Prevalence and antimicrobial susceptibility of virulent and avirulent multidrug-resistant Escherichia coli isolated from diarrheic neonatal calves

Barigye, Robert; Gautam, Ablesh; Piche, Lisa; Schaan, Lynn; Krogh, Darlene; Olet, Susan

doi:10.2460/ajvr.73.12.1944

Cited by 10 publications

(6 citation statements)

References 34 publications

(27 reference statements)

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“…The other enteric pathogens detected during this study were Salmonella spp., rotavirus and coronavirus. However, these findings are not surprising since these pathogens are universally reported in scouring calves worldwide ( Barigye et al, 2012 ; Cho and Yoon, 2014 ). As time had passed between the time of death and necropsy examination, the C. perfringens isolated from intestinal contents of two dead calves most likely reflect post-mortem proliferation of this organism.…”

Section: Discussionmentioning

confidence: 81%

“…In the present case report, E. coli was isolated from all the 36 faecal samples while the E. coli K99 antigen was only detected in 13 specimens. While E. coli K99 is an important cause of neonatal calf scours, other virulence factors including but not limited to F41, intimin, STa , Stx1 , Stx2 have also been reported in E. coli isolates from scouring neonatal calves ( Barigye et al, 2012 ; Ngeleka et al, 2019 ). While majority of the E. coli isolates from a bovine calf intestine are certainly normal flora, some of the K99-negative isolates cultured during the present study could have well been carrying other non-K99 virulence factors.…”

Section: Discussionmentioning

confidence: 99%

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Cryptosporidium parvum and other enteric pathogens in scouring neonatal dairy calves from the Al Ain region, United Arab Emirates

Mohteshamuddin

Hamdan

Alkaabi

et al. 2020

Veterinary Parasitology: Regional Studies and Reports

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Faecal specimens from 36 scouring neonatal calves from two dairy farms located in the Al Ain region of the UAE were screened with pathogen-specific antigen ELISA for Cryptosporidium parvum , Escherichia coli K99, rotavirus, and coronavirus. Additionally, faecal smears were stained with modified-acid-fast for Cryptosporidium oocysts, and the VITEK 2 system plus Gram's stain used to identify bacteria isolated from the faecal samples. Farm management practices were also evaluated during a farm visit. Of the 36 calves, 29, 13, 5, and 6 were positive for C. parvum , E. coli K99, bovine coronavirus, and rotavirus antigens respectively, while 27 were positive for Cryptosporidium oocysts. In various combinations, mixed infections were detected in 20/36 calves. This is the first report of C. parvum , E. coli K99, Salmonella spp., rotavirus, and coronavirus in ≤14-days-old scouring neonatal dairy calves from the UAE. Molecular characterization of these pathogens and nationwide epidemiological calf scour studies are recommended.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Cryptosporidium parvum and other enteric pathogens in scouring neonatal dairy calves from the Al Ain region, United Arab Emirates

Mohteshamuddin

Hamdan

Alkaabi

et al. 2020

Veterinary Parasitology: Regional Studies and Reports

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“…Agg was not detected in our isolates. Of the 60 (66.7%) isolates, 40 were GVI [1], and 34 of the GVI were asa1-gelE-ccf -positive. The other 20 (33.3%) isolates were NGVI.…”

Section: Resultsmentioning

confidence: 99%

“…Overnight bacterial culture was adjusted to 0.5 McFarland turbidity with the medium used to dilute the antibiotics, inoculated into gradient antibiotics plates and incubated at 35 ± 2°C for 18–24 hr. The results were assessed as previously described [3], and isolates resistant to ≥3 classes of the antibiotic drugs applied were categorized as multiple-drug-resistant isolates [1]. …”

Section: Methodsmentioning

confidence: 99%

Prevalence of virulence and resistance to antibiotics in pathogenic enterococci isolated from mastitic cows

Hou

Zhang

et al. 2016

The Journal of Veterinary Medical Science

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The prevalence of enterococci was examined in 280 milk samples collected from dairy cattle diagnosed with mastitis in three provinces of western China. Sixty strains of enterococci were isolated, and the species were determined based on their biochemical characters and 16S rRNA sequences. Resistance to seven antibiotic agents, frequency of seven virulence genes and pathogenicity in Kunming mice were tested to evaluate biological risks. The correlation between the number of virulence genes and pathogenicity in Kunming mice was also evaluated. The 60 isolates were allocated to Enterococcus hirae (68.3%), E. faecium (25.0%), E. mundtii (3.3%) and E. durans (3.3%). A total of 83.3% of the isolates were resistant to penicillin, whereas 15.0% were resistant to ampicillin, 15.0% to vancomycin, 6.7% to tetracycline and 25.0% to ciprofloxacin. Moreover, isolates exhibited 50.0% and 21.7% resistance to high levels of gentamycin and streptomycin, respectively. The gene asa1 was detected in all enterococcal isolates, whereas 66.7% of strains harbored three or more virulence factors and 56.7% were asa1-ccf-gelE-positive. In pathogenicity tests, isolates harboring numerous virulence factors did not show greater invasiveness than isolates harboring fewer virulence traits against Kunming mice. In conclusion, the number of virulence factors does not appear to predict the risk of enterococcal infection. Isolates were commonly resistant to penicillin and sporadically to ampicillin and vancomycin. These results suggest that the use of gentamycin, streptomycin and ciprofloxacin against enterococci should be avoided in mastitic cows. Additionally, the results demonstrate that the majority of isolates are sensitive to tetracycline.

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“…38 Recently, numerous studies have reported increases in multi-drug resistance of ETEC associated with antibiotic treated animal feed. [39][40][41][42] Majority of the fimbrial adhesins isolated from ETEC that infected farm animals expressed one or more unique adhesins. These adhesins also act as antigens/virulence factors.…”

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confidence: 99%

Synthesis and application of glycoconjugate-functionalized magnetic nanoparticles as potent anti-adhesion agents for reducing enterotoxigenic Escherichia coli infections

et al. 2015

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Polyethylene oxide stabilized magnetic nanoparticles (PEO-MNPs) bio-functionalized with glycoconjugate (Neu5Ac(α2-3)Gal(β1-4)Glcβ-sp) (GM3-MNPs) are synthesized using click chemistry. Interaction of GM3-MNPs with Enterotoxigenic Escherichia coli (ETEC) strain K99 (EC K99) is investigated using different microscopic techniques. Our results suggest that GM3-MNPs can effectively act as non-antibiotic anti-adhesion agents for treating ETEC infections.

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Prevalence and antimicrobial susceptibility of virulent and avirulent multidrug-resistant Escherichia coli isolated from diarrheic neonatal calves

Abstract: The prevalence of multidrug resistance was comparable for virulent and avirulent E coli isolated from diarrheic neonatal calves. Cephalosporins and aminoglycosides had reasonable susceptibility.

Cited by 10 publications

References 34 publications

Cryptosporidium parvum and other enteric pathogens in scouring neonatal dairy calves from the Al Ain region, United Arab Emirates

Cryptosporidium parvum and other enteric pathogens in scouring neonatal dairy calves from the Al Ain region, United Arab Emirates

Prevalence of virulence and resistance to antibiotics in pathogenic enterococci isolated from mastitic cows

Synthesis and application of glycoconjugate-functionalized magnetic nanoparticles as potent anti-adhesion agents for reducing enterotoxigenic Escherichia coli infections

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