Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods

Pesavento, Giovanna; Ducci, Barbara; Nieri, D.; Comodo, Nicola; Nostro, Antonella Lo

doi:10.1016/j.foodcont.2009.10.012

Cited by 144 publications

(152 citation statements)

References 36 publications

Supporting

Mentioning

117

Contrasting

Unclassified

Order By: Relevance

“…is possible and can be due to acquisition of a replicon originating in enterococci-streptococci. It is known that microorganisms (bacteria) can either acquire, or transfer antibiotic resistance genes from plasmids and transposons of other bacterial species (including Enterococci spp) either in vitro or in vivo in the intestinal tract (29,28). This is very important for their pathogenicity as bacteria that acquire new resistance are not disrupted by antibiotics during therapy.…”

Section: Discussion/conclusionmentioning

confidence: 99%

“…This may be due to the fact that Listeria sp. can transfer resistance genes to other enteric bacteria that become resistant to these antibiotics normally used in treatment of its infections as ampicillin is one of the first choice drugs for the treatment of listeriosis (28). The results showed a high level of multiresistance and all isolated strains were susceptible to only one of the first choice antibiotics used in listeriosis therapy (tetracycline) and to cotrimoxazole used as second choice antibiotic in treatment of listeriosis especially in patients allergic to penicillin (30,31,32).…”

Section: Discussion/conclusionmentioning

confidence: 99%

“…Listeria monocytogenes is slowly becoming resistant because of the uptake of resistance genes from other Gram-positive bacteria such as Listeria spp., Staphylococcus spp. and Enterococcus spp..A continued surveillance of emerging antimicrobial resistance of this pathogen is therefore important to ensure an effective treatment of human listeriosis (28). Bacterial resistances to antimicrobials have been demonstrated from the pre-antibiotic era and from remote environments with no antimicrobial exposure (33).…”

Section: Discussion/conclusionmentioning

confidence: 99%

“…Early studies that demonstrated this association and the possibility of the transfer of resistance between bacteria were undertaken in Japan in the 1950s. Studies with different bacterial pathogens showed that transfer of resistance was a common event (28). The developments in molecular biology enabled the genetic basis of this transfer, by extra chromosomal DNA (Rfactor or plasmids) to be explained (29).…”

Section: Discussion/conclusionmentioning

confidence: 99%

See 3 more Smart Citations

Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria

Uwanibe¹

2014

AVS

View full text Add to dashboard Cite

Abstract:Aims: To determine the presence of plasmids in Listeria monocytogenes strains isolated from fresh raw milk from healthy cattle in Nigeria and also determine its role in antibiotics resistance since this organism is a food borne pathogen of public health importance. Methods and results: Isolation of Listeria monocytogenes was done using selective enrichment and isolation protocol, as recommended by United States Department of Agriculture (USDA) and Association of Analytical Chemists/ International Dairy Federation (AOAC/ IDF). Identification was by phenotypic characterization and confirmed using β-haemolytic activity, phosphatidyl inositol phospholipase-C (PIPLC) assay and polymerase chain reaction (PCR) . Strains identified as Listeria monocytogenes from the six zones were determined and results expressed as rate of prevalence in graph. Twenty-three strains of Listeria monocytogenes isolated from 305 samples of fresh raw milk were screened for the presence of plasmid DNA using the method of Ehrenfeld and Clewel and agarose gel electrophoresis. One isolate was found to harbour one plasmid with molecular weight of 14.62kb. The plasmid was cured using different concentrations of acridine orange. Antibiotic sensitivity test was carried out on both the wild strain (with plasmid) and cured strain using disc diffusion method of Bauer-Kirby and it was found that the wild strain was resistant to seven of the twelve antibiotics used while the cured strain was resistant to only one. Conclusions: There is a possibility of the presence of strains of Listeria monocytogenes that can harbour plasmids. This could confer antibiotic resistance to the strains and thus make antibiotic therapy unsuccessful. The increase in the number of antibiotics which the cured strain of Listeria monocytogenes was susceptible to in this study showed that plasmid may play a significant role in antibiotics resistance. The Fulani herdsmen who take fresh raw milk without boiling or pasteurization may serve as carriers of resistant strains of Listeria monocytogenes and help in its spread in the environment through faecal pollution.

show abstract

Section: Discussion/conclusionmentioning

confidence: 99%

Section: Discussion/conclusionmentioning

confidence: 99%

Section: Discussion/conclusionmentioning

confidence: 99%

Section: Discussion/conclusionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria

Uwanibe¹

2014

AVS

View full text Add to dashboard Cite

show abstract

“…L. monocytogenes was found prevalently high in poultry (24.5%), intermediate in beef (24.4%) and less prevalent in pork (21.4%). Prevalence of L. monocytogenes in the eight isolated strains from ham and sandwiches was 37.5% and 25.0% respectively in Italy (Pesavento et al, 2010).…”

Section: Introductionmentioning

confidence: 92%

Detection of Emerging Food Pathogens in Chicken Meat Using Multiplex Polymerase Chain Reaction

Raja¹,

Rao²,

Babu³

et al. 2016

JAS

View full text Add to dashboard Cite

A study was undertaken to develop a multiplex PCR (m-PCR) protocol for simultaneous detection of Campylobacter jejuni and Listeria monocytogenes in chicken meat. The extraction of DNA was carried out using commercial DNA extraction kit, Phenol Chloroform and boiling method. Samples with OD ratio (260:280) between 1.7 and 1.9 were considered good in terms of concentration and purity and were used for PCR amplification. DNA extraction kit and Phenol Chloroform method revealed good OD value were used for sample extraction process. PCR and m-PCR amplification was carried out using genus specific primers were designed by targeting its Hyp (500 bp) and prfA (290 bp) gene for Campylobacter jejuni and Listeria monocytogenes respectively. Electrophoresis of amplified PCR products and gel documentation revealed 500 bp and 290 bp in 2% Agarose. The multiplex PCR technique was standardized using the reference strains with the similar amplification procedure. The minimum detection level (sensitivity) by mPCR for Campylobacter jejuni and Listeria monocytogenes was found to be 0.2 ng/μl and 1.0 ng/μl of DNA in a reaction mixture (25 μl). The developed multiplex PCR technique could detect Campylobacter jejuni and Listeria monocytogenes upto 3 × 10 5 and 3 × 10 4 CFU/ml of artificially inoculated meat homogenate. Around 60 chicken meat samples were collected from different regions of chennai and were screened for the presence of Campylobacter jejuni and Listeria monocytogenes. All the samples screened were not positive either for Campylobacter jejuni and Listeria monocytogenes. The negative samples were further checked by culture methods and good correlation between these two methods was observed. Hence, the m-PCR technique developed in this study can be used as a rapid screening test for detection of Campylobacter jejuni and Listeria monocytogenes from chicken meat within 24 hours.

show abstract

What Is the Problem with L. monocytogenes?

Jordan

Leong

Ordóñez

2015

Listeria Monocytogenes in the Food Processing Environment

View full text Add to dashboard Cite

Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods

Cited by 144 publications

References 36 publications

Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria

Detection of Plasmid in Listeria monocytogenes Isolated from Fresh Raw Milk in Nigeria

Detection of Emerging Food Pathogens in Chicken Meat Using Multiplex Polymerase Chain Reaction

What Is the Problem with L. monocytogenes?

Contact Info

Product

Resources

About