Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter Isolates from Commercial Broiler Flocks in Sokoto, Nigeria

Salihu, M. D.; Junaidu, A. U.; Magaji, A. A.; Yakubu, Yusuf

doi:10.3923/rjvs.2012.51.58

Cited by 22 publications

(25 citation statements)

References 21 publications

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“…Nearly similar percentage of 44.4% was reported in Italy (Pezzotti et al, 2003). Higher isolation rates of C. jejuni were also reported in different studies, in Nigeria (Salihu et al, 2012) and Malaysia (Mansouri-najand et al, 2012). However, lower prevalence rate of 31.4% was obtained in Reunion Island (Henry et al, 2011).…”

Section: Discussionsupporting

confidence: 71%

Bacteriological and Molecular Identification of Campylobacter Species in Chickens and Humans, at Zagazig City, Egypt

Tawab

Hofy

Ammar

et al. 2015

Benha Veterinary Medical Journal

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A B S T R A C TThe genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. A total of 533 samples from broiler chickens ;131 cloacal swabs, 39 chicken skin, 39 chicken cecal parts and 78 chicken meat thigh and breast meat (39 of each) were obtained from retail outlets, as well as, 246 stool swabs from persons attending the outpatient clinic of Al-Ahrar public hospital were examined. The isolation rate of Campylobacter species from chicken skin, thigh meat, breast meat, cecal parts, cloacal swabs and human stool samples were 30.8%, 38.5%, 30.8%, 41%, 35.1% and 5.3%, respectively. The conventional biochemical tests were used for discrimination between C. jejuni and other Campylobacter species based on standardized hippurate hydrolysis test. C. jejuni was isolated from cloacal swabs, skin, thigh meat, breast meat, cecal parts and human stool samples with the isolation rate of 45.7%, 50%, 46.7%, 41.7%, 81.3% and 76.9%, respectively. Real-Time PCR targeting hipO gene specific for C. jejuni was used for the confirmation of phenotypically identified 31 C. jejuni isolates. The results showed that the conventional culture methods and biochemical reactions were 100% in accordance with the results of PCR for identification and differentiation of C. jejuni.

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Section: Discussionsupporting

confidence: 71%

Bacteriological and Molecular Identification of Campylobacter Species in Chickens and Humans, at Zagazig City, Egypt

Tawab

Hofy

Ammar

et al. 2015

Benha Veterinary Medical Journal

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“…Campylobacter jejuni was the dominant species; (42, 79%), followed by C. coli (28, 14%), from the faeces and carcasses, respectively. This finding affirms the dominance of C. jejuni in poultry and poultry products (Jorgensen et al, 2002;Son et al, 2007;Salihu et al, 2012). Nevertheless, the high recovery of the thermophilic Campylobacters in comparison with the non-thermophiles could be imputed to the selective nature of the CampyGen gas generating system which optimizes the growth of Multidrug resistance defined as resistance to 3 or more antibiotics thermophilic Campylobacters but inhibits the growth of non-thermophiles by not producing hydrogen enriched atmosphere, which is required by the non-thermophilic Campylobacters (Workman et al, 2005).…”

Section: Discussionsupporting

confidence: 58%

“…This study recorded rate is however higher than the 14.1% earlier reported by Sackey et al (2001) but lower than the 43.6% by Abraham et al (1990) in Ghana. In South Africa, 47% has been reported in commercial and industrial broilers and 94% in industrial layers, 51.5% has been described in Nigeria, 63.8% in Cote d'Ivoire, 83.1% in Ireland and 87.2% in Poland (European Food Safety Authority, EFSA, 2010;Bester and Essack, 2012;Salihu et al, 2012;Bernadette et al, 2012;Wieczorek et al, 2012). Countries with low Campylobacter colonization rates in poultry has been attributed to limited small-scale poultry farms with high biosecurity levels which are measures lacking in our subregion (Johnsen et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Multidrug resistant Campylobacter in faecal and carcasses of commercially produced poultry

Karikari¹,

Obiri‐Danso²,

Frimpong³

et al. 2017

Afr. J. Microbiol. Res.

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Poultry meat and products are major transmission routes of human campylobacteriosis. The aim of this study was to determine the numbers and antibiogram profile of Campylobacter isolates from slaughtered broiler and layer birds. One hundred and sixty caecal and one hundred and thirty two carcasses were randomly sampled at the Kejetia poultry slaughter, isolated on charcoal-cefoperazonedeoxycholate agar (CCDA) and confirmed by API CAMPY and their resistance profiles assessed by Kirby-Bauer disk diffusion. Prevalence was 22.5 and 21.9% in the faecal and carcasses, respectively with no significant differences. Species identified among faecal isolates were Campylobacter jejuni (42%), Campylobacter coli (28%), Campylobacter lari (22%) and Campylobacter hyo-intestinalis (8%) while 79% C. jejuni, 14% C. coli, 4% C. jejuni sub sp. doylei, and 3% C. lari were obtained from the carcasses. Resistance to the β-lactams ranged from 75 to 100%, 41 to 86% to the quinolones, 14 to 36% to the aminoglycosides, 100% to erythromycin, 97 to 100% to tetracycline, 72 to 83% to chloramphenicol and 90 to 94% to trimethoprim sulfamethoxazole. All species were sensitive to imipenem, but 100% of isolates were multidrug resistant. Contamination of carcasses with multidrug resistant strains of Campylobacter is a threat to handlers and consumers and of major public health issue.

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“…These infections typically warrant treatment as well as infections of the immunosuppressed, pregnant women, children, elderly and those with recurrent symptoms; in such cases the macrolides and fluoroquinolones are the first line drugs prescribed [7] [8]. However, global reports of increasing resistance of Campylobacter to the drugs of choice and other clinically important antibiotics from human and animal origin are well established [3] [9] [10]. The resistance situation in developing countries such as Ghana might become worse as a result of widespread and unrestricted use of antibiotics as well as inadequate research into antimicrobial resistance.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance in <i>Campylobacter</i> Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana

Karikari¹,

Obiri‐Danso²,

Frimpong³

et al. 2017

OJMM

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Campylobacter is a leading causal agent of bacterial enteritis worldwide, but its prevalence is not well documented in Ghanaian hospitals. This study isolated Campylobacter species from patients with enteritis or urinary tract infections attending Komfo Anokye Teaching Hospital and assessed the antibiogram profile of isolated species. Two hundred and two (202) in-patients and outpatients samples of all age groups diagnosed with enteritis or UTI infections were analyzed from May 2013 to August 2013. Campylobacter species were detected using selective agar (mCCDA) and confirmed on API Campy system (bioMérieux, France), with disk diffusion method determined the resistance profile of the species. Of the 128 enteritis and 74 UTI patients samples analyzed, 26 and 9 isolates were respectively confirmed as Campylobacter spp. giving a prevalence of 17.3% (35/202). Species identified were C. jejuni (40%), C. jejuni sub sp. doylei (2.8%), C. coli (37%) and C. lari (20%). Resistance was 92.3% -100% each to erythromycin and the β-lactams, 61.5% -86.7% to trimethoprim sulfamethoxazole, 92.3% -93.3% to tetracycline, 46.2% -80% to chloramphenicol, 0% -60% to aminoglycosides and 0% to imipenem. Multidrug resistance of 97.1% was detected among species. Empirical treatment of Campylobacter enteritis with erythromycin and other common and cheap drugs may result in treatment failure in the face of high level resistance observed among the Campylobacter species.

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Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter Isolates from Commercial Broiler Flocks in Sokoto, Nigeria

Cited by 22 publications

References 21 publications

Bacteriological and Molecular Identification of Campylobacter Species in Chickens and Humans, at Zagazig City, Egypt

Bacteriological and Molecular Identification of Campylobacter Species in Chickens and Humans, at Zagazig City, Egypt

Multidrug resistant Campylobacter in faecal and carcasses of commercially produced poultry

Antibiotic Resistance in <i>Campylobacter</i> Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana

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Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter Isolates from Commercial Broiler Flocks in Sokoto, Nigeria

Cited by 22 publications

References 21 publications

Bacteriological and Molecular Identification of Campylobacter Species in Chickens and Humans, at Zagazig City, Egypt

Bacteriological and Molecular Identification of Campylobacter Species in Chickens and Humans, at Zagazig City, Egypt

Multidrug resistant Campylobacter in faecal and carcasses of commercially produced poultry

Antibiotic Resistance in &lt;i&gt;Campylobacter&lt;/i&gt; Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana

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Antibiotic Resistance in <i>Campylobacter</i> Isolated from Patients with Gastroenteritis in a Teaching Hospital in Ghana