Prevalence and Cross-Immunity of Eimeria Species on Korean Chicken Farms

Lee, Byung H.; Kim, Woo Hyun; Jeong, Junhui; Yoo, Jeongmi; Kwon, Yong-Kuk; Jung, Byeong Yeal; Kwon, Jun-Hun; Lillehoj, Hyun S.; Min, Wongi

doi:10.1292/jvms.09-0517

Cited by 56 publications

(51 citation statements)

References 23 publications

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“…The number of oocysts appears to be a limiting factor for identication through PCR. Lee et al (2010) used 50 oocysts for individual amplifications. Fernandez et al (2003b) found a minimum limit of 50 oocysts for detection of six species using the PCR multiplex, while the limit for E. tenella was found to be 100 oocysts both in individual and multiplex reactions.…”

Section: Dicussionmentioning

confidence: 99%

“…According to Conway et al (1993), only 100 E. acervulina and E. tenella oocysts are needed to cause serious gastrointestinal disorders in birds. Furthermore, molecular diagnosis can accurately reveal the prevalence of Eimeria species, thus contributing to the strategic employment of medicines and vaccines (Sun et al, 2009;Lee et al, 2010).…”

Section: Dicussionmentioning

confidence: 99%

“…Molecular techniques may also contribute to the development of new vaccines and selection of anticoccidial drugs to be used in control programs (Morris and Gasser, 2006;Sun et al, 2009;Lee et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

Fs¹,

Aa²,

Teixeira³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.

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Section: Dicussionmentioning

confidence: 99%

Section: Dicussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

Fs¹,

Aa²,

Teixeira³

et al. 2011

Genet. Mol. Res.

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“…Prevalence and identification of Eimeria species are useful tools for prevention and control of coccidiosis [4]. The prevalence may vary from less than 10 % to more than 90 % in broilers worldwide [5,6,7,4].…”

Section: Introductionmentioning

confidence: 99%

Eimeria Spp.Infection in Some Broiler Farms in Khartoum State, Sudan

Abdelnabi¹

2017

SOJVS

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A cross sectional study was conducted during October 2013 to February 2014 to identify and determine the prevalence of Eimeria spp. among broiler chickens reared in open and closed systems in Khartoum, Khartoum North, and Omdurman, Khartoum State, Sudan. A total of 90 broiler intestines were collected from slaughtered broilers at slaughterhouse or farms and examined by direct smear and standard floatation test to detect Eimeria species. The overall prevalence of Eimeria spp. was 5.5 % of the examined samples. Two Eimeria species were identified viz, Eimeria tenella and Eimeria acervulina. E. tenella was the dominant species with an infection rate of 5.5 % followed by E. acervulina 2.2 %. The prevalence of Eimeria spp. in open and closed systems was 4.4 % and 6.7 %, respectively. The overall prevalence of Eimeria spp. was 10 %, 6.7 % and 0 % in Khartoum, Khartoum North and Omdurman, respectively. No significant associations (P ˃ 0.05) were observed between different locations and management systems employed of the examined chickens. Further epidemiological studies should be conducted to determine the prevalence and identify Eimeria spp. in all states of the Sudan.

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“…Occurrences of coccidiosis in commercial poultry can range from 5 to 70% (DU;HU, 2004;FREITAS et al, 2008;AARTHI et al, 2010;LEE et al, 2010;TOLEDO et al, 2011), and economic losses have been widely reported (DALLOUL; LILLEHOJ, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of the granulometric characteristics of monensin sodium on controlling experimental coccidiosis in broiler chickens

Rambozzi

Renna

Cornale

et al. 2012

Rev. Bras. Parasitol. Vet.

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The aim of the present study was to investigate the efficacy of monensin sodium grain size on controlling coccidiosis in broiler chickens. Three hundred and fifty Cobb 700 chicks were infected experimentally with 8 × 10 4 sporulated oocysts of Eimeria spp. The birds were fed with diets supplemented with powdered (n = 150) or granulated (n = 150) monensin, with particle sizes of ≤100 µm and 450-650 µm, respectively. The remaining chicks (n = 50) were used as an unmedicated control group. The birds' weights and number of oocysts per gram of litter in each group were recorded weekly. A total of 96 birds were randomly selected and culled during the trial. Their intestinal oocyst counts and lesion scores were determined. No significant differences in body weights were found between the groups at the end of the study. The percentages of infected animals, oocyst counts and lesion scores were significantly higher in the control group than in the other two groups; the two treated groups did not show any appreciable differences to each other. Enteritis was observed in 14 birds, all in the group supplemented with granulated monensin. These results suggest that powdered and granulated monensin have comparable efficacy in controlling coccidiosis in broiler chickens. However, treatments with monensin sodium of high grain size may be subject to contraindications relating to enteritis.Keywords: Gallus gallus, coccidiostatic drug, Eimeria, particle size. ResumoO presente estudo teve como objetivo investigar a eficácia da monensina sódica com diferentes granulometrias no controle da coccidiose em frangos de corte. Trezentos e cinquenta pintos Cobb 700 foram infectados experimentalmente, utilizando-se 8 × 10 4 oocistos esporulados de Eimeria spp. As aves foram alimentadas com dietas suplementadas com monensina em pó (n = 150) ou granulada (n = 150), com partículas de dimensão ≤100 µm e de 450-650 µm respetivamente. As aves restantes (n = 50) foram utilizadas como grupo controle não sujeito a tratamento. O peso das aves e o número de oocistos por grama de material de cama de cada grupo foram avaliados semanalmente. Um total de 96 aves foi selecionado aleatoriamente e submetidos à eutanásia durante o período experimental. Nestas aves foram analisados o número de oocistos intestinais e o respectivo escore das lesões. Não foram encontradas diferenças significativas relativas ao peso dos animais dos diferentes grupos no final do período experimental. A proporção de animais infectados, a quantidade de oocistos e o escore das lesões foram significativamente superiores no grupo de controle, quando comparado com os dois grupos tratados, não encontrando diferenças significativas entre estes últimos. Foi observada enterite em 14 aves, pertencentes ao grupo suplementado com monensina granulada. Estes resultados indicam que a eficácia da monensina em pó e granulada é semelhante no controle de coccidiose em frangos de corte. No entanto, o tratamento com monensina sódica de elevada granulometria pode ser objeto de contraindicações associ...

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Prevalence and Cross-Immunity of Eimeria Species on Korean Chicken Farms

Cited by 56 publications

References 23 publications

Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

Eimeria Spp.Infection in Some Broiler Farms in Khartoum State, Sudan

Effect of the granulometric characteristics of monensin sodium on controlling experimental coccidiosis in broiler chickens

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