Prevalence and antibiotics susceptibility patterns of carbapenem resistant Enterobacteriaceae

Almugadam, Babiker Saad

doi:10.15406/jbmoa.2018.06.00201

Cited by 11 publications

(12 citation statements)

References 41 publications

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“…The first report of carbapenemase producer Enterobacteriaceae was reported in 1993 [ 4 ]. Multiple factors have contributed to an increase in rates of carbapenem resistance during the last decades; these factors include hospital policies like noncompliance with hand hygiene, inadequate isolation of infected patients and rising unnecessary carbapenem use along with inappropriate dosing and incorrect duration of antibiotic therapy [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Resistance to carbapenems can arise via different mechanisms, the most common being the production of carbapenemase (hydrolyzing carbapenems and other β-lactam antibiotics), poor binding to penicillin-binding proteins and efflux pumps [ 6 , 7 ]. Each of these mechanisms enables the organisms to resist to all or one of the carbapenem group (imipenem, meropenem, ertapenem, doripenem) and the third-generation cephalosporins, (ceftriaxone, cefotaxime, ceftazidime) [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Multicentre study of the main carbapenem resistance mechanisms in important members of the Enterobacteriaceae family in Iran

Armin

Fallah

Karimi

et al. 2021

New Microbes and New Infections

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Resistance to carbapenems has been increasingly reported from the Enterobacteriaceae family, with different mechanisms in different geographic parts of the world. This study investigated the mechanisms of carbapenem resistance in Escherichia coli, Klebsiella pneumoniae and Enterobacter spp. carried out as a multicentre study ( n = 10). All third-generation cephalosporin-resistant E. coli, K. pneumoniae and Enterobacter spp. that had been recovered from the selected provinces were included. Modified Hodge test and Carba NP test were done as a phenotypical method for detection of carbapenemase; the most common carbapenemase was detected by PCR. We evaluated the presence of an active efflux pump by using cyanide 3-chlorophenylhydrazone. Overexpression of AcrA/B and presence of OqxAB was detected by real-time PCR and conventional PCR respectively. Microorganisms in this study included 58 E. coli , 95 K. pneumoniae and 60 Enterobacter spp. Modified Hodge test showed a sensitivity of 41% and a specificity of 83%, and the Carba NP test showed a sensitivity of 26% and a specificity of 92% for detection of carbapenemase. OXA-48 was the most frequently detected carbapenemase, followed by NDM-1. Thirty-nine percent and 27% of positive cyanide 3-chlorophenylhydrazone test organisms included active AcrA/B and OqxAB efflux pumps respectively. The result showed the Carba NP test was more specific than MHT. Data confirmed the involvement of AcrA/B and OqxAB efflux pump as a carbapenem resistance mechanism in selected bacteria. Similar to other reports from the Middle East, we found OXA-48 and NDM-1 to be the most frequent carbapenemase.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multicentre study of the main carbapenem resistance mechanisms in important members of the Enterobacteriaceae family in Iran

Armin

Fallah

Karimi

et al. 2021

New Microbes and New Infections

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“…MBL producing bacteria showed high resistance to commonly used antibiotics especially of class Carbapenems, Cephalosporin, Monobactams, and Quinolones. (34)…”

Section: Resultsmentioning

confidence: 99%

Phenotypic Detection of Metallo-Β-Lactamase Mediated Carbapenem Resistance in Gram Negative Bacilli of Family Enterobacteriaceae in a Tertiary Care Hospital in North India

2020

jcr

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Background: This study was undertaken to determine the prevalence of Metallo-β-lactamase (MBL) producing gram negative bacteria in a tertiary care hospital in North India. Metallo-β-lactamases are β-lactamase enzymes with distinct ability to hydrolyze and confer resistance to the carbapenems which are the commonly used drugs for the treatment of infections caused by multidrug resistant gram negative bacteria. Pathogenic bacteria producing Metallo-β-lactamases are responsible for infections in tertiary care hospitals around the world and carbapenem resistance due to these enzymes have been reported over the past few years. Material and Methods: A total of 400 Enterobacteriaceae isolates from various clinical samples were identified and tested for susceptibility to various antibiotics by disc diffusion method. MBL detection was done by combined disc diffusion test and E-test. Result: The isolates included, E.coli 170(42.50%), K. pneumoniae 113(28.25%), Citrobacter spp. 90(22.50%) & Proteus spp. 27(6.25%).Out of the 400 Enterobacteriaceae 77 were carbapenem resistant of these 77 isolates 42 (54.54%) showed an increase in zone size by the combined disc diffusion test and E-test. MBL was detected in 27(30.33%) isolates of Citrobacter spp, 26 (23.0%) K. pneumoniae, 22 (12.94%) E.coli, 2 (7.40%) Proteus spp isolates. Conclusion: The study highlights the problem of MBL mediated resistance, which indicates therapeutic challenge for the clinicians and microbiologists. Simple disc method can be routinely employed to detect these common resistance mechanisms which will reduce the mortality and also spread of such resistant strains. In view of the increasing resistance of gram negative bacilli to carbapenems, rational use of antibiotics needs to be emphasised.

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“…The Enterobacteriaceae is a family of Gram-negative, facultative anaerobic bacteria that do not form spores, they leave a wide range of carbohydrates, have a complex antigenic structure, and produce a variety of toxins and other virulence factors. Some enteric organisms are part of the normal microbiota and eventually cause disease, but others are regularly pathogenic to humans [6,7].…”

Section: Introductionmentioning

confidence: 99%

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

et al. 2020

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Bacterial resistance has become an important public health problem. Bacteria have been acquiring mechanisms to resist the action of antimicrobial active pharmaceutical ingredients (API). Based on this, a promising alternative is the use of nanotechnology, since when the systems are presented in nanometric size, there is an increase in the interaction and concentration of the action at the target site improving the activity. Thus, this study aims to develop a polymeric nanoparticle (PN) composed of chitosan and hydroxypropylmethylcellulose, as an innovative strategy for the administration of an association between ceftriaxone and extract of S. brasiliensis, for the treatment of Enterobacteriaceae. From a Box–Behnken design, nanoparticles were obtained and evaluated using the DLS technique, obtaining the particle size between 440 and 1660 nm, IPD from 0.42 to 0.92, and positive charges. Morphological characteristics of PN by SEM revealed spherical morphology and sizes similar to DLS. Infrared spectroscopy showed no chemical interaction between the components of the formulation. The broth microdilution technique evaluated their antimicrobial activity, and a considerable improvement in the activity of the extract and the API compared to the free compounds was found, reaching an improvement of 133 times in the minimum inhibitory activity CRO.

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Prevalence and antibiotics susceptibility patterns of carbapenem resistant Enterobacteriaceae

Cited by 11 publications

References 41 publications

Multicentre study of the main carbapenem resistance mechanisms in important members of the Enterobacteriaceae family in Iran

Multicentre study of the main carbapenem resistance mechanisms in important members of the Enterobacteriaceae family in Iran

Phenotypic Detection of Metallo-Β-Lactamase Mediated Carbapenem Resistance in Gram Negative Bacilli of Family Enterobacteriaceae in a Tertiary Care Hospital in North India

Polymeric Nanoparticle Associated with Ceftriaxone and Extract of Schinopsis Brasiliensis Engler against Multiresistant Enterobacteria

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