Prevalence and antibiogram of coagulase negative Staphylococci in bioaerosols from different indoors of a university in India

Kumari, Himani; Chakraborti, Trina; Singh, Madhuri; Chakrawarti, Maneet Kumar; Mukhopadhyay, Kasturi

doi:10.1186/s12866-020-01875-8

Cited by 8 publications

(7 citation statements)

References 53 publications

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“…Staphylococcus was also the dominant bacteria group in sports facilities at the Centre of Physical Culture and Sport at the University in Northern Poland [12]. This bacteria genus may particularly affect the health of students [55].…”

Section: Multi Antibiotic Resistance Test (Mar) Of Bacterial Aerosol (Ba) In a Highschool Gym Before Gym Classes (Bgc) And During Gym Clamentioning

confidence: 99%

Microbiological Air Quality in a Highschool Gym Located in an Urban Area of Southern Poland—Preliminary Research

2020

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The benefits of regular exercise include improved physical and mental health. The school gym is a particular micro-environment where students perform intensive physical training. The question is if there is an increased risk of microbiological contamination. This preliminary work studied the exposure of students to bacterial aerosol (BA) in a highschool gym located in an urban area of Southern Poland. A sampling of BA was undertaken with an Andersen six-stage impactor (ANDI). BA was identified using API (analytical profile index) tests. The BA concentrations were expressed as Colony Forming Units (CFU) per cubic metre of air. The results showed that before gym classes (BGC), the concentration of BA was 4.20 × 102 ± 49.19 CFU/m3, while during gym classes (DGC), the level of BA more than doubled (8.75 × 102 ± 121.39 CFU/m3). There was also an increase in the respirable fraction of BA (particles less than 3.3 µm). Before the start of the sports activities, respirable fraction accounted for 30% of the BA, while during physical education classes, this share increased to over 80%. Identification of BA species showed that the dominant group of bacteria in the indoor air of the gym BGC was Gram-positive rods (61%) and for DGC it was Gram-positive cocci (81%). We detected that one bacteria strain (Corynebacterium striatum) was classified into risk group 2 (RG2) according to Directive 2000/54/EC. Additionally, multi-antibiotic resistance (MAR) showed that among the isolated airborne bacteria, the highest antibiotic resistance was demonstrated by Staphylococcus epidermis (isolated DGC) and Pseudomonas sp. (isolated BGC). The quantitative and qualitative information on microbiological air quality (MIAQ) in the school gym indicates that the actions to improve indoor physical activity spaces are recommended.

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Section: Multi Antibiotic Resistance Test (Mar) Of Bacterial Aerosol (Ba) In a Highschool Gym Before Gym Classes (Bgc) And During Gym Clamentioning

confidence: 99%

Microbiological Air Quality in a Highschool Gym Located in an Urban Area of Southern Poland—Preliminary Research

2020

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“…The coagulase test involved aseptically inserting 200 μl of plasma into a sterile microtube. Then add 3-4 culture colonies and incubate the mixture at 37°C for 24 hours to detect the presence of free coagulase (Kumari et al 2020).…”

Section: Isolation and Identification Of Staphylococcus Aureusmentioning

confidence: 99%

Detection of multidrug-resistant Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia

PUTRA

Khairullah²,

Effendi³

et al. 2023

Biodiversitas

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Abstract. Putra GDS, Khairullah AR, Effendi MH, Lazuardi M, Kurniawan SC, Afnani DA, Silaen OSM, Waruwu YKK, Millannia SK, Widodo A, Ramadhani S, Farizqi MTI, Riwu KHP. 2023. Detection of multidrug-resistant (MDR) Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia. Biodiversitas 24: 423-430. Bacterial resistance has emerged as a major concern in dairy farms in Indonesia due to the pervasive usage of antibiotics. Furthermore, no specific research has been done to explain the prevalence of Staphylococcus aureus in isolated milk from dairy cows in Kediri, particularly in Medowo Village, and their antibiotic resistance. Moreover, to control the emergence of diseases in humans brought on by dairy cow's milk or infections transmitted through milk, additional research on the prevalence and resistance of bacteria in dairy farms in the Kediri district is urgently required. In Medowo, the Village Kandangan of District, Kediri Regency, Indonesia, 100 dairy cow's milk samples were taken from farms in numerous hamlets. The Kirby-Bauer method performed an antibiotic sensitivity test using disk diffusion. The sensitivity test was attached with antibiotic discs on tetracycline, penicillin, gentamicin, erythromycin, and cefoxitin. According to sample evaluation results, S. aureus was detected in 94 (94%) of the 100 isolated milk samples based on morphological culture features, Gram staining, and biochemical assays. According to the profile of antibiotic resistance derived from the findings of the S. aureus antibiotic resistance test, 23 isolates (24.47%) were proven to be multidrug-resistant (MDR) because they were resistant to three to four classes of antibiotics. The use of antibiotics typically rises in response to an increase in the prevalence of disease in cattle, which could lead to higher levels of antibiotic residue in milk and possibly higher levels of bacterial resistance to antibiotics. Therefore, dairy farming requires methods for prudently and correctly employing antibiotics.

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“…Of course, in the disposal phase, only recycling options are presented, but more scenarios can be predicted like the landfill or a scenario where only half of the materials will be recycled. However, regarding the regulations of the Waste Electrical and Electronic Equipment Directive (WEEE) [55], it should be collected by dedicated companies, and because of this, this scenario is presented as the most probable. In the article, the LCA analysis should show the main value of LCA and that everything has an impact on the environment; even people who think about our health and that it is the most important issue, we always have an impact on the environment.…”

Section: Lca-the Ecological Cost Of Emission Reductionmentioning

confidence: 99%

“…Staphylococci are abundant bacteria of the human skin microbiome [55] that often do not cause infections. However, in certain circumstances, they may pose a threat to both immunocompetent people and people whose immune system is weakened or impaired [1].…”

Section: Concentrations Of Mannitol-positive Staphylococci and Their ...mentioning

confidence: 99%

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Health Effects and Exposure Assessment to Bioaerosols in Indoor and Outdoor Environments

2021

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Prevalence and antibiogram of coagulase negative Staphylococci in bioaerosols from different indoors of a university in India

Abstract: Background: Staphylococci species are the major constituents of infectious bioaerosols, particularly methicillinresistant Staphylococci (MRS) have serious health impacts. Here, the bacterial burden was quantified, especially

Cited by 8 publications

References 53 publications

Microbiological Air Quality in a Highschool Gym Located in an Urban Area of Southern Poland—Preliminary Research

Microbiological Air Quality in a Highschool Gym Located in an Urban Area of Southern Poland—Preliminary Research

Detection of multidrug-resistant Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia

Health Effects and Exposure Assessment to Bioaerosols in Indoor and Outdoor Environments

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