to 82% of the world populations are serologically positive. BK polyomavirus usually transmits through inhalation or fecal-oral way in childhood, as well as is likely to cause an asymptomatic disease. The virus can be reactivated in people who are immunocompromised. Objectives: In this study we aimed to determine the complete genome sequence of BK polyomavirus in 5 patients receiving kidney transplantation in Golestan hospital, Ahvaz, south west of Iran. Materials and Methods: The study was performed on urine and blood samples from kidney transplant ward, Golestan hospital, Ahvaz, Iran. To amplify the whole genome of BK polyomavirus AccuPower ProFi Taq PCR PreMix kit (Bioneer, South Korea) was used. Then, purified fragments were cloned into the vector. Sequences derived from the sequencing process were assembled by MEGA7 software. Data were analyzed using MEGA7 software and a phylogenetic tree was built based on the maximum likelihood ratio method. Results: Overall, 40 urine samples (40%) and two plasma samples (12%) were positive for BK virus DNA. For the selected samples, W.G. Long PCR was performed and 5.1 kbp fragment was observed in all five samples. These fragments were cloned and sequenced. Genomic sequence analysis of the 5 strains studied showed 97.6% homology and all our study samples were of the same clade, which could be a reason for our patients to be infected with the same strain. Comparing the 5 isolates in our study with reference strains, showed more than 98% of homology, and variation was observed in less than 2% of nucleotides. Conclusion: These five isolates showed more than 98% homology compared to the reference sequences, which can be attributed to the fact that these patients are infected with a common strain or that the genomic stability in the strain exists in our geographic area. We can conclude that in our geographic region there is a genomic stability and this strain can also be used as a positive control sample in other parts of the country.

show abstract

Prevalence and Distribution of BK virus Subtypes in Renal Transplant Recipients Referred to Golestan Hospital in Ahvaz, Iran

Cited by 21 publications

References 21 publications

Frequency and subtype of BK virus infection in Iranian patients infected with HIV

Frequency and subtype of BK virus infection in Iranian patients infected with HIV

Prevalence of BK virus among renal transplant recipients in a tertiary care hospital in Sri Lanka

The complete genome sequence BK polyomavirus study in kidney transplanted patients

Contact Info

Product

Resources

About