Prevalence and antibiotic resistance pattern of <i>Salmonella</i> serovars in integrated crop‐livestock farms and their products sold in local markets

Peng, Mengfei; Biswas, Debabrata; Almario, Jose Alejandro; Tesfaye, Bezait; Buchanan, Robert L.; Biswas, Debabrata

doi:10.1111/1462-2920.13265

Cited by 41 publications

(60 citation statements)

References 46 publications

(54 reference statements)

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“…In most cases, Salmonella infections are associated with diarrhea, weight loss, dramatic alterations in composition of blood cells, as well as death [12,40–42]. Accordantly we detected 10 5 -10 7 CFU intestinal colonization of ST induced salmonellosis and caused around 8% weight loss, 52% higher level of RBC, 19% and 71% lower levels of WBC (especially neutrophils and lymphocytes) and PLT, and severe cecal inflammation in the survival mice.…”

Section: Discussionsupporting

confidence: 76%

“…Salmonella and diarrheagenic Escherichia coli generally infect human gut intestine through consumption of contaminated foods and/or drinks [10–12]. Once these Gram-negative enteric pathogenic bacteria arrive in host gut, their complex type III secretion systems are activated, enabling them to introduce effector proteins directly into cell cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

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Prevention of enteric bacterial infections and modulation of gut microbiota composition with conjugated linoleic acids producingLactobacillusin mice

Peng

Tabashsum

Patel

et al. 2019

Preprint

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25Probiotics are recognized to outcompete pathogenic bacteria by receptor-mediated 26 colonizing and secreting functional metabolites which have direct antimicrobial activities 27 towards pathogens and/or improving host's gut health and immunity. We have constructed 28 a Lactobacillus casei (LC) probiotic strain, LC +mcra , by inserting mcra (myosin cross-29 reactive antigen) gene, which stimulates the conversion of conjugated linoleic acids. In this 30 study, we evaluated the protective roles of LC +mcra against pathogenic Salmonella enterica 31 serovar Typhimurium (ST) and enterohaemorrhagic E. coli (EHEC) infection in BALB/cJ 32 mice. Through a series of in vivo investigation, we observed that LC +mcra colonized 33 efficiently in mice gut and competitively reduced the infection with ST and EHEC in 34 various locations of small and large intestine, specifically cecum, jejunum, and ileum 35 (p<0.05). The cecal microbiota in ST-challenged mice with LC +mcra protection were 36 positively modulated with higher relative abundances Firmicutes but lower Proteobacteria 37 plus increased bacterial species diversity/richness based on 16S metagenomic sequencing. 38 Based on cytokine gene expression analysis by qRT-PCR, mice pretreated with LC +mcra 39 were found with attenuated bacterial pathogen-induced gut inflammation. Furthermore, 40 mice fed LC +mcra daily for one week could protect themselves from the impairments caused 41 by enteric infections with ST or EHEC. These impairments include weight loss, negative 42 hematological changes, intestinal histological alterations, and potential death. This in vivo 43 study suggests that daily consumption of novel conjugated linoleic acids over-producing 44 probiotic might be efficient in improving gut intestinal microbiome composition and 45 preventing/combating foodborne enteric bacterial infections with pathogenic Salmonella 46 and diarrheagenic E. coli. 3 47 48Author summary 49 Numerous bacteria colonize throughout the gastrointestinal tract and form a complex 50 microbial ecosystem known as gut microbiota. A balanced microbial composition is crucial 51 for maintaining proper gut health and host defense against pathogenic microbes. However, 52 enteric bacterial infections could cause illness and even lead to death of host when 53 foodborne pathogens like Salmonella and enterohaemorrhagic E. coli (EHEC) invade gut 54 intestine and cause imbalance of gut microbiota. Beneficial microbes in gastrointestinal 55 tract such as Lactobacillus and their secreted bio-active metabolites, are potential bio-56 agents to improve gut immunity and outcompete bacterial pathogens. In this study, to 57 evaluate roles of novel Lactobacillus strain LC +mcra which produce higher amount of a 58 group of beneficial secondary metabolites called conjugated linoleic acids, we have shown 59 that daily oral administration of this LC +mcra for one-week in mice lead to higher proportion 60 of beneficial bacterial colonization in different locations of intestine and a significant 61 reduction of path...

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Prevention of enteric bacterial infections and modulation of gut microbiota composition with conjugated linoleic acids producingLactobacillusin mice

Peng

Tabashsum

Patel

et al. 2019

Preprint

Self Cite

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show abstract

“…Our previous study demonstrated the potential for Salmonella risks with both conventional and mixed crop-livestock (MCL) farm systems. A higher overall prevalence of Salmonella contamination was detected in the MCL system, whereas multiantibiotic resistance in Salmonella was found predominantly in conventional farms, where farmers are allowed to use synthetic antibiotics, and in their related postharvest products in retail stores (25). In this study, we further investigated phylogenetically inferred relatedness, genomic characteristics, and the alteration of antibiotic resistance-related genes under environmental pressures of the designated S. Typhimurium isolates.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that pasture/organic farming cannot meet the demand for animal products for seven billion people. Furthermore, solely blocking or withdrawing subtherapeutic antibiotic use in animal farming without controlling therapeutic or higher doses of antibiotics and use of other chemicals might not be enough to mitigate the threat of rising rates of antibiotic-resistant zoonotic pathogenic bacteria (9,25,26). According to published data from the Centers for Disease Control and Prevention (CDC), multidrug resistance in Salmonella accounts for approximately 6,200 cases (10% of total) of Salmonella infections each year in the United States.…”

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confidence: 99%

Alterations of Salmonella enterica Serovar Typhimurium Antibiotic Resistance under Environmental Pressure

Peng

Biswas

Buchanan

2018

Appl Environ Microbiol

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Microbial horizontal gene transfer is a continuous process that shapes bacterial genomic adaptation to the environment and the composition of concurrent microbial ecology. This includes the potential impact of synthetic antibiotic utilization in farm animal production on overall antibiotic resistance issues; however, the mechanisms behind the evolution of microbial communities are not fully understood. We explored potential mechanisms by experimentally examining the relatedness of phylogenetic inference between multidrug-resistant serovar Typhimurium isolates and pathogenic Typhimurium strains based on genome-wide single-nucleotide polymorphism (SNP) comparisons. Antibiotic-resistant Typhimurium isolates in a simulated farm environment barely lost their resistance, whereas sensitive Typhimurium isolates in soils gradually acquired higher tetracycline resistance under antibiotic pressure and manipulated differential expression of antibiotic-resistant genes. The expeditious development of antibiotic resistance and the ensuing genetic alterations in antimicrobial resistance genes in Typhimurium warrant effective actions to control the dissemination of antibiotic resistance. Antibiotic resistance is attributed to the misuse or overuse of antibiotics in agriculture, and antibiotic resistance genes can also be transferred to bacteria under environmental stress. In this study, we report a unidirectional alteration in antibiotic resistance from susceptibility to increased resistance. Highly sensitive serovar Typhimurium isolates from organic farm systems quickly acquired tetracycline resistance under antibiotic pressure in simulated farm soil environments within 2 weeks, with expression of antibiotic resistance-related genes that was significantly upregulated. Conversely, originally resistant Typhimurium isolates from conventional farm systems lost little of their resistance when transferred to environments without antibiotic pressure. Additionally, multidrug-resistant Typhimurium isolates genetically shared relevancy with pathogenic Typhimurium isolates, whereas susceptible isolates clustered with nonpathogenic strains. These results provide detailed discussion and explanation about the genetic alterations and simultaneous acquisition of antibiotic resistance in Typhimurium in agricultural environments.

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“…Early detection and management of potentially pathogenic Salmonella spp. is an essential step towards prevention and management of Salmonellosis (Peng et al, 2016).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Biofilm formation by Salmonella sp. in the poultry industry: Detection, control and eradication strategies

Merino

Procura

Trejo

et al. 2019

Food Research International

102

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Prevalence and antibiotic resistance pattern of Salmonella serovars in integrated crop‐livestock farms and their products sold in local markets

Cited by 41 publications

References 46 publications

Prevention of enteric bacterial infections and modulation of gut microbiota composition with conjugated linoleic acids producingLactobacillusin mice

Prevention of enteric bacterial infections and modulation of gut microbiota composition with conjugated linoleic acids producingLactobacillusin mice

Alterations of Salmonella enterica Serovar Typhimurium Antibiotic Resistance under Environmental Pressure

Biofilm formation by Salmonella sp. in the poultry industry: Detection, control and eradication strategies

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