Prevalence and Cytotoxic Effects of Some Colibactin and <i>cnf</i> Genes among <i>Escherichia coli</i> Isolated from Urinary Tract Infections

Alhadidi, Hiba A.S.; Al-Qaysi, Safaa A. S.; Al-Halbosiy, Mohammad M. F.

doi:10.48022/mbl.2203.03005

Cited by 4 publications

(4 citation statements)

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“…Toluidine blue should only be used with Enterobacteriaceae since it may be harmful to some gram-positive cocci (17) . The results of (32) revealed that out of 110 isolates, 28 harbored the clbA gene, 40 harbored clb B, and 13 isolates harbored clbA, clbB, and cnf1 genes. The results of Hussein et al (3) showed that the distribution of pks+ among the collected clinical isolates showed that 7 isolates belonged to E. coli.…”

Section: Discussionmentioning

confidence: 99%

Detection of cdt and clb Genes in pathogenic E.coli

2023

The Egyptian Journal of Hospital Medicine

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Background: Many diseases create the cytolethal-distending toxin (CDT), which is representative of an emerging family of recently identified bacterial compounds. Objective: This study was performed to detect the antibiotic resistance properties and distribution of cdtB and clbA in E. coli strains isolated from urine and stool samples. Materials and Methods: The sensitivity test was evaluated by using different types of antibiotics in the current study, and the cdtB and clb genes were detected by PCR in all bacterial isolates. Results: The antibiotics sensitivity test show variable degrees of sensitivity and resistance. The high percentage of sensitivity was achieved against amikacin at a percentage of 86% and ciprofloxacin, ceftazidime, gentamicin, and tobramycin at a percentage of 80%, whereas trimethoprim and aztreonam at 64% and 60% respectively. In this study, most isolates were resistant to amoxicillin (92%); while showing different degrees of resistance against other types of antibiotics ranging from tetracycline (62%) to amikacin 4%. The frequency of MDR bacteria was about 64 % (32 isolates), 30 from urine, and 2 others from stool. The results showed that the clbA gene was found in 6 bacterial isolates (12%), whereas the other 44 (88%) isolates don't have this gene, while the cdtB gene wasn't found in any one of the bacterial isolates. Conclusions: E.coli was recorded as multidrug resistance (MDR) and the clb gene was found only in 6 bacterial isolates but it was not the cdtB gene detected in all isolates.

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Section: Discussionmentioning

confidence: 99%

Detection of cdt and clb Genes in pathogenic E.coli

2023

The Egyptian Journal of Hospital Medicine

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“…In addition to being isolated from sick and healthy animals like cats, dogs, pigs, and birds, E. coli strains that produce CNFs are also known to cause meningitis and UTIs in people (33). A study by Alhadidi et al (34) found that no cnf2 gene was found in any of the collected isolates, but 13 isolates out of 110 (11.8%) had the clbA, clbB, and cnf1 genes. These findings are in agreement with the current study.…”

Section: Table (1): Frequency Of Cnf1 and Cnf2 Genes In E Coli Isolat...mentioning

confidence: 99%

Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli

khalaf¹,

Flayyih²

2022

The Egyptian Journal of Hospital Medicine

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Background: CNF are single-chain proteins that are created by some uropathogenic Escherichia coli.There are three types of CNFs detected in E.coli, but CNF1 represent more frequently. Objective: The purposes of this study were to determine the prevalence of cnf1 and cnf2 genes in E. coli isolates by using specific primers. Materials and Methods: The sensitivity test was evaluated by using different types of antibiotics in the current study, and the cnf1 and cnf2 genes were detected by PCR in all bacterial isolates. Results: The antibiotics sensitivity test show variable degrees of sensitivity and resistance. The high percentage of sensitivity was achieved against amikacin at a percentage of 86% and ciprofloxacin, ceftazidime, gentamicin, and tobramycin at a percentage of 80%, whereas trimethoprim and aztreonam at 64%and 60% respectively. In this study, most isolates were resistant to Amoxicillin (92%); while showing different degrees of resistance against other types of antibiotics ranging from Tetracycline (62%) to amikacin 4%. The frequency of MDR bacteria was about 64 % (32 isolates), 30 from urine, and 2 others from stool. The results revealed that the cnf1 gene was found in Five bacterial isolates (10%), whereas the other 45 (90%) isolates don't have this gene, while cnf2 wasn't found in any one of the bacterial isolates. Conclusion: E.coli was recorded as multidrug resistance (MDR) and the cnf1 gene was found only in uropathogenic isolates.

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“…E. coli carries various virulence factors such as adhesins, secretory systems, and toxic proteins [26,27] . The chromosomally encoded cyclomodulin, called cytotoxic necrotizing factor 1 (CNF-1) and produced by UPEC and other pathogenic strains, is associated with bacterial colonization, virulence, invasion, survival, and distribution in the body, it is a leading cause of host cell cycle changes such as colorectal tumorigenesis, apoptosis, inflammation, and tissue damage [28][29][30][31][32] . The association of cnf-1 gene carriage and quinolone resistance or ESBL production has not been demonstrated previously.…”

Section: Introductionmentioning

confidence: 99%

“…Extended-... [13] Ortiz-Dí� ez G,et al Prevalence, incidence... [14] Lima LS,High genetic diversity... [15] Briales A, et al Prevalence of... [16] Kariuki K,et al Plasmid-mediated... [17] Jacoby GA, Plasmid... [18] Akshay SD, Differential... [19] Rodrí� guez-Martí� nez JM, Plasmid-mediated... [20] Aldred KJ, Mechanism of... [21] Doma AO,Comparative, ... [22] Amador P, Prevalence of... [23] Aworh MK, Quinolone-resistant ... [24] Ruiz E, et al qnr, aac(6')... [25] Mohamed Ali I, et al Microbiology and ... [26] Sora VM, Extraintestinal... [27] Raimondi S, Antibiotic,... [28] Wang MH,.Cytotoxic necrotizing... [29] Li XZ. Quinolone resistance... [30] Herrera-Vázquez A, Detection of... [31] Alhadidi HA, Prevalence and... [32] Chat H, Cytotoxic... [33] Harwalkar A, Lower prevalenc... [34] Da Silva GJ, Association between... [35] Marin J, et al The population... [36] Biggel M, Convergence of... [37] Jia Y, Study on... [38] Guiral E, Prevalence of... [39] Sfeir MM. Adoption of... [40] Quesada MD, et al Performance of... [41] Cattoir V, Multiplex PCR... [42] Azam MW, Updates on... [43] Akiyama T, Molecular... [44] Chong Y, Current epidemiology, ... [45] Onishi R, et al Impact on... [46] Rahn DD.…”

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confidence: 99%

Prevalence of Extended-Spectrum B-Lactamase (ESBL) and Quinolone Resistance (qnr) Genes among Cytotoxic Necrotizing Factor-1-Producing Uropathogenic Escherichia coli in Babylon, Iraq

Qasim Raheem,

Al-Maliki,

Alaa Abdolzahra

et al. 2023

IEM

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Background: Pathogenic Escherichia coli (E. coli) is usually known as the principal agent of hospital-acquired infections, particularly urinary tract infections (UTIs). This study aimed to determine ESBL (extended-spectrum B-lactamase) production and quinolone resistance (qnr) genes in cytotoxic necrotizing factor 1 (CNF-1)-producing E. coli isolates from UTIs in Iraq. Materials & Methods: A total of 996 E. coli isolates were obtained from UTIs in two general hospitals in Hillah, Babylon, Iraq (during 2014-2022), and 100 uropathogenic E. coli (UPEC) were cnf-1 carriers. ESBL production was evaluated using the doubledisk synergy test. qnr genes were detected using polymerase chain reaction (PCR). Findings: Nalidixic acid and chloramphenicol resistance was 70 and 30%, respectively. ESBL production was observed among 46% of cnf-1 carriers. qnrA, qnrB, and qnrS genes were detected in 18, 21, and 11% of the isolates, respectively. ESBL-producing isolates mainly carried the qnrB gene and showed the highest resistance levels to quinolones. Major risk factors of pathogenic E. coli isolation included older age (68%, p= .031), previous hospitalization (76%, p= .021), and urinary catheter (83%, p= .018). Conclusion:Although the prevalence of cnf-1 gene was not high among UPEC isolates, its prevalence was high among quinolone-resistant and ESBL-producing isolates. Continuous investigation of virulence and resistance genes is essential for monitoring and controlling infections. It is necessary to determine virulence factors and resistance genes among UPEC in Iraq and take timely measures to hinder the spread of resistance genes to other nosocomial isolates.

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Prevalence and Cytotoxic Effects of Some Colibactin and cnf Genes among Escherichia coli Isolated from Urinary Tract Infections

Cited by 4 publications

References 33 publications

Detection of cdt and clb Genes in pathogenic E.coli

Detection of cdt and clb Genes in pathogenic E.coli

Detection of cnf1 and cnf2 Genes in Clinical Isolates of E.coli

Prevalence of Extended-Spectrum B-Lactamase (ESBL) and Quinolone Resistance (qnr) Genes among Cytotoxic Necrotizing Factor-1-Producing Uropathogenic Escherichia coli in Babylon, Iraq

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