Pretreatment with probiotic Bifico ameliorates colitis‐associated cancer in mice: Transcriptome and gut flora profiling

Abstract

Individuals with inflammatory bowel disease are at high risk of developing colitis‐associated cancer (CAC). Strategies to block the process from inflammatory bowel disease to CAC should be considered. In the experiment, we aim to explore the chemopreventive efficacy of the probiotic cocktail Bifico and its potential mechanism in azoxymethane and dextran sodium sulphate‐induced CAC in mice. Oral pretreatment of Bifico was adopted to evaluate its protective effect. The colorectums of 35 C57BL/6 mice were collected and examined for the degree of inflammation and tumorigenesis. Comparative 16S rRNA sequencing was carried out to observe Bifico‐target alterations in gene expression and microbiota structure. We found that pretreatment of Bifico alleviated intestinal inflammation and reduced tumor formation. Furthermore, we identified a subset of genes as potential targets of Bifico treatment, including CXCL1,CXCL2,CXCL3, and CXCL5, which are all ligands of C‐X‐C motif receptor 2 (CXCR2). The 16S rRNA sequencing showed that Bifico decreased the abundance of genera Desulfovibrio, Mucispirillum, and Odoribacter, and a bloom of genus Lactobacillus was detected. Notably, we found that an abundance of these Bifico‐target taxa was significantly associated with the expression of CXCR2 ligand genes. Our studies indicate that Bifico, given orally, can ameliorate CAC in mice through intervening with the possible link between Desulfovibrio, Mucispirillum, Odoribacter, Lactobacillus, and CXCR2 signaling.

A Galacto-Oligosaccharides Preparation Derived From Lactulose Protects Against Colorectal Cancer Development in an Animal Model

“…This bacteria have been associated to apoptosis induction in the colon mucosa in in vitro models and to a pro-inflammatory changes in UC patients, such as decreased sulphation of mucin, as a result of the metabolic activity of these bacteria ( Lennon et al, 2014 ; Coutinho et al, 2017 ). Also, this bacterial genus has been associated to increasing levels of damage (also toward CRC) at the mucosal level, caused by reduction of the mucin barrier, the natural protection against luminal microbiota pro-inflammatory challenges ( Song et al, 2018 ). However, another member of this family, Bilophila wadsworthia , is increased in GOS-Lu cohort.…”

The Role of Probiotics in Colorectal Cancer Management

“…Song et al [47] studied the effect of probiotic Bifico on azoxymethane/dextran sodium sulfate-induced colitis-associated carcinogenesis in C57BL/6 mice. e supplementation of probiotic mix (B. longum, L. acidophilus, and E. faecalis; 1.2 × 10 7 CFU/day for 2 weeks pretreatment and continued till the end of the experiment) significantly reduced the tumor formation and intestinal inflammation, and improved the diversity and abundance of microbiota and altered the expression of CXCR2 ligand genes in azoxymethane/dextran sodium sulfate-induced colitis-associated cancer mice model [47].…”

Switching to a Healthy Diet Prevents the Detrimental Effects of Western Diet in a Colitis-Associated Colorectal Cancer Model

“…Lactic acid producing bacteria are used as probiotics and can support the growth of tumor-suppressive short fatty acid-producing bacteria [89]. Additionally, WD reduced the amount of Ruminoclostridium, which were inversely related to colorectal cancer [90,91].…”