Prevalence and characterization of Campylobacter jejuni from chicken meat sold in French retail outlets

Guyard‐Nicodème, Muriel; Rivoal, Katell; Houard, Emmanuelle; Rose, Valérie; Quesne, Segolénè; Mourand, Gwénaëlle; Rouxel, Sandra; Kempf, Isabelle; Guillier, Laurent; Gauchard, Françoise; Chemaly, Marianne

doi:10.1016/j.ijfoodmicro.2015.02.013

Cited by 80 publications

(47 citation statements)

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“…1, which revealed that gentamicin resistance was much more prevalent in C. coli than in C. jejuni isolates (P Ͻ 0.0001, Fisher's exact test). Generally, the rate of resistance of Campylobacter to gentamicin is low (Ͻ2%) in other countries (8,18,19). However, recent studies performed in China suggested that the frequency of gentamicin resistance is high in Campylobacter, especially in C. coli isolated from swine and broiler chickens (23.2% to 95.4%) (10)(11)(12).…”

Section: Resultsmentioning

confidence: 99%

High Prevalence and Predominance of the aph(2″)-If Gene Conferring Aminoglycoside Resistance in Campylobacter

Yao

Liu

Wang

et al. 2017

Antimicrob Agents Chemother

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Campylobacter is a major foodborne pathogen, and previous studies revealed that Campylobacter isolates from food-producing animals are increasingly resistant to gentamicin in China. The molecular epidemiology and genetic mechanisms responsible for gentamicin resistance in China have not been well understood. In this study, 607 Campylobacter isolates of chicken and swine origins collected in 2014 were analyzed, revealing that 15.6% (25/160) of the Campylobacter jejuni isolates and 79.9% (357/447) of the Campylobacter coli isolates were resistant to gentamicin. PCR detection of the gentamicin resistance genes indicated that aph(2Љ)-If was more prevalent than the previously identified aacA/aphD gene and has become the dominant gentamicin resistance determinant in Campylobacter. Transformation and wholegenome sequencing as well as long-range PCR discovered that aph(2Љ)-If was located on a chromosomal segment inserted between two conserved genes, Cj0299 and panB. Cloning of aph(2Љ)-If into gentamicin-susceptible C. jejuni NCTC 11168 confirmed its function in conferring high-level resistance to gentamicin and kanamycin. Molecular typing by pulsed-field gel electrophoresis suggested that both regional expansion of a particular clone and horizontal transmission were involved in the dissemination of the aph(2Љ)-If gene in Campylobacter. To our knowledge, this is the first report describing the high prevalence of a chromosomally encoded aph(2Љ)-If gene in Campylobacter. The high prevalence and predominance of this gene might be driven by the use of aminoglycoside antibiotics in food animal production in China and potentially compromise the usefulness of gentamicin as a therapeutic agent for Campylobacter-associated systemic infection.

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Section: Resultsmentioning

confidence: 99%

High Prevalence and Predominance of the aph(2″)-If Gene Conferring Aminoglycoside Resistance in Campylobacter

Yao

Liu

Wang

et al. 2017

Antimicrob Agents Chemother

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“…The first sampling survey allowed the collection of isolates from ceca (n ϭ 11) and carcasses (n ϭ 21) collected from 425 batches of broiler chickens slaughtered in 58 French slaughterhouses over a 12-month period in 2008 (55). During the second monitoring survey, retail meat isolates (n ϭ 33) were collected from broiler meat sampled in retail outlets over a 6-month period in 2009 in geographic areas representing the most significant broiler meat consumption patterns in France (14,56). Cattle isolates were collected from dairy cow feces sampled during a local survey in 10 farms located in France in 2013 (n ϭ 13) (57).…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification of Host-Segregating Epidemiological Markers for Source Attribution in Campylobacter jejuni

Thépault

Méric

Rivoal

et al. 2017

Appl Environ Microbiol

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106

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Campylobacter is among the most common worldwide causes of bacterial gastroenteritis. This organism is part of the commensal microbiota of numerous host species, including livestock, and these animals constitute potential sources of human infection. Molecular typing approaches, especially multilocus sequence typing (MLST), have been used to attribute the source of human campylobacteriosis by quantifying the relative abundance of alleles at seven MLST loci among isolates from animal reservoirs and human infection, implicating chicken as a major infection source. The increasing availability of bacterial genomes provides data on allelic variation at loci across the genome, providing the potential to improve the discriminatory power of data for source attribution. Here we present a source attribution approach based on the identification of novel epidemiological markers among a reference pan-genome list of 1,810 genes identified by gene-by-gene comparison of 884 genomes of Campylobacter jejuni isolates from animal reservoirs, the environment, and clinical cases. Fifteen loci involved in metabolic activities, protein modification, signal transduction, and stress response or coding for hypothetical proteins were selected as host-segregating markers and used to attribute the source of 42 French and 281 United Kingdom clinical C. jejuni isolates. Consistent with previous studies of British campylobacteriosis, analyses performed using STRUCTURE software attributed 56.8% of British clinical cases to chicken, emphasizing the importance of this host reservoir as an infection source in the United Kingdom. However, among French clinical isolates, approximately equal proportions of isolates were attributed to chicken and ruminant reservoirs, suggesting possible differences in the relative importance of animal host reservoirs and indicating a benefit for further nationalscale attribution modeling to account for differences in production, behavior, and food consumption.IMPORTANCE Accurately quantifying the relative contribution of different host reservoirs to human Campylobacter infection is an ongoing challenge. This study, based on the development of a novel source attribution approach, provides the first results of source attribution in Campylobacter jejuni in France. A systematic analysis using gene-by-gene comparison of 884 genomes of C. jejuni isolates, with a pan-genome list of genes, identified 15 novel epidemiological markers for source attribution. The different proportions of French and United Kingdom clinical isolates attributed to

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“…(16) The hipO and asp Table 3. Relationship of subjects' contact with animals and poultry and place of habitation with Campylobacter species detected by culture and PCR (n=1010) PCR = polymerase chain reaction; C/P = contaminated based on PCR results/all subjects with certain age; PR= prevalence ratio genes were targeted in the present investigation, since the hipO gene has been represented as the most widely validated gene for the identification of C. jejuni and is highly conserved in C. jejuni strains, (26) while the asp gene is highly specific for C. coli and encodes aspartokinase. (34) Although several researches have targeted the rRNA genes for genus and species identification of Campylobacter they might have found a lower specificity due to the high level of conservation among closely related species.…”

Section: Discussionmentioning

confidence: 99%

“…(24) Laboratory methods for diagnosing Campylobacter enteritis have been successfully developed, such as polymerase chain reaction (PCR), genotyping methods like ribotyping, pulsed-field gel electrophoresis and ELISA for detecting DNA and antigens in stool samples and also specific culturing of the organism from fecal specimens. (25)(26)(27)(28) The ability of PCR to amplify minute amounts of specific microbial DNA sequences has made it a powerful molecular tool. (5,29) It is reported that multiplex PCR diagnostic tools are fast, inexpensive and sensitive for Campylobacter species.…”

Section: Original Article Introductionmentioning

confidence: 99%

Contact with poultry and animals increases risk of Campylobacter infections in adults of Ardabil province, Iran

Ranjbar

Babazadeh

2017

UnivMed

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Contact with poultry and animals increases risk of Campylobacter infections in adults of Ardabil province, Iran BACKGROUNDThe acute gastroenteritis caused by campylobacteriosis is known as one of the common infectious diseases with worldwide distribution. The aim of this study was to detect Campylobacter species in stool samples by routine culturing and polymerase chain reaction (PCR) and explore the risk factors in adult subjects in East Azerbaijan province of Iran. METHODSA cross sectional study involving 1010 adult subjects, from whom stool samples were collected. Samples with inflammatory criteria like fecal leukocytes (WBC ≥5) were selected and isolated through fecal lactoferrin detection test. The WBC ≥5 and lactoferrin positive samples were selected for Campylobacter detection by culture and PCR methods. The required information consisting of gender, age, place of habitation, and contact with poultry and animals were asked and recorded. Chi-square test and prevalence ratio (PR) was used to analyze the data. RESULTSOf 1010 stool samples, 231 (22.9%) had WBC ≥5, and from these samples 58 (25.1%) were positive by culturing and 61 (26.4%) by PCR. Subjects having habitual contact with animals and poultry had increased risk of Campylobacter infections by 1.65 times compared with subjects without contact with animals and poultry (PR=1.65; 95% CI: 1.07-2.68). CONCLUSIONSDetection of Campylobacter infections by PCR was more sensitive in adults. Investigation of Campylobacter prevalence in Ardabil showed this bacterium should be viewed as one of the possible pathogens in inflammatory diarrheal cases. People having habitual contact with animals should check the health of the animals regularly and not consume food from suspected sources.

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Prevalence and characterization of Campylobacter jejuni from chicken meat sold in French retail outlets

Cited by 80 publications

References 44 publications

High Prevalence and Predominance of the aph(2″)-If Gene Conferring Aminoglycoside Resistance in Campylobacter

High Prevalence and Predominance of the aph(2″)-If Gene Conferring Aminoglycoside Resistance in Campylobacter

Genome-Wide Identification of Host-Segregating Epidemiological Markers for Source Attribution in Campylobacter jejuni

Contact with poultry and animals increases risk of Campylobacter infections in adults of Ardabil province, Iran

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