Pretreatment of surfactant Tween 80 and fibrolytic enzyme influencing volatile fatty acid and methane production of a total mixed ration by mixed rumen microorganisms at in vitro

Abstract

In vitro rumen dry matter degradability, volatile fatty acid (VFA) and methane production were examined in a total mixed ration pretreated with different levels of either commercial cellulolytic enzyme (CCE), the surfactant poly oxyethylene sorbitan monoolate (Tween 80) or both: (i) no pretreatment (Control); (ii) with 1% CCE alone; (iii) with 2% CCE alone; (iv) with 1% Tween 80 alone; (v) with 1% Tween 80 + 1% CCE;(vi) with 1% Tween 80 + 2% CCE; (vii) with 2% Tween 80 alone; (viii) with 2% Tween 80 + 1% CCE; and (ix) with 2% Tween 80 +2% CCE. In all culture supernatants of rumen microorganisms, activities of xylanase and cellulase were significantly (P < 0.001) increased with pretreatment of CCE alone, Tween 80 alone and both combined. Within the same treatment of no CCE and 1% CCE levels, the total VFA concentration was significantly (P < 0.001) higher with 2% Tween 80 than with no or 1% Tween 80 treatments. The molar percentage of propionate significantly (P < 0.001) increased only with Tween 80. Throughout the in vitro incubation, the average value of methane concentration decrease was greater (P < 0.001) with 1% CCE treatment than with no CCE and 2% CCE treatments, accounting for 0.797 and 0.887 of no CCE and 2% CCE treatments, respectively. Accumulative methane production was significantly lower with 1% CCE treatment than with no CCE and 2% CCE treatments. A ratio of VFA : methane production was significantly (P < 0.001) higher with 2% Tween 80 treatment than with no Tween 80 and 1% Tween 80 treatments, showing the average values of 7.28, 5.33 and 6.69, respectively.

Surfactant‐supplemented mixed bacterial cultures to produce hydrogen from paperboard mill wastewater

“…These results are comparable to those obtained by Kim et al. who found that HAc/HPr decreased from 2 to 1.8 by increasing T80 concentration from 1 to 2%, respectively. …”

“…2). These results are comparable to those obtained by Kim et al [35] who found that HAc/HPr decreased from 2 to 1.8 by increasing T80 concentration from 1 to 2%, respectively.…”

“…This is not the case for usage of T80 that distinctly can bind with the PMW complex organics and therefore breaks it into simpler organics and subsequently increases the HP. Furthermore, addition of T80 improved enzymatic degradation of PMW . Qing et al.…”

“…This is not the case for usage of T80 that distinctly can bind with the PMW complex organics and therefore breaks it into simpler organics [15] and subsequently increases the HP. Furthermore, addition of T80 improved enzymatic degradation of PMW [35]. Qing et al [13] found that the addition of T80 enhanced the lignin degradation and facilitated the enzymes access for cellulose and hemicelluloses that eventually enhanced the HYs.…”

Effect of unconventional oilseeds (safflower, poppy, hemp, camelina) on in vitro ruminal methane production and fermentation

“…Before use, the coconut oil was emulsified in a 1:99 v/v aqueous solution of Tween 80 (Sigma–Aldrich, St. Louis, MO, USA) to allow its homogenous dispersal in the mixture inoculum. Tween 80 has previously been shown to have a minimal and inconsistent effect on biohydrogenation and methane production . Therefore, Tween 80 was not expected to have a significant effect on ruminal fermentation and no Tween 80‐only control was used.…”