Prevalence and Characterization of Extended Spectrum Beta Lactamase Producing Escherichia coli from Broilers

Pal, Susmita; Dey, Samir; Batabyal, K.; Banerjee, Abhiroop; Joardar, Siddhartha Narayan; Samanta, Indranil; Isore, D.P.

doi:10.20546/ijcmas.2020.903.070

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…So it can be expected that combination of ayurveda and nanotechnology may provide the solution to resolve the antimicrobial resistance issue due to ESBL producing E. coli in poultry. Few research has reported that mineral rich Rasamanikya Nanoparticle (RMNP) which is ayurvedic herbometallic nanomedicine have antimicrobial potential assessed by in-vitro assay (Ruidas et al, 2019;Pal, et al,2020;Halder et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ayurvedic Nanoparticle Drug Rasamanikya against ESBL Producing Gastrointestinal Gram-negative Bacteria Escherichia coli in Experimental Poultry

Halder¹,

Sarkar²,

Bandapadhyay³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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The present study investigate the effects of oral administration of ayurvedic nanoparticle drug Rasamanikya against ESBL producing gastrointestinal gram-negative bacteria Escherichia coli in experimental broiler chicken and haemato-biochemical, pathological alteration due to drug administration. At the age of 21 days, the birds were divided into three groups i.e. group-C served as control group, group-E treated with Enrofloxacin which act as standard control group and group-RM treated with test drug Rasamanikya, containing six birds in each group. On the day 22, the experiment (Drug trial) was started and continued for next 28 days. On the day 0, 7, 14, 21 and 28 of experiment fecal sample was collected by using fecal swab from each bird of three groups to examine the ESBL producing gastrointestinal gram-negative bacterial count. Blood sample also be collected on day 0, 7, 14 and 28 of experiment from wing vein with the help of tuberculin syringe in sterile vial under aseptic condition to evaluate the haemato-biochemical parameters i.e. Hb%, SGPT, SGOT, ALP, Urea and Creatinine. After 28 days of experiment, at the age of 50th day, the birds were slaughtered to collect tissue sample from heart, lung, liver and kidney. Illeo-caecal junction tissue was also collected to examine the illeo-caecal coliform count. Fecal coliform count decreased significantly in RM treated group in comparison to control group. There was no significant (P< 0.01) alteration in hemoglobin percentage, uric acid and creatinine level but serum SGPT, SGOT and ALP increased significantly in RM treated group. The illeo-caecal coliform count was decreased significantly in standard control and Rasa Manikya treated groups in both dilutions 1:50 and 1:100 in comparison to normal control group. The result indicate that the test drug Rasamanikya able to decrease the fecal and illeo-caecal coliform count but histopathological findings clearly showed the drug causes chronic toxicity in birds of RM treated group.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Ayurvedic Nanoparticle Drug Rasamanikya against ESBL Producing Gastrointestinal Gram-negative Bacteria Escherichia coli in Experimental Poultry

Halder¹,

Sarkar²,

Bandapadhyay³

et al. 2022

Int.J.Curr.Microbiol.App.Sci

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Genetic diversity and risk factor analysis of drug-resistant Escherichia coli recovered from broiler chicken farms

Bhargavi

Sahu

Nishanth

et al. 2023

Comparative Immunology, Microbiology and Infectious Diseases

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Extended spectrum β-lactamase (ESBL) OF Escherichia coli resistance from the broiler cloaca in the traditional market banda aceh

Rastina,

Iskandar,

Zahra

2024

BIO Web Conf.

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Extended Spectrum β-Lactamase (ESBL)-producing Escherichia coli is a type of bacteria that can cause several infectious diseases in various animal and human tissues. ESBL bacteria have been reported to be resistant to various classes of antibiotics including penicillin, third-generation cephalosporins (cefotaxime, ceftriaxone and ceftazidime), and monobactam (aztreonam). This study focused on the presence of Escherichia coli as an ESBL producer in the cloaca of broiler. The purpose of this study was to determine the presence or absence of ESBL-producing Escherichia coli in the cloaca of broiler in the traditional market, Banda Aceh. This study used 47 samples of broiler cloacal swabs. The study used Cross-Sectional Observation and the disc diffusion methods. The presence of ESBL-producing Escherichia coli in cloacal swabs was identified using Eosin Methylene Blue Agar (EMBA) media. Detection of ESBL production using β-lactam antibiotic discs, namely cefotaxime, ceftazidime and ampicillin. The results showed the presence of ESBL-producing Escherichia coli bacteria isolated from cloacal swabs of broiler in the traditional market, Banda Aceh, which reached 21.27% (10/47). Sensitivity test results showed high resistance of Escherichia coli to cefotaxime 100% (10/10), ceftazidime 90% (9/10), and ampicillin 70% (7/10). Referring to CLSI, all isolates (100%) were ESBL-producing Escherichia coli. Based on the results of the study, it can be concluded that there is Extended Spectrum β-lactamase (ESBL) producing Escherichia coli in the cloaca of broiler in the traditional market, Banda Aceh.

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Prevalence and Characterization of Extended Spectrum Beta Lactamase Producing Escherichia coli from Broilers

Cited by 4 publications

References 12 publications

Evaluation of Ayurvedic Nanoparticle Drug Rasamanikya against ESBL Producing Gastrointestinal Gram-negative Bacteria Escherichia coli in Experimental Poultry

Evaluation of Ayurvedic Nanoparticle Drug Rasamanikya against ESBL Producing Gastrointestinal Gram-negative Bacteria Escherichia coli in Experimental Poultry

Genetic diversity and risk factor analysis of drug-resistant Escherichia coli recovered from broiler chicken farms

Extended spectrum β-lactamase (ESBL) OF Escherichia coli resistance from the broiler cloaca in the traditional market banda aceh

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