Prevalence and Characterization of Staphylococcus aureus Cultured From Raw Milk Taken From Dairy Cows With Mastitis in Beijing, China

Wang, Wei; Lin, Xiaohui; Jiang, Tao; Peng, Zong–Gen; Xu, Jin; Yi, Long; Li, Fengqin; Fanning, Séamus; Baloch, Zulqarnain

doi:10.3389/fmicb.2018.01123

Cited by 112 publications

(154 citation statements)

References 70 publications

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“…In consistent with the previous reports, all ST398 isolates in this study expressed at least one fibronectin-binding protein, while some of the isolates expressed both FnBPA and FnBPB. A previous study showed that five out of the six ST398 strains isolated from raw milk of dairy cows with mastitis were positive of both fnbpA and fnbpB , while the other one isolate only carried fnbpA [23]. However, an MRSA ST398 strain from urinary tract infection in a child only carried fnbpB [24].…”

Section: Discussionmentioning

confidence: 99%

Characterisation ofStaphylococcus aureusisolated from rabbits in Fujian, China

et al. 2019

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Staphylococcus aureus has been recognised as one of the important zoonotic pathogens. However, knowledge about the epidemiology and genetic characteristics of S. aureus in rabbits was limited. The aim of this study was to determine the characteristics of 281 S. aureus isolated from dead rabbits of nine rabbit farms in Fujian Province, China. All the isolates were characterised by multi-locus sequencing typing, detection of virulence factors and antimicrobial susceptibility test. The results showed that the 281 isolates were grouped into two sequence types, ST121 (13.52%, 38/281) and ST398 (86.48%, 243/281). Surprisingly, the ST121 strains were only recovered from the lung samples from one of the nine rabbit farms studied. In the 281 isolates, the virulence genes of nuc, hla, hlb, clfA, clfB and fnbpA were positive, whereas the sea, seb, tsst, eta and etb genes were negative. Notably, the 38 ST121 isolates carried the pvl gene. All the 281 isolates were methicillin-susceptible S. aureus, and the isolates were susceptible to most of the used antibiotics, except for streptomycin, kanamycin, azithromycin and penicillin, and the resistance rates of which were 23.84%, 19.57%, 16.01% and 11.03%, respectively. This study first described the epidemiology and characteristics of S. aureus in rabbits in Fujian Province, which will help in tracking the evolution of epidemic strains and preventing the rabbit–human transmission events.

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Section: Discussionmentioning

confidence: 99%

Characterisation ofStaphylococcus aureusisolated from rabbits in Fujian, China

et al. 2019

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“…Similarly, multiresistant strains of CC97 were isolated from human patients in Saudi Arabia (Monecke et al, 2012) and in bovine in Spain (Gómez-Sanz et al, 2010). In contrast, non-multiresistant isolates of CC97 were obtained from dairy milk in China (Wang et al, 2018). We observed differences between the antibiotic susceptibility patterns and the carriage of antibiotic resistance genes in CC97 isolates obtained in this study.…”

Section: Discussionmentioning

confidence: 61%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Patients Admitted to Kuwait Hospitals in 2016–2017

et al. 2020

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has been reported to colonize and cause infections in animals as well as in humans. LA-MRSA isolates have only recently been identified in patients admitted to Kuwait hospitals. This study was conducted to characterize LA-MRSA isolates obtained from patients admitted to Kuwait hospitals. A total of 202 (7.1%) of 2,823 MRSA isolates obtained from clinical samples in 2016 and 2017 in 11 public Kuwait hospitals were assigned to lineages previously known to be associated with livestock. They were characterized using antibiogram, spa typing, and DNA microarray for the assignment of clonal complexes (CCs) and detection of antibiotic resistance and virulence determinants. Identification as putative LA-MRSA clones was based on the molecular definition inferred from DNA microarray. The LA-MRSA isolates consisted of CC96 (N = 31), CC97 (N = 169), and CC398 (N = 2). Isolates belonging to CC96 and CC398 were resistant to erythromycin and clindamycin mediated by erm(A) and erm(C). CC97 isolates were multiresistant to gentamicin, kanamycin, erythromycin, clindamycin, tetracycline, chloramphenicol, fusidic acid, trimethoprim, and ciprofloxacin and harbored aacA-aphD, erm(A), erm(C), msr(A), tet(K), cat, fusC, and dfrS1. In total, 35 spa types were identified among the isolates. CC398 isolates consisted of t899 and t034. Ten spa types were identified among CC96 with t11822 (N = 13) as the most prevalent. CC97 consisted of 26 spa types with most belonging to t267 (N = 73) followed by t359 (N = 39). CC398 was composed of CC398-MRSA-IV and CC398-MRSA-V (PVL +). CC96 belonged to CC96-MRSA-IV and CC96-MRSA-IV (PVL +) Central Asian caMRSA/WA MRSA-119. CC97 consisted of six strains including CC97-MRSA-V (fusC +), CC97-MRSA-IV WA MRSA-54/63, CC97-MRSA-V, CC97-MRSA-(V+fus), CC97-MRSA-(mec VI+fus), and CC97-MRSA (mecV/V T +fus+ccrAB2). Whereas CC96 and CC97 isolates were identified in 2016 and 2017, CC398 isolates were detected only in 2016. This study identified four LA-MRSA clones among MRSA isolated from patients in Kuwait hospitals in 2016-2017 with CC97-MRSA-V (fusC +) as the dominant clone. The presence of LA-MRSA with different genetic backgrounds suggests its independent acquisition from different sources.

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“…Similar results have been reported previously from Malaysia, although the prevalence among these studies varies (Othman and Bahaman, 2005;Murugaiyah et al, 2014;Ahamed et al, 2016). High prevalence trend of S. aureus in association of subclinical mastitis has been reported elsewhere in France, China, and Ethiopia (Birhanu et al, 2017;Poutrel et al, 2018;Wang et al, 2018). Is one of the main pathogens for contagious mastitis, and it is believed to be transmitted during milking (Anderson et al, 2012).…”

Section: Discussionmentioning

confidence: 92%

Prevalence and Antimicrobial Resistance Profiles of Bacterial Isolates Associated with Intramammary Infections in Malaysian Dairy Herds

Zunita

Jesse

Ramanoon

et al. 2020

Preprint

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Intramammary infections are costly endemic disease in dairy cows and it highly affects both the quality and quantity of milk production in addition to the animal well-being. It a major cause of considerable economic losses to the dairy farmers. This study was aimed to determine the prevalence of mastitis pathogens and antimicrobial resistance pattern of selected most prevalent pathogens. A total of 1945 quarter samples from 517 cows across 33 dairy herds were used. Isolation and identification of mastitis pathogens was carried out based on standard bacteriological procedures. Antimicrobial resistance profile was conducted by disk diffusion technique. The overall culture prevalence was 67.6% (n=503/744, CI= 64.1-70.9). The respective states’ culture prevalence were 66.4% (n=71/107, CI= 56.5-75.0), 60.3% (184/305, CI= 54.6-65.8), 58% (n=94/162, CI= 50-65.6), 100% (n=75/75, CI= 93.9-100), and 83.3% (n=79/95, CI= 73.8-89.8) for Pahang, Perak, Selangor, Negeri Sembilan and Johor respectively. The most prevalent isolates identified were non-aureus staphylococci (NAS) 39.5%, S. aureus 13.1%, K. pneumoniae 6.5%, S. agalactiae 4.8%, and S. uberis 4.3%. Resistance profile of K. pneumoniae isolates against ampicillin and Penicillin G were 70.4% and 88.9% respectively while that of Actinobacter spp against chloramphenicol and streptomycin were 71.4% and 35.7% respectively. For S. uberis isolates, 66.7% and 73.3% have shown resistance to tetracycline and streptomycin respectively. This study provides knowledge of the epidemiology of bacterial isolates associated with subclinical mastitis in Malaysia and their resistance profile. The high prevalence of these pathogens in milk and multidrug resistance observed should not be over looked.

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Prevalence and Characterization of Staphylococcus aureus Cultured From Raw Milk Taken From Dairy Cows With Mastitis in Beijing, China

Cited by 112 publications

References 70 publications

Characterisation ofStaphylococcus aureusisolated from rabbits in Fujian, China

Characterisation ofStaphylococcus aureusisolated from rabbits in Fujian, China

Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Patients Admitted to Kuwait Hospitals in 2016–2017

Prevalence and Antimicrobial Resistance Profiles of Bacterial Isolates Associated with Intramammary Infections in Malaysian Dairy Herds

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