Prevalence and carcinogenic risk of high-risk human papillomavirus subtypes in different cervical cytology: a study of 124,251 cases from the largest academic center in China

Tao, Xiang; Zhang, Huina; Wang, Shunni; Chen, Tingting; Cong, Qing; Wang, Li; Zhou, Xianrong; Zhao, Chengquan

doi:10.1016/j.jasc.2021.03.006

“…These results suggested that the cervical lesions in HPV16-positive women might develop into HSILs at a younger age or be diagnosed earlier than the cervical lesions in women with other hrHPV genotypes. Some studies reported a correlation between cervical HPV genotypes and cytological ndings in the general population, including the latest study in our clinical centre and another publication revealing the substantial correlation between eight HPV genotypes and cytological HSILs or worse (34,35). However, few publications report this correlation in speci c histopathological HSIL populations.…”

Section: Discussionmentioning

confidence: 89%

Visual Appearance of the Uterine Cervix Differs on the Basis of HPV Type Status in High-Grade Squamous Intraepithelial Lesion: The Results of a Reliable Method

Zhou

¹

,

Gong

²

,

Qiu

³

et al. 2021

Preprint

0

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Background This study aimed to evaluate the differences in cervical appearance among different human papillomavirus (HPV) genotypes in patients with high-grade squamous intraepithelial lesions (HSILs). Methods A total of 239 histopathological HSIL patients were included and divided into eight groups on the basis of HPV genotype in this prospective study. We present a reliable imaging method that provides reproducible, sensitive and unbiased assessments of cervical appearance characteristics. Colorimetric and morphometric data of colposcopic patterns after the application of acetic acid and iodine were acquired using ImageJ software and the surrounding normal regions were used as controls. Results The differences in red, green, blue and mean greyscale values in acetowhite epithelium obtained from ImageJ were not significant between the HPV16 and HPV18 groups (P < 0.05). The differences in red, green, and mean greyscale values in iodine staining were significant between the HPV18 and the other groups (P < 0.05). The frequency of the occurrence of the coarse mosaic patterns was significantly different among groups (P < 0.05), reducing in sequence were the HPV16, HPV-negative, HPV18, HPV31/33 and HPV52/58 groups. For the lesion area of HSILs, the HPV-negative group was the largest. The sensitivity of colposcopic impression varied among HPV genotypes (P < 0.01), being lowest in the HPV52 group. Conclusions Although being nonspecific, iodine negativity should be concerned in HPV18-positive lesions which is closely related to glandular epithelium. Vascular patterns in HPV52/58-positive HSIL are quite occult and tend to be missed by colposcopists. HPV-negative lesions are prone to be large and present typical vascular patterns despite being rare.

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“…These results suggested that the cervical lesions in HPV16-positive women might develop into HSILs at a younger age or be diagnosed earlier than the cervical lesions in women with other hrHPV genotypes. Some studies reported a correlation between cervical HPV genotypes and cytological findings in the general population, including the latest study in our clinical centre and another publication revealing the substantial correlation between eight HPV genotypes and cytological HSILs or worse [ 34 , 35 ]. However, few publications report this correlation in specific histopathological HSIL populations.…”

Section: Discussionmentioning

confidence: 99%

Visual appearance of the uterine cervix differs on the basis of HPV type status in high-grade squamous intraepithelial lesion: the results of a reliable method

Zhou

¹

,

Gong

²

,

Qiu

³

et al. 2022

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Background This study aimed to evaluate the differences in cervical appearance among different human papillomavirus (HPV) genotypes in patients with high-grade squamous intraepithelial lesions (HSILs). Methods A total of 239 histopathological HSIL patients were included and divided into eight groups on the basis of HPV genotype in this prospective study. We present a reliable imaging method that provides reproducible, sensitive and unbiased assessments of cervical appearance characteristics. Colorimetric and morphometric data of colposcopic patterns after the application of acetic acid and iodine were acquired using ImageJ software and the surrounding normal regions were used as controls. Results The differences in red, green, blue and mean greyscale values in acetowhite epithelium obtained from ImageJ were not significant between the HPV16 and HPV18 groups (P < 0.05). The differences in red, green, and mean greyscale values in iodine staining were significant between the HPV18 and the other groups (P < 0.05). The frequency of the occurrence of the coarse mosaic patterns was significantly different among groups (P < 0.05), reducing in sequence were the HPV16, HPV-negative, HPV18, HPV31/33 and HPV52/58 groups. For the lesion area of HSILs, the HPV-negative group was the largest. The sensitivity of colposcopic impression varied among HPV genotypes (P < 0.01), being lowest in the HPV52 group. Conclusions Although being nonspecific, iodine negativity should be concerned in HPV18-positive lesions which is closely related to glandular epithelium. Vascular patterns in HPV52/58-positive HSIL are quite occult and tend to be missed by colposcopists. HPV-negative lesions are prone to be large and present typical vascular patterns despite being rare.

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“…A meta-analysis of Arbyn et al showed an average HRHPV-positivity rate for ASC-US cytology of 43.0%, but it should be remembered that slightly lower levels compared to ours in this case might be associated with the use of a different HRHPV test, which detects less high-risk types [32] than in our study. Meanwhile, the data from large-scale research carried out by CAP-accredited Chinese laboratories reports 48.7-54.7% for ASC-US HRHPV-positive cases [33,34]. Our results also demonstrated similar positive HRHPV rates in women with LSIL (77.2%) as reported by American (74.4-85.3%) [35,36], other CAP-accredited laboratories outside the US (75.2-79.2%) [34,37], and Western European countries (76.0%) [32].…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, the data from large-scale research carried out by CAP-accredited Chinese laboratories reports 48.7-54.7% for ASC-US HRHPV-positive cases [33,34]. Our results also demonstrated similar positive HRHPV rates in women with LSIL (77.2%) as reported by American (74.4-85.3%) [35,36], other CAP-accredited laboratories outside the US (75.2-79.2%) [34,37], and Western European countries (76.0%) [32]. Similarities in HRHPV-positivity rates for major cervical screening abnormalities encompassing ASC-H, HSIL, and AGC (84.6%; 90.7% and 26.7%, respectively) also were noted-for American reports and other CAP-accredited laboratories outside the US (78.07%; 88.1-94.78% and 16.47-27.78%, respectively) [36,38].…”

Section: Discussionmentioning

confidence: 99%

Liquid-Based Screening Tests Results: HPV, Liquid-Based Cytology, and P16/Ki67 Dual-Staining in Private-Based Opportunistic Cervical Cancer Screening

Trzeszcz

¹

,

Mazurec

²

,

Jach

³

et al. 2021

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The baseline data from the private-based opportunistic cervical cancer screening with HRHPV14, liquid-based cytology (LBC) and p16/Ki67 testing, and its quality assessment/quality control (QA/QC) tools are lacking. The age-stratified analysis of 30,066 screening tests results in a Polish population, including the investigation of HRHPV14 status, LBC, and p16/Ki67 dual-staining reporting rates, along with immediate histopathologic correlations, was conducted. For cytopathologic QA/QC, the College of American Pathologists (CAP) benchmarks and enhanced safety protocol were used. The NILM/ASC-US/LSIL/ASC-H/HSIL/AGC reporting rates were 93.9/3.4/2.0/0.22/0.24/0.11, respectively, with correlating HRHPV14-positive rates of 8.4/48.9/77.2/84.6/90.7/26.7. The reporting rates for HSIL (CIN2+) in HRHPV-positive women with NILM/ASC-US/LSIL/ASC-H/HSIL/AGC referred for a colposcopy with biopsy were 19.1/25.8/22.5/12.4/19.1/1.1% of the total HSIL (CIN2+). In total, of the 1130 p16/Ki67 tests, 30% were positive. In NILM HRHPV14-positive women with available histology result, HSIL(CIN2+) was detected in 28.3% of cases. In the first such large-scale Polish study presenting HRHPV14, informed LBC and HSIL (CIN2+) results, the reporting rates were highly consistent with data from American and other CAP-certified laboratories, confirming the possibility of using the 2019 ASCCP risk-based guidelines as one of the screening strategies outside of the US, in conditions of proper QA/QC. The private-based screening model can be effective in cervical cancer prevention, particularly in countries with low population coverage of public funds-based systems.

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Prevalence and carcinogenic risk of high-risk human papillomavirus subtypes in different cervical cytology: a study of 124,251 cases from the largest academic center in China

Cited by 10 publications

References 21 publications

Visual Appearance of the Uterine Cervix Differs on the Basis of HPV Type Status in High-Grade Squamous Intraepithelial Lesion: The Results of a Reliable Method

Visual Appearance of the Uterine Cervix Differs on the Basis of HPV Type Status in High-Grade Squamous Intraepithelial Lesion: The Results of a Reliable Method

Visual appearance of the uterine cervix differs on the basis of HPV type status in high-grade squamous intraepithelial lesion: the results of a reliable method

Liquid-Based Screening Tests Results: HPV, Liquid-Based Cytology, and P16/Ki67 Dual-Staining in Private-Based Opportunistic Cervical Cancer Screening

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