Pretreatment of indian cane molasses for increased production of citric acid

Kundu, Subrata; Panda, T.; Majumdar, S. K.; Guha, B.K.; Bandyopadhyay, K. K.

doi:10.1002/bit.260260915

Cited by 38 publications

(12 citation statements)

References 8 publications

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“…The superiority of the TPSA Fig. 1 Effects of different carbon sources on L-lysine production Bioprocess Biosyst Eng treatment over other pretreatment methods in L-lysine fermentation may be owing to the significant removal of heavy metals, pigment, and suspended substances from molasses [8,12,13].…”

Section: Effects Of Pretreatments On L-lysine Productionmentioning

confidence: 99%

“…In the sulfuric acid pretreatment process (SA), the pH of the beet molasses was adjusted to 3.0 [8,12] by adding 1 mol/L sulfuric acid solution, and then allowed to stand for 1 h, followed by centrifugation at 8049g for 10 min. The supernatant obtained was stored at 4°C and used for fermentation.…”

Section: Sulfuric Acid Pretreatmentmentioning

confidence: 99%

“…Previous studies have shown that some chemical substances, such as pigment substances, suspended colloids, excessive metallic ions, and ash, are inevitably generated during molasses refining processes, which could inhibit the growth of microorganisms, influence the pH of the substrate, and inactivate the enzymes associated with the biosynthesis of the product [8,12,13], decreasing the levels of biomass and biochemicals produced. Hence, various pretreatment methods that could selectively remove these hazardous components and fermentation conditions have been examined to optimize microbial growth and biochemical production.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced l-lysine production from pretreated beet molasses by engineered Escherichia coli in fed-batch fermentation

Chen

et al. 2015

Bioprocess Biosyst Eng

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Faster sugar consumption rate and low-cost nitrogen source are required for the chemical biosynthesis using molasses. Five pretreatment methods were applied to beet molasses prior to fermentation through engineered Escherichia coli, respectively, and corn steep liquid was used as an organic nitrogen source to replace expensive yeast extract. Furthermore, the effects of different feeding strategy in fed-batch fermentation on L-lysine production were investigated. The experimental results showed that combined tricalcium phosphate, sulfuric acid, and activated carbon pretreatment method (TPSA) pretreatment could improve the sugar consumption rate most greatly, and the initial total sugar concentration of 35 g/L from TPSA-pretreated beet molasses gave the best results with respect to L-lysine production, dry cell weight concentration, and L-lysine yield in batch fermentation. Moreover, a mixture of low-cost corn steep liquid and yeast extract containing equal amount of nitrogen could be used as the organic nitrogen source for effective L-lysine fermentation, and constant speed feeding strategy of TPSA-pretreated beet molasses promoted L-lysine production by engineered E. coli. The TPSA-pretreated beet molasses had a sugar consumption rate of 1.75 g/(L h), and a L-lysine yield of 27.81% was achieved, compared with the theoretical yield of 62% by glucose. It was clarified that the pretreatment significantly enhanced the conversion of sugars in beet molasses to L-lysine.

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Section: Effects Of Pretreatments On L-lysine Productionmentioning

confidence: 99%

Section: Sulfuric Acid Pretreatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced l-lysine production from pretreated beet molasses by engineered Escherichia coli in fed-batch fermentation

Chen

et al. 2015

Bioprocess Biosyst Eng

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“…During pretreatment process, contaminant microorganisms could also be eliminated from the medium along with suspended inorganic and organic fermentation inhibitory compounds, eventually leading to greater conversion effi ciency. Pretreatment of cane molasses with tricalcium phosphate, hydrochloric acid, and Sephadex fractionation was shown to increased production of citric acid by Aspergillus niger [10]. About 18% increase in ethanol production and 42% reduction in the residual sugars was reported in a batch ethanolic fermentation of pretreated molasses by ceramic microfi ltration membrane [11].…”

Section: Effect Of Pretreatmentmentioning

confidence: 99%

High gravity fermentation of sugarcane molasses to produce ethanol: Effect of nutrients

Pradeep

Obulam

2010

Indian J Microbiol

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Fermentation effi ciency of more than 85% was obtained by high gravity fermentation of 33-34°Bx (spec. gravity ≈1.134) molasses medium with certain nutrients, instead of generally employed medium containing ≈16% (w/v) total sugar (spec. gravity ≈1.090) for ethanol fermentation in distilleries to get maximum 80-85% conversion. The fermenting yeast, Saccharomyces, has varied capabilities, depending on the species and nutrition for fermenting the high solids medium. The fermentation period was reduced to 48 h; and the byproducts obtained were less in concentration, upon supplementation with nutrients (or osmoprotectants) like soy fl our/wheat bran to the medium in the existing batch fermentation technology. This has been found partly due to improved yeast cell viability during fermentation.

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“…Sabe-se que, em certas concentrações, alguns compostos orgânicos e elementos inorgânicos presentes no melaço inibem o crescimento microbiano (KASENO; KOKUGAN, 1997;KUNDU et al, 1984;VADEHRA;GUPTA, 1981 …”

Section: Validação Da Metodologia Para a Determinação Do Teor De Nitrunclassified

Cultivos de<i> Scheffersomyces stipits</i> DSM-3651 visando à produção de etanol lignocelulósico

Santi Junior

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AGRADECIMENTOSÀ Deus, por ter me dado saúde e paz para que eu pudesse ter realizado este meu sonho.Aos meus pais, Celso e Sonia, pelo amor incondicional, apoio e exemplo.À Mariana, minha futura esposa, por todos estes anos de compreensão, amor e cumplicidade.À minha irmã Adriana, pelo apoio, incentivo e amor incondicional.Ao Prof. Walter de Carvalho, pelos ensinamentos, dedicação e orientação ao longo de todos estes anos de iniciação à carreira científica.Aos meus sobrinhos, Julia e Lorenzo, pelos momentos de descontração e alegria.À minha família paulistana, por todo amor, incentivo e suporte.Aos meus "amigos-irmãos" por todo amor e companheirismo desde sempre.À minha segunda família, Molina Souza, por todo amor e apoio.Aos amigos, Victor, Paula e Germano, pela amizade sincera e por todos estes anos de companheirismo. Este estudo buscou definir as melhores condições para a fermentação alcoólica da xilose pela levedura S. stipitis DSM-3651, utilizando suplementação nutricional de baixo custo. Inicialmente, o melaço de cana foi escolhido, devido à sua disponibilidade e inserção no contexto do etanol de segunda geração. Sendo assim, o estudo acerca dos requerimentos nutricionais foi realizado em quatro etapas. A primeira foi avaliar a capacidade da levedura em utilizar os açúcares provenientes de materiais lignocelulósicos e do melaço: xilose, arabinose, glicose, frutose e sacarose. Observou-se que a levedura foi capaz de converter apenas glicose, xilose e frutose em etanol, e inclusive realizar a co-fermentação destes. Desta forma, a segunda etapa foi determinar a composição química do melaço e compará-la com a de um suplemento referência (extratos de levedura e malte, e peptona). Enquanto o conteúdo de minerais foi semelhante o teor de nitrogênio foi bem inferior, de modo que o enriquecimento com fosfato de amônio foi requerido. A próxima etapa confrontou a qualidade nutricional de meios suplementados com melaço e fosfato ou com a suplementação referência, utilizando xilose como fonte de carbono. Embutida nesta análise foram testadas diferentes cargas de inóculo (0,07 e 3,00 g/L) provenientes de cultivos com ou sem limitação de nutrientes. Como resposta obtivemos que, de modo geral, o desempenho fermentativo de S. stipitis DSM-3651 foi reduzido quando apenas melaço e fosfato foram disponibilizados como nutrientes. Desta forma a quarta e última etapa baseou-se na metodologia de planejamento experimental para definir a combinação de nutrientes ideal para cada carga de inóculo. Com base na produção de etanol, definiu-se que, para o prosseguimento do trabalho um meio à base, principalmente, de melaço seria o mais adequado, utilizando uma concentração celular inicial de 3,00 g/L. O estudo dos efeitos das condições de fermentação no crescimento celular e na produção de etanol a partir de xilose, foi realizado com base no planejamento de experimentos e adequada análise estatística, utilizando 4 variáveis: aeração e concentrações de substrato, células e suplemento. Após avaliar e interpretar o impacto que cada variável ...

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Pretreatment of indian cane molasses for increased production of citric acid

Cited by 38 publications

References 8 publications

Enhanced l-lysine production from pretreated beet molasses by engineered Escherichia coli in fed-batch fermentation

Enhanced l-lysine production from pretreated beet molasses by engineered Escherichia coli in fed-batch fermentation

High gravity fermentation of sugarcane molasses to produce ethanol: Effect of nutrients

Cultivos de<i> Scheffersomyces stipits</i> DSM-3651 visando à produção de etanol lignocelulósico

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