Prevalence and antimicrobial susceptibility of Acinetobacter from raw bulk tank milk in Korea

Gurung, Mamata; Nam, Hyang-Mi; Tamang, Migma Dorji; Chae, Myeongju; Jang, Geum-Chan; Jung, Suk-Chan; Lim, Suk-Kyung

doi:10.3168/jds.2012-5965

Cited by 70 publications

(58 citation statements)

References 28 publications

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“…Similar results were found in cows with clinical mastitis and milk samples with mastitis in Korea (Nam et al 2009, Gurung et al 2013. The association of this bacterium with subclinical mastitis in buffaloes presents a new challenge for the treatment and control of disease.…”

Section: Discussionsupporting

confidence: 78%

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Vásquez-García

Silva²,

Almeida-Queiroz³

et al. 2017

Pesq. Vet. Bras.

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ABSTRACT.-Vásquez-García A., Silva T.S., Almeida-Queiroz S.R., Godoy S.H.S., Escherichia coli (4.5%), Serratia marcescens (4.0%), Stenotrophomonas maltophilia (4.0%), and low incidence: Klebsiella rhinoscleromatis (0.5%), Klebsiella ozaenae (0.5%), Tatumella ptyseos (0.5%), Enterobacter cloacae (0.5%). The molecular analysis indicated that samples positive by culture method of the genera Staphylococcus, Streptococcus and E. coli were positive by PCR. Para S. aureus and S. epidermidis the highest percentages of observed sensitivity were gentamicin (100%) and vancomycin (100%); for the genus Streptococcus to gentamicin and oxacillin and E. coli to Ampicilin. These findings may help in the control and treatment of subclinical mastitis in buffaloes and contribute to improving the efficiency and quality of the milk produced.

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Section: Discussionsupporting

confidence: 78%

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Vásquez-García

Silva²,

Almeida-Queiroz³

et al. 2017

Pesq. Vet. Bras.

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“…The bla OXA-143 gene has recently been detected and reported only in Brazil and South Korea (50). Until now, most of the A. baumannii populations detected outside hospitals were susceptible to antibiotics (21,27,(30)(31)(32). However, there have been growing concerns after the description of a bla OXA-23 gene from human stool and lice in Senegal (20); NDM-1-producing A. baumannii tk;4from a pig in China (55); and other carbapenemase-producing Acinetobacter spp.…”

Section: Discussionmentioning

confidence: 99%

“…Reports studying A. baumannii human carriage in the community are rare, and the prevalence has varied according to the countries and the identification methods used, from 0.5% to 3% in Europe (16)(17)(18), 4% in Hong Kong (19), and 5.4% in Senegal (20) to 10.4% in the United States (21). A. baumannii was also found in environments such as soil (22,23) and water (24,25) and in food products, such as vegetables (22,26), fish, meat (22), and raw bulk tank milk (27). Finally, in animals, the bacterium has been described as an emerging pathogen in veterinary clinics in Germany (28) and Switzerland (29).…”

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confidence: 99%

Extrahuman Epidemiology of Acinetobacter baumannii in Lebanon

Rafei

Hamzé

Pailhoriès

et al. 2015

Appl Environ Microbiol

102

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The presence of Acinetobacter baumannii outside hospitals is still a controversial issue. The objective of our study was to explore the extrahospital epidemiology of A. baumannii in Lebanon. From February 2012 to October 2013, a total of 73 water samples, 51 soil samples, 37 raw cow milk samples, 50 cow meat samples, 7 raw cheese samples, and 379 animal samples were analyzed by cultural methods for the presence of A. baumannii. Species identification was performed by rpoB gene sequencing. Antibiotic susceptibility was investigated, and the A. baumannii population was studied by two genotyping approaches: multilocus sequence typing (MLST) and bla OXA-51 sequence-based typing (SBT). A. baumannii was detected in 6.9% of water samples, 2.7% of milk samples, 8.0% of meat samples, 14.3% of cheese samples, and 7.7% of animal samples. All isolates showed a susceptible phenotype against most of the antibiotics tested and lacked carbapenemase-encoding genes, except one that harbored a bla OXA-143 gene. MLST analysis revealed the presence of 36 sequence types (STs), among which 24 were novel STs reported for the first time in this study. bla OXA-51 SBT showed the presence of 34 variants, among which 21 were novel and all were isolated from animal origins. Finally, 30 isolates had new partial rpoB sequences and were considered putative new Acinetobacter species. In conclusion, animals can be a potential reservoir for A. baumannii and the dissemination of new emerging carbapenemases. The roles of the novel animal clones identified in community-acquired infections should be investigated.

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“…Due to the ability of this organism to spread and to adapt to the nosocomial environment, it is important to identify and monitor the possible sources and routes of transmission to clinics. Within this context, we note that A. baumannii has been isolated from food products, such as milk (18), meat, fish, and raw vegetables (2,15,19). Among these, vegetables have been suspected to introduce A. baumannii into the clinical environment (2).…”

mentioning

confidence: 99%

“…Among these, vegetables have been suspected to introduce A. baumannii into the clinical environment (2). However, none of these investiga tions elucidated the link with the clinical context, and the A. baumannii strains were only tested for antibiotic resistance and not for their fingerprint (2,15,18,19). Overall, data about the clonality of A. baumannii from food are lacking, which precludes any speculation about the eventual exchange of A. baumannii clones between food and clinical settings.…”

mentioning

confidence: 99%

Antibiotic Resistance and Phylogenetic Characterization of Acinetobacter baumannii Strains Isolated from Commercial Raw Meat in Switzerland

Lupo¹,

Vogt²,

Seiffert³

et al. 2014

Journal of Food Protection

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The spread of antibiotic-resistant bacteria through food has become a major public health concern because some important human pathogens may be transferred via the food chain. Acinetobacter baumannii is one of the most life-threatening gram-negative pathogens; multidrug-resistant (MDR) clones of A. baumannii are spreading worldwide, causing outbreaks in hospitals. However, the role of raw meat as a reservoir of A. baumannii remains unexplored. In this study, we describe for the first time the antibiotic susceptibility and fingerprint (repetitive extragenic palindromic PCR [rep-PCR] profile and sequence types [STs]) of A. baumannii strains found in raw meat retailed in Switzerland. Our results indicate that A. baumannii was present in 62 (25.0%) of 248 (CI 95%: 19.7 to 30.9%) meat samples analyzed between November 2012 and May 2013, with those derived from poultry being the most contaminated (48.0% [CI 95%: 37.8 to 58.3%]). Thirty-nine strains were further tested for antibiotic susceptibility and clonality. Strains were frequently not susceptible (intermediate and/or resistant) to third- and fourth-generation cephalosporins for human use (i.e., ceftriaxone [65%], cefotaxime [32%], ceftazidime [5%], and cefepime [2.5%]). Resistance to piperacillin-tazobactam, ciprofloxacin, colistin, and tetracycline was sporadically observed (2.5, 2.5, 5, and 5%, respectively), whereas resistance to carbapenems was not found. The strains were genetically very diverse from each other and belonged to 29 different STs, forming 12 singletons and 6 clonal complexes (CCs), of which 3 were new (CC277, CC360, and CC347). RepPCR analysis further distinguished some strains of the same ST. Moreover, some A. baumannii strains from meat belonged to the clonal complexes CC32 and CC79, similar to the MDR isolates responsible for human infections. In conclusion, our findings suggest that raw meat represents a reservoir of MDR A. baumannii and may serve as a vector for the spread of these pathogens into both community and hospital settings.

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Prevalence and antimicrobial susceptibility of Acinetobacter from raw bulk tank milk in Korea

Cited by 70 publications

References 28 publications

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Extrahuman Epidemiology of Acinetobacter baumannii in Lebanon

Antibiotic Resistance and Phylogenetic Characterization of Acinetobacter baumannii Strains Isolated from Commercial Raw Meat in Switzerland

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