Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina

Gardella, Noella; Murzicato, Sofía; Gregorio, Sabrina Di; Cuirolo, Arabela; Desse, Javier; Crudo, Favio; Gutkind, Gabriel; Mollerach, Marta

doi:10.1016/j.meegid.2011.03.019

Cited by 33 publications

(27 citation statements)

References 29 publications

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“…In previous studies in Latin America including children from DCCs of similar age to the ones in this study, the frequency of MRSA colonization varies, for example in Mexico, 0.93% [31], Brazil, 1.2% [6], Cuba, 2.2% [32] and Argentina 4.4% [33]. In the present study, MRSA colonization in DCCs children (3.5%), is similar to the frequencies detected in these countries, but lower than previously reported in other Colombian cities, e.g.…”

Section: Resultsmentioning

confidence: 56%

“…Notoriously, CC121 is uncommon among resistant isolates [45]. Finally, MRSA genotypes found in both populations differed from those detected at highest frequencies in colonized children of countries such as Brazil (CC8-ST239-SCC mec IVc-III) [6] and Argentina (CC5-ST5-SCC mec IV) [33]. Data that further evidences changes in the epidemiology of colonizing MRSA among different countries.…”

Section: Resultsmentioning

confidence: 75%

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Differences in Epidemiological and Molecular Characteristics of Nasal Colonization with Staphylococcus aureus (MSSA-MRSA) in Children from a University Hospital and Day Care Centers

et al. 2014

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BackgroundClinical significance of Staphylococcus aureus colonization has been demonstrated in hospital settings; however, studies in the community have shown contrasting results regarding the relevance of colonization in infection by community-associated MRSA (CA-MRSA). In Colombia there are few studies on S. aureus colonization. The aim of this study was to determine the molecular and epidemiological characteristics of nasal colonization by S. aureus (MSSA-MRSA) in children from a university hospital and day care centers (DCCs) of Medellin, Colombia.MethodsAn observational cross-sectional study was conducted in 400 children (200 in each setting), aged 0 months to 5 years, during 2011. Samples were collected from each nostril and epidemiological information was obtained from the parents. Genotypic analysis included spa typing, PFGE, MLST, SCCmec typing, detection of genes for virulence factors and agr groups.ResultsFrequency of S. aureus colonization was 39.8% (n = 159) (hospital 44.5% and DCCs 35.0%) and by MRSA, 5.3% (n = 21) (hospital 7.0% and DCCs 3.5%). Most S. aureus colonized children were older than two years (p = 0.005), the majority of them boys (59.1%), shared a bedroom with a large number of people (p = 0.028), with history of β-Lactamase inhibitors usage (p = 0.020). MSSA strains presented the greatest genotypic diversity with 15 clonal complexes (CC). MRSA isolates presented 6 CC, most of them (47.6%) belonged to CC8-SCCmec IVc and were genetically related to previously reported infectious MRSA strains.ConclusionDifferences in epidemiological and molecular characteristics between populations may be useful for the understanding of S. aureus nasal colonization dynamics and for the design of strategies to prevent S. aureus infection and dissemination. The finding of colonizing MRSA with similar molecular characteristics of those causing infection demonstrates the dissemination capacity of S. aureus and the risk of infection among the child population.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 75%

Differences in Epidemiological and Molecular Characteristics of Nasal Colonization with Staphylococcus aureus (MSSA-MRSA) in Children from a University Hospital and Day Care Centers

et al. 2014

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“…In Latin-American countries, specially Argentina and Brazil, other authors have reported the presence of PVLproducer and non-producer MRSA strains carrying SCCmec IV, which cause both community and health-care associated infections (mainly severe SSTI, bacteraemia, osteomyelitis and pneumonia or chronic lung infection) and colonise healthy adults and children (Trindade et al 2005, de Miranda et al 2007, Rozenbaum et al 2009, Scribel et al 2009, Gardella et al 2011, Mimica et al 2011. Nevertheless, there is little information available about the virulence factors present in the isolates and most studies do not include SCCmec typing or any molecular characterisation of the strains.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellín, Colombia

Jiménez

Ocampo

Vanegas

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…For example in the US the initially reported CA-MRSA clone USA 400 was subsequently replaced by the USA 300 which became the most common cause of SSSI [1], [8]. In Argentina previous studies have identified ST5, SCCmec IV, spa type 311 as the predominant CA-MRSA clone causing infections [6] and colonizing children [9]. Microbiologically based studies have suggested the same clonal predominance of CA-MRSA among Argentinean adults [10].…”

Section: Introductionmentioning

confidence: 99%

Prospective Multicenter Study of Community-Associated Skin and Skin Structure Infections due to Methicillin-Resistant Staphylococcus aureus in Buenos Aires, Argentina

Furst¹,

Vedia

Fernandez

et al. 2013

PLoS ONE

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BackgroundCommunity-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is now the most common cause of skin and skin structure infections (SSSI) in several world regions. In Argentina prospective, multicenter clinical studies have only been conducted in pediatric populations.ObjectivePrimary: describe the prevalence, clinical and demographic characteristics of adult patients with community acquired SSSI due to MRSA; secondary: molecular evaluation of CA-MRSA strains. Patients with MRSA were compared to those without MRSA.Materials and MethodsProspective, observational, multicenter, epidemiologic study, with molecular analysis, conducted at 19 sites in Argentina (18 in Buenos Aires) between March 2010 and October 2011. Patients were included if they were ≥14 years, were diagnosed with SSSI, a culture was obtained, and there had no significant healthcare contact identified. A logistic regression model was used to identify factors associated with CA-MRSA. Pulse field types, SCCmec, and PVL status were also determined.ResultsA total of 311 patients were included. CA-MRSA was isolated in 70% (218/311) of patients. Clinical variables independently associated with CA-MRSA were: presence of purulent lesion (OR 3.29; 95%CI 1.67, 6.49) and age <50 years (OR 2.39; 95%CI 1.22, 4.70). The vast majority of CA-MRSA strains causing SSSI carried PVL genes (95%) and were SCCmec type IV. The sequence type CA-MRSA ST30 spa t019 was the predominant clone.ConclusionsCA-MRSA is now the most common cause of SSSI in our adult patients without healthcare contact. ST30, SCCmec IV, PVL+, spa t019 is the predominant clone in Buenos Aires, Argentina.

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Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina

Cited by 33 publications

References 29 publications

Differences in Epidemiological and Molecular Characteristics of Nasal Colonization with Staphylococcus aureus (MSSA-MRSA) in Children from a University Hospital and Day Care Centers

Differences in Epidemiological and Molecular Characteristics of Nasal Colonization with Staphylococcus aureus (MSSA-MRSA) in Children from a University Hospital and Day Care Centers

Characterisation of virulence genes in methicillin susceptible and resistant Staphylococcus aureus isolates from a paediatric population in a university hospital of Medellín, Colombia

Prospective Multicenter Study of Community-Associated Skin and Skin Structure Infections due to Methicillin-Resistant Staphylococcus aureus in Buenos Aires, Argentina

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