Prevalence and characterization of integrons from bacteria isolated from a slaughterhouse wastewater treatment plant

Moura, Alexandra; Henriques, Isabel; Ribeiro, Rogério Pinto; Correia, António

doi:10.1093/jac/dkm340

Cited by 143 publications

(112 citation statements)

References 28 publications

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“…Huys et al [25] and Rhodes et al [26] found more than 85% bacteria of the genus Aeromonas to be oxytetracycline-resistant. In this study, relatively large populations of Enterobacteriaceae microorganisms were reported in both, TET R and OTC R isolates, and similar observations were made by Guillaume et al [27] and Moura et al [28]. A comparison of MIC values and multiple drug resistance of the least resistant TET R isolates in samples of untreated and treated wastewater indicates a drop in resistance to tetracycline, oxytetracycline and a decrease in multiple drug resistance as a result of sewage treatment.…”

Section: Discussionsupporting

confidence: 88%

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

et al. 2017

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Abstract. The objective of this study was to monitor changes (amplification or attenuation) in antibiotic resistance during wastewater treatment based on the ecology of tetracycline-resistant bacteria. The untreated and treated wastewater were collected in four seasons. Number of tetracycline-(TET R ) and oxytetracycline-resistant (OTC R ) bacteria, their qualitative composition, minimum inhibitory concentrations (MICs), sensitivity to other antibiotics, and the presence of tet(A, B, C, D, E) resistance genes were determined. TET R and OTC R counts in untreated wastewater were 100 to 1000 higher than in treated effluent. OTC R bacterial counts were higher than TET R populations in both untreated and treated wastewater. TET R isolates were not dominated by a single bacterial genus or species, whereas Aeromonas hydrophila and Aeromonas sobria were the most common in OTC R isolates. The treatment process attenuated the drug resistance of TET R bacteria and amplified the resistance of OTC R bacteria. In both microbial groups, the frequency of tet(A) gene increased in effluent in comparison with untreated wastewater. Our results also indicate that treated wastewater is a reservoir of multiple drug-resistant bacteria as well as resistance determinants which may pose a health hazard for humans and animals when released to the natural environment.

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Section: Discussionsupporting

confidence: 88%

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

et al. 2017

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“…One isolate, obtained from the mouth of the VR, harbored both classes of integrons, with gene cassette arrays: dfrA1-aadA1 (class 1 integron) and estX-sat2 (class 2 integron). Similar patterns were detected in Aeromonas and Enterobacteriaceae strains isolated from slaughterhouse WWTP (Moura et al 2007), in E. coli of human and animal …”

Section: Class 1 and 2 Integrons In Antimicrobial-resistant E Colisupporting

confidence: 74%

Inadequately Treated Wastewater as a Source of Human Enteric Viruses in the Environment

2015

Wastewater and Public Health

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In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant E. coli were the most frequent, followed by amoxicillin/clavulanate (up to 32 %), trimethoprim/sulfamethoxazole (up to 20 %), and fluoroquinolone (up to 15 %)-resistant isolates. Presence of class 1 and 2 integrons was detected among tested E. coli isolates with rate of 32.06 % (n=84) and 3.05 % (n=8), respectively. The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype. Variable regions were detected in 48 class 1 and 5 class 2 integron-positive isolates. Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays. These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

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“…Overall, among 51 isolates, the overall mean transfer frequencies were Other investigations have demonstrated a transfer frequency at 10 ¡4 to 10 ¡2 transconjugants per recipient CFU of integron-harboring multi-drug-resistant Enterobacteriaceae isolated from clinical settings. 19 Different results, however, were observed by Moura et al 20 in which the frequency was one to 3 orders of magnitude lower at 3.79 £ 10 ¡5 and 5.46 £ 10…”

Section: Resultsmentioning

confidence: 81%

“…Single colonies were inoculated into 10 mL of selective LB broth (Mobio, Carlsbad, California) containing antibiotic(s) similar to LB agar plates which were incubated overnight at 37 C with shaking at 150 rpm. Conjugation assays were performed using a modification of the protocol in Moura et al 20 Donor and recipient cells were incubated separately overnight at 37 C with shaking at 165 rpm in liquid culture containing 5 mL LB broth. Donor and recipient cells were spread and enumerated using plate count assay on Mueller-Hinton (MH) agar before both cells were mixed in ratio of 1:1 and precipitated by centrifugation at room temperature for 5 min at 6700 g. Supernatants were removed and replaced by 1 mL fresh LB followed by incubation at 28 C for 24 h without shaking.…”

Section: Donormentioning

confidence: 99%

Conjugative transmission of antibiotic-resistance from stream waterEscherichia colias related to number of sulfamethoxazole but not class 1 and 2 integrase genes

Suhartono

Savin

2016

Mobile Genetic Elements

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A conjugation assay was used to determine the effects of phenotypic resistance to one to up to 5 antibiotics, sampling site of origin, presence or absence of class 1 and/or class 2 integrase (intI) genes (intI1 and intI2), and the number of sulfamethoxazole resistance (sul) and trimethoprim resistance (dfr) genes on the transfer frequencies of plasmids from environmental, antibioticresistant Escherichia coli. Of 51 sulfamethoxazole and trimethoprim-resistant E. coli isolates conferring at least one mob gene (mob P51 , mob F11 , mob F12 , mob Q11 , mob Q12 , or mob Qu ), 38 produced transconjugants with an overall mean frequency of 1.60 £ 10 ¡3 transconjugants/ donors (T/D) or 5.89 £ 10 ¡3 transconjugants/recipients (T/R). The presence or absence of intI1 and intI2 and the presence or absence of different targeted dfr genes (dfrA1, dfrA8, dfrA12, dfrA14, dfrA17, and/or dfrB3) were not statistically related to plasmid transfer frequencies as determined by ANOVA (P 0.05). However, E. coli isolates recovered 2 km downstream of wastewater treatment plant effluent input, and those possessing resistance to 3 antibiotics had significantly greater plasmid transfer frequency than their counterparts when calculated as T/D (ANOVA followed by Fisher's least significant difference means comparison, P < 0.05). Greater plasmid transfer frequency calculated as T/D was also measured for E. coli possessing 3 compared to a single sul gene. The invitro frequency suggests that horizontal gene transfer of conjugative mediated-antibiotic (sul) resistance genes may be significant among resistant, stream bacteria.

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Prevalence and characterization of integrons from bacteria isolated from a slaughterhouse wastewater treatment plant

Abstract: Our data support the hypothesis that WWTPs constitute a potential hot spot for horizontal gene transfer and for selection of antimicrobial resistance genes among aquatic bacteria. Moreover, water discharges represent a possible risk for dissemination of undesirable genetic traits.

Cited by 143 publications

References 28 publications

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

Monitoring of drug resistance amplification and attenuation with the use of tetracycline-resistant bacteria during wastewater treatment

Inadequately Treated Wastewater as a Source of Human Enteric Viruses in the Environment

Conjugative transmission of antibiotic-resistance from stream waterEscherichia colias related to number of sulfamethoxazole but not class 1 and 2 integrase genes

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