Pretreatment Of Nano Chitosan And Nano Calcium (X. gideon) In The Application Of Acetic Acid To Enamel Hardness

Komariah, Ade; Parcelia, Selly; Trenggono, Bambang S.

doi:10.33945/sami/ijabbr.2019.3.6

Cited by 2 publications

(2 citation statements)

References 10 publications

(10 reference statements)

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“…The source of chitosan in this study was X. gideon obtained from Cangkurawok, Damaga, and Balumbang Jaya, Bo gor, East Java. All parts of X. gideon body were detached, followed by drying for five days, and then continied to the processes of demineralization (3N HCl), deproteinization (3N NaOH), discoloration (4% H 2 O 2 ) and deacetylation (50% NaOH) (Komariah et al 2019).…”

Section: Polymeric Materialsmentioning

confidence: 99%

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Komariah,

Trisfilha,

Wahyudi

et al. 2023

Indones. J. Biotechnol.

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During phagocytosis, phagocyte cells discharge reactive oxygen species referred to as respiratory bursts, inducing a rise in pro‐oxidants and subjecting the cell to oxidative stress. Such stress is a biological mechanism related to an imbalance in pro‐oxidant/antioxidant homeostasis, which generates toxic reactive oxygen. Encapsulation is a coating process to improve the stability of bioactive compounds from lemongrass extract. Therefore, this study aims to determine the encapsulation activity of lemongrass leaf extract with chitosan X. gideon (LEChXg) to reduce the oxidative stress of fibroblasts. The research used the human dermal fibroblast (HDF) cell line, comprising negative and positive controls and use of LEChXg 100, 200, 300, 400, and 500 µg/mL. HDF cell migration was evaluated by employing the scratch wound healing method and the wound closure was oberseved at 0, 2, 4, 6, and 24 h intervals. The cell proliferation was observed at 24, 48, and 72 h using CCK‐8 at a 450 nm wavelength. The results showed that the observations at 0, 2, and 4 h did not demonstrate any significant difference on the cell migration (p > 0.05) among the groups. However, the wound closure at 4 and 6 h showed a significant difference (p < 0.05) with LEChXg 300 µg/mL. Despite the lack of any significant variation observed up to 24 h, fibroblast subjected to the stressor did not achieve complete closure. The groups treated with LEChXg were more stable in maintaining fibroblast proliferation up to the end of the observation than those with stressors at 24, 48, and 72 h. Fibroblast induced with a stressor was also more stable in maintaining migration and proliferation in groups receiving LEChXg 300 µg/mL.

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Section: Polymeric Materialsmentioning

confidence: 99%

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Komariah,

Trisfilha,

Wahyudi

et al. 2023

Indones. J. Biotechnol.

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“…Also, biofilm is one of the reasons for antibiotic resistance and failure to treat bacterial infections [14]. Any disruption of this mechanism can result in pathological circumstances, which can lead to a number of disorders affecting the soft and hard tissues of the mouth [15]. Normal flora in the oral cavity is nonpathogenic and has a stable relationship with the host.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2023

J. Med. Chem. Sci.

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Osseointegrated dental implants are becoming more common as an option for replacing the missing or damaged teeth. Peri-implant infections are caused by anaerobic Gram-negative bacteria. This may initiate an inflammatory release of cytokines that will enhance the accumulation of neutrophils in the implant lesion that is essential for the inflammatory and degenerative processes in periodontal tissues. Eighty subjects were enrolled in this study, 40 with peri-implant mucositis and 40 implants that were successfully integrated (42 males and 38 females). The same 40 patients were followed up after three weeks to monitor and observe the progression of inflammation around the implant. Peri-implant sulcular fluid (PISF) samples were collected by perio-paper from all subjects attending the department of Oral and Maxillofacial Surgery at AL-Karama Specialized Dental Center and AL-Ma'amoun Specialized Dental Center, Baghdad, Iraq. (PISF) samples were examined and identified by using the enzyme-linked immunosorbent assay (ELISA) technique for bacterial lipopolysaccharide (LPS). Compared with patients with a successful implant, those with periimplant mucositis exhibited a higher level of lipopolysaccharide (LPS) in PISF (P=0.00000), and then declined significantly following 3 weeks of adequate oral hygiene instructions. The current study concluded that lipopolysaccharide (LPS) reflect, associate, and predict well with the clinical disease activity and progression of peri-implant mucositis.

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Pretreatment Of Nano Chitosan And Nano Calcium (X. gideon) In The Application Of Acetic Acid To Enamel Hardness

Cited by 2 publications

References 10 publications

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

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