Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

Briand, Valérie; Cottrell, Gilles; Ndam, Nicaise Tuikue; Martiáñez–Vendrell, Xavier; Vianou, Bertin; Mama, Atika; Kouwaye, Bienvenue; Houzé, Sandrine; Bailly, Justine; Gbaguidi, Erasme; Sossou, Darius; Massougbodji, Achille; Accrombessi, Manfred; Mayor, Alfredo; Ding, Xavier C.; Fiévet, Nadine

doi:10.1186/s12936-020-03261-1

Cited by 22 publications

(25 citation statements)

References 22 publications

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“…Therefore, this suggests that uRDTs do not provide added benefits to the detection of infections among febrile individuals in the community when compared to regular RDTs, a finding that has been similarly observed among febrile outpatients in Tanzania [ 27 ]. Similarly, studies conducted among pregnant women observed a higher performance of uRDTs among afebrile women compared to febrile ones [ 28 , 30 ]. These studies were conducted under laboratory conditions using retrospectively collected samples [ 28 – 30 ].…”

Section: Discussionmentioning

confidence: 91%

“…Similarly, studies conducted among pregnant women observed a higher performance of uRDTs among afebrile women compared to febrile ones [28,30]. These studies were conducted under laboratory conditions using retrospectively collected samples [28][29][30]. However, there is currently no evidence of the performance of uRDTs among pregnant women when the tests are directly conducted at point of care.…”

Section: Discussionmentioning

confidence: 99%

“…Studies evaluating the potential role of uRDTs in case management found no differences in the performance of uRDTs and cRDTs for clinical malaria diagnosis among non-pregnant patients [27]. Finally, a similar or higher performance of uRDTs compared to cRDTs has been reported in pregnant women when tested using retrospectively collected peripheral or placental blood samples [28][29][30].…”

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confidence: 92%

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Field performance of ultrasensitive and conventional malaria rapid diagnostic tests in southern Mozambique

Galatas

Mayor

Gupta

et al. 2020

Malar J

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Background An ultrasensitive malaria rapid diagnostic test (RDT) was recently developed for the improved detection of low-density Plasmodium falciparum infections. This study aimed to compare the diagnostic performance of the PfHRP2-based Abbott Malaria Ag P. falciparum ultrasensitive RDT (uRDT) to that of the conventional SD-Bioline Malaria Ag P. falciparum RDT (cRDT) when performed under field conditions. Methods Finger-prick blood samples were collected from adults and children in two cross-sectional surveys in May of 2017 in southern Mozambique. Using real-time quantitative PCR (RT-qPCR) as the reference method, the age-specific diagnostic performance indicators of the cRDT and uRDT were compared. The presence of histidine-rich protein 2 (HRP2) and Plasmodium lactate dehydrogenase (pLDH) antigens was evaluated in a subset from dried blood spots by a quantitative antigen assay. pfhrp2 and pfhrp3 gene deletions were assessed in samples positive by RT-qPCR and negative by both RDTs. Results Among the 4,396 participants with complete test results, the sensitivity of uRDTs (68.2; 95% CI 60.8 to 74.9) was marginally better than that of cRDTs (61.5; 95% CI 53.9 to 68.6) (p-value = 0.004), while the specificities were similar (uRDT: 99.0 [95% CI 98.6 to 99.2], cRDT: 99.2 [95% CI 98.9 to 99.4], p-value = 0.02). While the performance of both RDTs was lowest in ≥ 15-year-olds, driven by the higher prevalence of low parasite density infections in this group, the sensitivity of uRDTs was significantly higher in this age group (54.9, 95% CI 40.3 to 68.9) compared to the sensitivity of cRDTs (39.2, 95% CI 25.8 to 53.9) (p-value = 0.008). Both RDTs detected P. falciparum infections at similar geometric mean parasite densities (112.9 parasites/μL for uRDTs and 145.5 parasites/μL for cRDTs). The presence of HRP2 antigen was similar among false positive (FP) samples of both tests (80.5% among uRDT-FPs and 84.4% among cRDT-FPs). Only one false negative sample was detected with a partial pfhrp2 deletion. Conclusion This study showed that the uRDTs developed by Abbott do not substantially outperform SD-Bioline Pf malaria RDTs in the community and are still not comparable to molecular methods to detect P. falciparum infections in this study setting.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 92%

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Field performance of ultrasensitive and conventional malaria rapid diagnostic tests in southern Mozambique

Galatas

Mayor

Gupta

et al. 2020

Malar J

Self Cite

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“…It was hoped that with this increased sensitivity, uRDTs would be able to detect enough asymptomatic infections to extrapolate total infection rates based on statistical modeling. The Alere Ultrasensitive Ag Pf RDT is now commercially available and has been tested in a number of field trials in different transmission settings (35)(36)(37)(38)(39)(40)(41)(42)(43) (Table 3).…”

Section: Ultrasensitive Rapid Diagnostic Tests (Urdts)mentioning

confidence: 99%

Ultrasensitive Diagnostics for Low-Density Asymptomatic Plasmodium falciparum Infections in Low-Transmission Settings

Zainabadi

2021

J Clin Microbiol

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The emergence of multidrug resistant Plasmodium falciparum malaria in Southeast Asia has accelerated regional malaria elimination efforts. Most malaria in this and other low transmission settings exists in asymptomatic individuals, which conventional diagnostic tests lack the sensitivity to detect. This has led to the development of new ultrasensitive diagnostics that are capable of detecting these low parasitemic infections. This review summarizes the current status of ultrasensitive technologies, including PCR and LAMP-based methods, as well as a newly developed ultrasensitive rapid diagnostic test (uRDT). The sensitivity, specificity, and field performance of these platforms will be examined, as well as their suitability for use in resource limited settings to aid in malaria elimination efforts. uRDTs, with their improved sensitivity, are now able to detect approximately half of asymptomatic infections, providing a useful point of contact tool for malaria surveillance. The increased sensitivity and high-throughput nature of PCR-based tests make them ideal for screening large populations in places where laboratory capacity exists, and the recent commercialization of malaria LAMP kits should facilitate their adoption as a public health tool in places where such infrastructure is lacking. Finally, recent advances with dried blood spots may enable the utilization of the extensive laboratory infrastructure of higher income countries to assist with molecular surveillance in support of malaria elimination. If malaria is to be eliminated in SEA and other low endemic regions, then ultrasensitive diagnostics may play a key role in identifying and clearing the vast asymptomatic pool of infections that are common to these regions.

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“…Following publication of the original article [ 1 ], the authors flagged that there is an error present in Table 5.…”

Section: Correction To: Malar J (2020) 19:188101186/s12936-mentioning

confidence: 99%

Correction to: Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

Briand

Cottrell

Ndam

et al. 2020

Malar J

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Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

Cited by 22 publications

References 22 publications

Field performance of ultrasensitive and conventional malaria rapid diagnostic tests in southern Mozambique

Field performance of ultrasensitive and conventional malaria rapid diagnostic tests in southern Mozambique

Ultrasensitive Diagnostics for Low-Density Asymptomatic Plasmodium falciparum Infections in Low-Transmission Settings

Correction to: Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

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