Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius in pets from South China

Feng, Yue; Tian, Wei; Lin, Dachuan; Luo, Qianyi; Zhou, Yingze; Yang, Tong; Deng, Yuting; Liu, Yahong; Liu, Jianhua

doi:10.1016/j.vetmic.2012.06.015

Cited by 67 publications

(57 citation statements)

References 29 publications

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“…Unfortunately, there are several recent reports of MRSP isolates that exhibited high rates of prevalence in FarEastern parts of the world. In China, 69 of 144 (47.9%) S. pseudintermedius were identified as MRSP [25] . In Japan, 27 MRSP characterized by the growth on chromID MRSA agar and confirmed by the presence of mecA gene, in 200 cats and dogs, most of which were isolated from dogs (n: 25) [26] .…”

Section: Discussionmentioning

confidence: 99%

Methicillin Resistance in Staphylococcus pseudintermedius Isolated from Shelter Dogs in Turkey

Sareyyüpoğlu¹,

Müştak²,

Cantekin³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryStaphylococcus pseudintermedius is a recently described species of staphylococci that is frequently isolated from dogs. The increase in antimicrobial resistance in staphylococci and transfer of S. pseudintermedius from infected pets to humans threaten the public health worldwide. In this study, it was aimed to determine the prevalence of S. pseudintermedius from skin infections of dogs and to determine the prevalence of methicillin-resistance in S. pseudintermedius isolates by phenotypic and genotypic methods. A total of 41 staphylococci were isolated from 53 dogs. Thirty three (80.4%) staphylococci were identified as S. pseudintermedius by PCR-RFLP analysis of pta gene. Methicillin resistance was also identified in 11 (33.3%) of these isolates by inoculation on chromogenic chromID MRSA agar, oxacillin disc diffusion method and the determination of mecA gene by PCR. This is the sole report in Turkey that described the situation of methicillin resistant S. pseudintermedius. Keywords: Dog, Staphylococcus pseudintermedius, Methicillin resistance Türkiye'deki Barınak Köpeklerinden İzole Edilen Staphylococcus pseudintermedius'larda Metisilin Direnci ÖzetStaphylococcus pseudintermedius, stafilokoklar içerisinde yeni tanımlanmış bir tür olup köpeklerden sıklıkla izole edilmektedir. Stafilokoklarda artan antimikrobiyal direnç ve S. pseudintermedius'un enfekte petlerden insanlara geçmesi, halk sağlığını tehdit etmektedir. Bu çalışmada, köpek deri enfeksiyonlarından izole edilen S. pseudintermedius'ların ve metisilin direncinin prevalansının fenotipik ve genotipik metotlar ile belirlenmesi amaçlanmıştır. Toplam 53 köpekten 41 stafilokok izole edildi. Stafilokok izolatlarının 33'ü (%80.4) pta geninin PCR-RFLP ile analiziyle S. pseudintermedius olarak belirlendi. Metisilin direnci kromogenik chromID MRSA agar, oxacillin disk difüzyon metodu ve mecA geninin belirlendiği PCR yöntemi ile bu izolatların 11'inde (%33.3) tespit edildi. Bu çalışma, Türkiye'de metisilin dirençli S. pseudintermedius'ların durumunu gösteren ilk bilimsel rapordur.

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Section: Discussionmentioning

confidence: 99%

Methicillin Resistance in Staphylococcus pseudintermedius Isolated from Shelter Dogs in Turkey

Sareyyüpoğlu¹,

Müştak²,

Cantekin³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…S. pseudintermedius has been documented to cause invasive infections, including brain abscesses, endocarditis, and bacteremia, in humans (3). Methicillin resistance among S. pseudintermedius isolated from dogs is increasing (4), with rates of up to 47% in some regions of the world (5). This resistance is predominantly due to the dissemination of the ST71 clonal lineage in Europe and the ST68 clonal lineage in North America (4).…”

Section: T He Staphylococcus Intermedius Group (Sig) Is Comprised Of mentioning

confidence: 99%

In Vitro Antimicrobial Susceptibility of Staphylococcus pseudintermedius Isolates of Human and Animal Origin

Humphries

Westblade

et al. 2016

J Clin Microbiol

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h MIC results for 115 Staphylococcus intermedius group isolates are presented. Of these, 33% were methicillin resistant, among which 51.4% were susceptible to doxycycline, 29.7% to clindamycin, and 21.6% to trimethoprim-sulfamethoxazole. All of the isolates were susceptible to ceftaroline, daptomycin, linezolid, nitrofurantoin, quinupristin-dalfopristin, rifampin, tigecycline, and vancomycin. Of all the isolates, 82.6%, 67.8%, and 23.5% were susceptible to ciprofloxacin, erythromycin, and penicillin, respectively. No isolates harbored mupA or qacA/B genes, which suggested a lack of resistance to mupirocin or chlorhexidine. T he Staphylococcus intermedius group (SIG) is comprised ofStaphylococcus intermedius, Staphylococcus pseudintermedius, and Staphylococcus delphini. These Gram-positive cocci (except for S. intermedius) are positive for tube coagulase and negative for slide coagulase and may be misidentified as Staphylococcus aureus by clinical laboratories that test human specimens (1). A colonizer of the nares and anal mucosa of cats and dogs, S. pseudintermedius is increasingly being recognized in human diagnostic specimens (2). This may be due in part to improved diagnostic technologies, such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), now being used in many clinical laboratories. S. pseudintermedius has been documented to cause invasive infections, including brain abscesses, endocarditis, and bacteremia, in humans (3). Methicillin resistance among S. pseudintermedius isolated from dogs is increasing (4), with rates of up to 47% in some regions of the world (5). This resistance is predominantly due to the dissemination of the ST71 clonal lineage in Europe and the ST68 clonal lineage in North America (4). Methicillin-resistant isolates often display resistance to other classes of antimicrobials used in veterinary medicine, including aminoglycosides, fluoroquinolones, lincosamides, macrolides, and tetracyclines, and to chloramphenicol and trimethoprim-sulfamethoxazole (SXT) (6). However, limited susceptibility data are available for S. pseudintermedius with antimicrobials used for humans. We recently conducted a study to evaluate oxacillin and cefoxitin disk and MIC results as predictors of methicillin resistance (encoded by mecA) in a collection of 115 SIG isolates from human and veterinary specimens associated with clinical infections. This study documented that cefoxitin testing, which is recommended by the Clinical and Laboratories Standards Institute (CLSI) to predict methicillin resistance for other species of staphylococci, is a poor predictor of mecA in SIG, whereas both oxacillin disk and MIC tests accurately detect mecA-mediated oxacillin resistance in these isolates (7). As a result of our study, CLSI published S. pseudintermedius-specific oxacillin breakpoints in the 26th edition of the M100S standard (8). In the present study, we document the results of antimicrobial susceptibility testing (AST) for this collection of 115 SIG isolates, includin...

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“…Methicillin-resistant S. pseudintermedius (MRSP) has recently emerged in different parts of the world (4)(5)(6). MRSP is resistant to all beta-lactam antimicrobials and is generally resistant to three or more antimicrobial classes (multidrug resistant [MDR]), which poses a challenge to therapy in veterinary medicine.…”

mentioning

confidence: 99%

“…Multilocus sequence typing (MLST) has shown that sequence type 71 (ST71) is a dominant clonal lineage that has spread successfully in Europe and Asia, whereas ST68 has spread mainly in North America (15). A limited number of MRSP STs have been identified that are reflected in the dissemination of a few dominant clonal lineages (5,(15)(16)(17). Isolates belonging to ST71 and ST68 often contain genes that confer resistance to multiple antimicrobials in addition to the mecA gene on the staphylococcal cassette chromosome mec element (SCCmec), which encodes methicillin resistance (15,18).…”

mentioning

confidence: 99%

Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

et al. 2016

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Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius in pets from South China

Cited by 67 publications

References 29 publications

Methicillin Resistance in Staphylococcus pseudintermedius Isolated from Shelter Dogs in Turkey

Methicillin Resistance in Staphylococcus pseudintermedius Isolated from Shelter Dogs in Turkey

In Vitro Antimicrobial Susceptibility of Staphylococcus pseudintermedius Isolates of Human and Animal Origin

Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

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