Prevalence and concordance of high-risk papillomavirus infection in male sexual partners of women diagnosed with high grade cervical lesions

Diez, Elena López; Pérez, Sonia González; Iñarrea, Amparo; Orden, Angel de la; Castro, Máximo; Almuster, Sheila; Tortolero, L.; Rodríguez, M. Samprón; Montero, Ruben; Ojea, A

doi:10.1016/j.eimc.2016.02.014

Cited by 7 publications

(12 citation statements)

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“…This confirmed once again the close association between persistent infection in women and viral genotypes with high oncogenic potential, resulting in an increased risk of neoplastic evolution of the injury [ 20 ]. These results also confirmed that the sexual contact is the main way of transmission of HPV infection and the concordance of at least one viral genotype, in each case at high risk, in the majority of couples would confirm the hypothesis, already advanced in the recent literature [ 21 , 22 ], of an important role of infected male in the transmission and/or maintenance of infection in women with an increased risk of cervical cancer. In this study, for the first time, we applied a protocol of differential extraction from semen achieved by differential lysis of epithelial cells and sperm in the absence and presence of DTT that is used also in forensic biology protocols for forensic STR analysis when DNA from epithelial cells (of victim) and sperm (from perpetrator) in samples from sexual assault have to be isolated separately for obtaining independent genotypes [ 23 ].…”

Section: Discussionsupporting

confidence: 86%

HPV infection in semen: results from a new molecular approach

et al. 2019

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Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseases causing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPV infection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developed a new approach to evaluate virus localisation in the different semen components. We analysed also the specific genotype localisation and viral DNA quantity by qPCR. Results show that HPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and several HPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPV DNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective, suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support the proposed role of HPV in decreased fertility and prompt new possible consequences of the infection in semen.

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Section: Discussionsupporting

confidence: 86%

HPV infection in semen: results from a new molecular approach

et al. 2019

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“…From the 7682 abstracts reviewed, 35 articles were selected and among them, 12 met the inclusion criteria (Table 1) [11–22]. Reasons for the exclusion of 23 articles were that they overlapped with other included studies, they were written in non-English language or they were unrelated to the study subject (see Table 2).…”

Section: Resultsmentioning

confidence: 99%

Prevalence of human papillomavirus and subtype distribution in male partners of women with cervical intraepithelial neoplasia (CIN): a systematic review

et al. 2019

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Background Human Papillomavirus (HPV) infection is estimated to be the most common sexually transmitted infection. The present systematic review summarizes data regarding the prevalence of HPV and the distribution of subtypes in heterosexual male partners of women, who were diagnosed with any grade of cervical intraepithelial neoplasia (CIN). Methods We conducted a systematic review of the literature by Medline and Google Scholar databases using the terms “Human Papillomavirus” or “HPV” plus “men” or “male partners” or “women with CIN”. We included original published English-language articles published from 1/1/2000 until 1/1/2018 that had screened male partners of women with CIN using HPV DNA testing. We excluded studies that they overlapped with other included studies or were unrelated to the study subject. Results We included a total of 12 publications, which reported the prevalence of HPV in free-clinical signs male partners of women with CIN. The largest proportion of the studies were from South America (seven studies), and the rest from Europe. The mean age of participants was 35.18 + − 3.47 years. HPV prevalence ranged from 12.9 to 86%; the total HPV prevalence among the studies was 49.1%, while ten out twelve studies (83.3%) demonstrated prevalence > 20%. Between the studies, the distribution of HPV subtypes varied on the basis of the method used, on the population and on the geographic region. A great variety of subtypes were detected, including 6, 11, 16, 18, 31, 33, 40, 42, 45, 51, 52, 53, 54, 56, 57, 58, 59, 61, 62, 66, 68, 81 and 83. In six studies the HPV 16 was the most frequent, while in two others the HPV 6 and HPV 83. Conclusions Until now, there are not precise screening or surveillance guidelines for the management of partners of women with CIN. This population is frequently colonized by various HPV subtypes and therefore need to be screened in an effort to reduce the infection in both sexes. The screening test could include detection/identification of HPV subtypes by a molecular assay, followed by peniscopy only in the positive cases.

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“…The objective of this study was to assess the risk factors associated with HR-HPV infection in a high-risk male population. Male SPs of women with HGCL have been the focus of previous studies [ 8 , 19 – 21 ]. In our population, the prevalence of HR-HPV infection was high (i.e., almost half of the studied cases); this result is in agreement with data reported in similar studies (30%-68%) [ 8 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…Male SPs of women with HGCL have been the focus of previous studies [ 8 , 19 – 21 ]. In our population, the prevalence of HR-HPV infection was high (i.e., almost half of the studied cases); this result is in agreement with data reported in similar studies (30%-68%) [ 8 , 22 ]. Polymerase chain reaction (PCR) is well established as the most sensitive detection method considering that there is no licensed test for HPV detection in men [ 20 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

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Lifestyle factors and oncogenic papillomavirus infection in a high-risk male population

López-Diez

Pérez²,

Carballo

et al. 2017

PLoS ONE

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BackgroundHigh risk human papillomavirus (HR-HPV) infection in males is a health issue with implications for HPV-related lesions in their partners. The identification of risk factors for male infection may improve our understanding of HR-HPV transmission and prevention. The aim of this study was to evaluate the relationships between lifestyle, genital warts and HR-HPV infection. The study was focused on men with an increased risk of HR-HPV infection: male sexual partners of women diagnosed with high-grade squamous intraepithelial cervical lesions.MethodsMen were enrolled and prospectively recruited within the first six months after diagnosis of cervical lesions in their female partners (n = 175, 2013–2016). Epidemiological and sexual behaviour data were obtained. The presence of genital warts was established by visual inspection. Detection and genotyping of HR-HPV infection in genital samples were performed with a Linear Array HPV Genotyping Test. All HR-HPV positive men were offered a follow-up exam at 12 months. SPSS version 19 was used for statistical analysis.Results and discussionThe prevalence of HR-HPV infection in men was 45.1% (79/175). Genital warts were observed in 10.3% (18/175) of the subjects. Detection of genital warts (OR 3.5, p = 0.015), smoking habits (OR 2.3, p = 0.006) and sexual debut before 16 years old (OR 2, p = 0.035) were associated with an increased risk for HR-HPV infection (univariate analysis). This association was also observed for genital warts and smoking status in a multivariate analysis. The same genotype was found after one year in 71.4% (20/28) of subjects.ConclusionsThe presence of genital warts and smoking habits seem to be associated with a higher risk of HR-HPV infection in males. Earlier sexual debut may increase this risk. Extensive knowledge of the natural history of HR-HPV infection in males is an absolute requirement for the design and implementation of prevention strategies for the general population as well as for specific populations such as couples after treatment for high-grade cervical lesions.

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Prevalence and concordance of high-risk papillomavirus infection in male sexual partners of women diagnosed with high grade cervical lesions

Cited by 7 publications

References 31 publications

HPV infection in semen: results from a new molecular approach

HPV infection in semen: results from a new molecular approach

Prevalence of human papillomavirus and subtype distribution in male partners of women with cervical intraepithelial neoplasia (CIN): a systematic review

Lifestyle factors and oncogenic papillomavirus infection in a high-risk male population

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