Prevalence and antimicrobial resistance of Campylobacterfrom antibiotic-free broilers during organic and conventional processing

Bailey, Matthew; Taylor, Rhonda; Brar, Jagpinder Singh; Corkran, Sydney; Velasquez, C.; Rama, Estefanía Novoa; Oliver, Haley F.; Singh, Manpreet

doi:10.3382/ps/pey486

Cited by 29 publications

(26 citation statements)

References 12 publications

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“…The slow-growing yellow broilers were used in this study, as they account for about half share of broiler market in China and the slow-growing broilers are showing their promising values in organic agriculture in some western world, such as European Union (Fanatico et al, 2009). The birds used in this study may not represent all kinds of broiler breeds, including the fast-growing broiler, but could provide references in some extent.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Fecal Sampling as a Gut Proxy in the Study of Chicken Gut Microbiota

et al. 2019

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Despite the convenience and non-invasiveness of fecal sampling, the fecal microbiota does not fully represent that of the gastrointestinal (GI) tract, and the efficacy of fecal sampling to accurately represent the gut microbiota in birds is poorly understood. In this study, we aim to identify the efficacy of feces as a gut proxy in birds using chickens as a model. We collected 1,026 samples from 206 chickens, including duodenum, jejunum, ileum, cecum, and feces samples, for 16S rRNA amplicon sequencing analyses. In this study, the efficacy of feces as a gut proxy was partitioned to microbial community membership and community structure. Most taxa in the small intestine (84.11–87.28%) and ceca (99.39%) could be identified in feces. Microbial community membership was reflected with a gut anatomic feature, but community structure was not. Excluding shared microbes, the small intestine and ceca contributed 34.12 and 5.83% of the total fecal members, respectively. The composition of Firmicutes members in the small intestine and that of Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria members in the ceca could be well mirrored by the observations in fecal samples (ρ = 0.54–0.71 and 0.71–0.78, respectively, P < 0.001). However, there were few significant correlations for each genus between feces and each of the four gut segments, and these correlations were not high (ρ = −0.2–0.4, P < 0.05) for most genera. Our results suggest that fecal microbial community has a good potential to identify most taxa in the chicken gut and could moderately mirror the microbial structure in the intestine at the microbial population level with phylum specificity. However, it should be interpreted with caution by using feces as a proxy to study associations for microbial structure at individual microorganism level.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Fecal Sampling as a Gut Proxy in the Study of Chicken Gut Microbiota

et al. 2019

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“…However, in another American study no significant differences were observed in the prevalence of antimicrobial resistance between isolates collected from organic and conventional production except for tetracycline, for which isolates from organic production had a higher occurrence (82%) of resistance compared with conventional isolates (65%). However, in the latter study 75% of the isolates were resistant to at least one of the antimicrobials tested [38]. In a Danish study, considerable variation in the occurrence of nalidixic acid resistance in Campylobacter (ranging between 0 and 100% of isolates) was found between different farms [39].…”

Section: Discussionmentioning

confidence: 87%

“…It is unclear why Campylobacter strains isolated from organic chickens were resistant to ciprofloxacin and nalidixic acid to a significantly lower extent than those from conventionally raised chickens. Organic chickens are kept outdoors during the summer time, which is a season with high levels of Campylobacter among most animal species in the surroundings [37][38][39], providing potential exposure to many different strains, including resistant strains. Similar differences in the occurrence of resistance between C. jejuni from organic and conventionally produced chickens have been observed in an American study, where 2% of Campylobacter strains isolated from organically raised poultry were resistant to fluoroquinolones, while 46% and 67% of Campylobacter isolates from conventionally raised broilers and turkeys, respectively, were resistant to fluoroquinolones [26].…”

Section: Discussionmentioning

confidence: 99%

Differences in Genotype and Antimicrobial Resistance between Campylobacter spp. Isolated from Organic and Conventionally Produced Chickens in Sweden

et al. 2021

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Antibiotic resistance is a major challenge worldwide and increased resistance to quinolones in Campylobacter is being reported. Analysis of antibiotic resistance was performed on 157 Campylobacter strains (123 C. jejuni and 34 C. coli) from conventional and organic chickens produced in Sweden. Susceptibility for tetracycline, ciprofloxacin, erythromycin, nalidixic acid, streptomycin, and gentamycin was determined by microdilution. All 77 isolates from organic chickens were sensitive to all antibiotics, except two C. jejuni that were resistant to tetracycline. Of the 80 isolates from conventional chickens, 22.5% of C. jejuni and 11.1% of C. coli were resistant to quinolones and 5.6% of C. jejuni were resistant to tetracycline. Whole-genome sequencing resulted in 50 different sequence types of C. jejuni and six of C. coli. Nine sequence types were found in both organic and conventional chickens. Two of these (ST-19 and ST-257) included isolates from conventional broilers with different resistance phenotypes to the remaining isolates from conventional and organic broilers. There are management differences between the production systems, such as feed, breed, use of coccidiostats, and access to outdoor area. It is unlikely that quinolone resistance has arisen due to use of antimicrobials, since fluoroquinolones are not permitted in Swedish broiler production.

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“…10 In addition, numerous studies have reported very little difference in the shedding of drug-resistant bacterial strains between animals raised under organic or antimicrobial-free production systems. 11–14 Combined with studies such as that conducted by Hedman et al, these findings demonstrate the need to apply a One Health approach and study environmental reservoirs more closely, rather than focusing only on the resistance that arises following antimicrobial administration. 15–18…”

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confidence: 81%

The Mandate for a Global “One Health” Approach to Antimicrobial Resistance Surveillance

Thakur

Gray

2019

The American Journal of Tropical Medicine and Hygiene

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Prevalence and antimicrobial resistance of Campylobacterfrom antibiotic-free broilers during organic and conventional processing

Cited by 29 publications

References 12 publications

Efficacy of Fecal Sampling as a Gut Proxy in the Study of Chicken Gut Microbiota

Efficacy of Fecal Sampling as a Gut Proxy in the Study of Chicken Gut Microbiota

Differences in Genotype and Antimicrobial Resistance between Campylobacter spp. Isolated from Organic and Conventionally Produced Chickens in Sweden

The Mandate for a Global “One Health” Approach to Antimicrobial Resistance Surveillance

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